Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04380 and CA265_RS04385 are separated by 93 nucleotides CA265_RS04385 and CA265_RS04390 are separated by 515 nucleotides
CA265_RS04380: CA265_RS04380 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 1,051,656 to 1,052,981
_RS04380
CA265_RS04385: CA265_RS04385 - hypothetical protein, at 1,053,075 to 1,053,761
_RS04385
CA265_RS04390: CA265_RS04390 - histidine phosphatase family protein, at 1,054,277 to 1,054,774
_RS04390
Position (kb)
1053
1054 Strain fitness (log2 ratio)
-2
-1
0
1 at 1053.033 kb on - strand at 1053.068 kb on - strand at 1053.068 kb on - strand at 1053.068 kb on - strand at 1053.099 kb on - strand at 1053.277 kb on - strand, within CA265_RS04385 at 1053.277 kb on - strand, within CA265_RS04385 at 1053.288 kb on - strand, within CA265_RS04385 at 1053.446 kb on + strand, within CA265_RS04385 at 1053.525 kb on + strand, within CA265_RS04385 at 1053.526 kb on - strand, within CA265_RS04385 at 1053.526 kb on - strand, within CