Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS05005 and CA265_RS05010 are separated by 286 nucleotides CA265_RS05010 and CA265_RS05015 overlap by 13 nucleotides CA265_RS05015 and CA265_RS05020 are separated by 51 nucleotides
CA265_RS05005: CA265_RS05005 - ion transporter, at 1,188,856 to 1,189,797
_RS05005
CA265_RS05010: CA265_RS05010 - anthranilate phosphoribosyltransferase (EC 2.4.2.18) (from data) , at 1,190,084 to 1,191,073
_RS05010
CA265_RS05015: CA265_RS05015 - N-(5'-phosphoribosyl)anthranilate isomerase, at 1,191,061 to 1,191,696
_RS05015
CA265_RS05020: CA265_RS05020 - tryptophan synthase subunit beta, at 1,191,748 to 1,192,929
_RS05020
Position (kb)
1190
1191
1192 Strain fitness (log2 ratio)
-2
-1
0
1 at 1189.105 kb on + strand, within CA265_RS05005 at 1189.105 kb on + strand, within CA265_RS05005 at 1189.105 kb on + strand, within CA265_RS05005 at 1189.106 kb on - strand, within CA265_RS05005 at 1189.211 kb on - strand, within CA265_RS05005 at 1189.322 kb on - strand, within CA265_RS05005 at 1189.332 kb on + strand, within CA265_RS05005 at 1189.332 kb on + strand, within CA265_RS05005 at 1189.333 kb on - strand, within CA265_RS05005 at 1189.353 kb on - strand, within CA265_RS05005 at 1189.451 kb on + strand, within CA265_RS05005 at 1189.560 kb on - strand, within CA265_RS05005 at 1189.560 kb on - strand, within CA265_RS05005 at 1189.565 kb on - strand, within CA265_RS05005 at 1189.573 kb on + strand, within CA265_RS05005 at 1189.593 kb on - strand, within CA265_RS05005 at 1189.650 kb on - strand, within CA265_RS05005 at 1189.650 kb on - strand, within CA265_RS05005 at 1189.650 kb on - strand, within CA265_RS05005 at 1189.693 kb on - strand, within CA265_RS05005 at 1189.768 kb on - strand at 1189.799 kb on + strand at 1189.849 kb on + strand at 1189.874 kb on + strand at 1190.020 kb on - strand at 1190.032 kb on - strand at 1190.228 kb on - strand, within CA265_RS05010 at 1190.233 kb on - strand, within CA265_RS05010 at 1190.410 kb on + strand, within CA265_RS05010 at 1190.444 kb on - strand, within CA265_RS05010 at 1190.460 kb on + strand, within CA265_RS05010 at 1190.493 kb on + strand, within CA265_RS05010 at 1190.505 kb on + strand, within CA265_RS05010 at 1190.529 kb on + strand, within CA265_RS05010 at 1190.656 kb on - strand, within CA265_RS05010 at 1190.685 kb on + strand, within CA265_RS05010 at 1190.730 kb on + strand, within CA265_RS05010 at 1190.730 kb on + strand, within CA265_RS05010 at 1190.925 kb on + strand, within CA265_RS05010 at 1191.060 kb on + strand at 1191.308 kb on + strand, within CA265_RS05015 at 1191.331 kb on + strand, within CA265_RS05015 at 1191.389 kb on + strand, within CA265_RS05015 at 1191.520 kb on - strand, within CA265_RS05015 at 1191.666 kb on + strand at 1191.694 kb on + strand at 1191.702 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 1,189,105 + CA265_RS05005 0.26 +0.2 1,189,105 + CA265_RS05005 0.26 +0.2 1,189,105 + CA265_RS05005 0.26 +0.1 1,189,106 - CA265_RS05005 0.27 -1.9 1,189,211 - CA265_RS05005 0.38 -1.2 1,189,322 - CA265_RS05005 0.49 +0.3 1,189,332 + CA265_RS05005 0.51 +0.6 1,189,332 + CA265_RS05005 0.51 -0.7 1,189,333 - CA265_RS05005 0.51 +0.2 1,189,353 - CA265_RS05005 0.53 -0.5 1,189,451 + CA265_RS05005 0.63 -0.1 1,189,560 - CA265_RS05005 0.75 -0.6 1,189,560 - CA265_RS05005 0.75 -0.8 1,189,565 - CA265_RS05005 0.75 -0.1 1,189,573 + CA265_RS05005 0.76 -0.2 1,189,593 - CA265_RS05005 0.78 -0.3 1,189,650 - CA265_RS05005 0.84 -1.5 1,189,650 - CA265_RS05005 0.84 -0.2 1,189,650 - CA265_RS05005 0.84 -0.3 1,189,693 - CA265_RS05005 0.89 -0.2 1,189,768 - -0.5 1,189,799 + +0.1 1,189,849 + -0.2 1,189,874 + +0.8 1,190,020 - -0.1 1,190,032 - -0.7 1,190,228 - CA265_RS05010 0.15 -0.4 1,190,233 - CA265_RS05010 0.15 -0.6 1,190,410 + CA265_RS05010 0.33 -0.3 1,190,444 - CA265_RS05010 0.36 +0.2 1,190,460 + CA265_RS05010 0.38 -0.2 1,190,493 + CA265_RS05010 0.41 -1.2 1,190,505 + CA265_RS05010 0.43 -0.9 1,190,529 + CA265_RS05010 0.45 -1.3 1,190,656 - CA265_RS05010 0.58 +0.8 1,190,685 + CA265_RS05010 0.61 +0.0 1,190,730 + CA265_RS05010 0.65 -0.7 1,190,730 + CA265_RS05010 0.65 +1.4 1,190,925 + CA265_RS05010 0.85 +0.8 1,191,060 + -0.4 1,191,308 + CA265_RS05015 0.39 -0.2 1,191,331 + CA265_RS05015 0.42 +0.6 1,191,389 + CA265_RS05015 0.52 -0.4 1,191,520 - CA265_RS05015 0.72 +0.8 1,191,666 + -0.5 1,191,694 + -0.1 1,191,702 + -0.8
Or see this region's nucleotide sequence