Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04980 and CA265_RS04985 overlap by 1 nucleotides CA265_RS04985 and CA265_RS04990 are separated by 40 nucleotides CA265_RS04990 and CA265_RS04995 are separated by 149 nucleotides CA265_RS04995 and CA265_RS05000 are separated by 103 nucleotides
CA265_RS04980: CA265_RS04980 - recombination protein RecR, at 1,184,996 to 1,185,613
_RS04980
CA265_RS04985: CA265_RS04985 - hypothetical protein, at 1,185,613 to 1,186,350
_RS04985
CA265_RS04990: CA265_RS04990 - short chain dehydrogenase, at 1,186,391 to 1,187,209
_RS04990
CA265_RS04995: CA265_RS04995 - hypothetical protein, at 1,187,359 to 1,187,763
_RS04995
CA265_RS05000: CA265_RS05000 - esterase, at 1,187,867 to 1,188,706
_RS05000
Position (kb)
1186
1187
1188 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1185.464 kb on - strand, within CA265_RS04980 at 1185.488 kb on - strand, within CA265_RS04980 at 1185.543 kb on - strand, within CA265_RS04980 at 1185.642 kb on - strand at 1185.721 kb on + strand, within CA265_RS04985 at 1185.825 kb on + strand, within CA265_RS04985 at 1185.831 kb on - strand, within CA265_RS04985 at 1185.840 kb on + strand, within CA265_RS04985 at 1185.845 kb on + strand, within CA265_RS04985 at 1185.885 kb on + strand, within CA265_RS04985 at 1185.970 kb on + strand, within CA265_RS04985 at 1185.993 kb on + strand, within CA265_RS04985 at 1186.026 kb on + strand, within CA265_RS04985 at 1186.026 kb on + strand, within CA265_RS04985 at 1186.027 kb on - strand, within CA265_RS04985 at 1186.085 kb on - strand, within CA265_RS04985 at 1186.138 kb on + strand, within CA265_RS04985 at 1186.139 kb on - strand, within CA265_RS04985 at 1186.143 kb on + strand, within CA265_RS04985 at 1186.185 kb on + strand, within CA265_RS04985 at 1186.186 kb on - strand, within CA265_RS04985 at 1186.274 kb on + strand, within CA265_RS04985 at 1186.277 kb on + strand at 1186.277 kb on + strand at 1186.306 kb on - strand at 1186.348 kb on + strand at 1186.349 kb on - strand at 1186.349 kb on - strand at 1186.349 kb on - strand at 1186.352 kb on - strand at 1186.379 kb on + strand at 1186.379 kb on + strand at 1186.384 kb on + strand at 1186.387 kb on + strand at 1186.387 kb on + strand at 1186.388 kb on - strand at 1186.410 kb on + strand at 1186.658 kb on - strand, within CA265_RS04990 at 1186.658 kb on - strand, within CA265_RS04990 at 1186.696 kb on - strand, within CA265_RS04990 at 1186.701 kb on + strand, within CA265_RS04990 at 1186.793 kb on - strand, within CA265_RS04990 at 1186.806 kb on + strand, within CA265_RS04990 at 1186.926 kb on + strand, within CA265_RS04990 at 1187.068 kb on + strand, within CA265_RS04990 at 1187.087 kb on - strand, within CA265_RS04990 at 1187.091 kb on + strand, within CA265_RS04990 at 1187.091 kb on + strand, within CA265_RS04990 at 1187.199 kb on + strand at 1187.222 kb on + strand at 1187.239 kb on + strand at 1187.252 kb on + strand at 1187.297 kb on - strand at 1187.300 kb on + strand at 1187.351 kb on - strand at 1187.372 kb on + strand at 1187.373 kb on - strand at 1187.415 kb on + strand, within CA265_RS04995 at 1187.415 kb on + strand at 1187.415 kb on + strand, within CA265_RS04995 at 1187.416 kb on - strand, within CA265_RS04995 at 1187.557 kb on + strand, within CA265_RS04995 at 1187.594 kb on - strand, within CA265_RS04995 at 1187.823 kb on - strand at 1187.855 kb on + strand at 1187.867 kb on - strand at 1187.870 kb on + strand at 1187.871 kb on - strand at 1187.886 kb on + strand at 1187.887 kb on - strand at 1188.074 kb on + strand, within CA265_RS05000 at 1188.075 kb on - strand, within CA265_RS05000 at 1188.111 kb on + strand, within CA265_RS05000 at 1188.112 kb on - strand, within CA265_RS05000 at 1188.116 kb on - strand, within CA265_RS05000 at 1188.124 kb on + strand, within CA265_RS05000 at 1188.124 kb on + strand, within CA265_RS05000 at 1188.126 kb on + strand, within CA265_RS05000 at 1188.131 kb on + strand, within CA265_RS05000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 1,185,464 - CA265_RS04980 0.76 -0.9 1,185,488 - CA265_RS04980 0.80 -0.4 1,185,543 - CA265_RS04980 0.89 -1.8 1,185,642 - +0.9 1,185,721 + CA265_RS04985 0.15 -0.1 1,185,825 + CA265_RS04985 0.29 +0.0 1,185,831 - CA265_RS04985 0.30 +0.1 1,185,840 + CA265_RS04985 0.31 +0.4 1,185,845 + CA265_RS04985 0.31 +0.4 1,185,885 + CA265_RS04985 0.37 -0.5 1,185,970 + CA265_RS04985 0.48 +0.2 1,185,993 + CA265_RS04985 0.51 +0.6 1,186,026 + CA265_RS04985 0.56 +0.8 1,186,026 + CA265_RS04985 0.56 -0.2 1,186,027 - CA265_RS04985 0.56 +0.9 1,186,085 - CA265_RS04985 0.64 +0.1 1,186,138 + CA265_RS04985 0.71 +0.2 1,186,139 - CA265_RS04985 0.71 -0.5 1,186,143 + CA265_RS04985 0.72 +0.8 1,186,185 + CA265_RS04985 0.78 -0.1 1,186,186 - CA265_RS04985 0.78 -0.1 1,186,274 + CA265_RS04985 0.90 -0.8 1,186,277 + +1.3 1,186,277 + +1.0 1,186,306 - +0.6 1,186,348 + -0.1 1,186,349 - +0.0 1,186,349 - +0.4 1,186,349 - -0.1 1,186,352 - -0.0 1,186,379 + -0.3 1,186,379 + -0.1 1,186,384 + +0.7 1,186,387 + -0.6 1,186,387 + -0.3 1,186,388 - -0.7 1,186,410 + -1.5 1,186,658 - CA265_RS04990 0.33 -3.0 1,186,658 - CA265_RS04990 0.33 -0.9 1,186,696 - CA265_RS04990 0.37 -2.9 1,186,701 + CA265_RS04990 0.38 -3.3 1,186,793 - CA265_RS04990 0.49 -3.4 1,186,806 + CA265_RS04990 0.51 +1.0 1,186,926 + CA265_RS04990 0.65 -3.0 1,187,068 + CA265_RS04990 0.83 -1.6 1,187,087 - CA265_RS04990 0.85 -2.3 1,187,091 + CA265_RS04990 0.85 -2.3 1,187,091 + CA265_RS04990 0.85 -0.2 1,187,199 + +0.1 1,187,222 + +0.1 1,187,239 + +0.6 1,187,252 + +0.0 1,187,297 - -0.3 1,187,300 + -0.3 1,187,351 - +0.2 1,187,372 + -0.2 1,187,373 - +0.2 1,187,415 + CA265_RS04995 0.14 -0.3 1,187,415 + +2.4 1,187,415 + CA265_RS04995 0.14 +1.7 1,187,416 - CA265_RS04995 0.14 -0.1 1,187,557 + CA265_RS04995 0.49 +1.2 1,187,594 - CA265_RS04995 0.58 -0.2 1,187,823 - -0.5 1,187,855 + -0.3 1,187,867 - -0.0 1,187,870 + +0.5 1,187,871 - +0.1 1,187,886 + -1.1 1,187,887 - -1.0 1,188,074 + CA265_RS05000 0.25 +0.9 1,188,075 - CA265_RS05000 0.25 -0.5 1,188,111 + CA265_RS05000 0.29 +2.4 1,188,112 - CA265_RS05000 0.29 +0.2 1,188,116 - CA265_RS05000 0.30 -0.4 1,188,124 + CA265_RS05000 0.31 -0.1 1,188,124 + CA265_RS05000 0.31 -0.5 1,188,126 + CA265_RS05000 0.31 +0.6 1,188,131 + CA265_RS05000 0.31 +0.6
Or see this region's nucleotide sequence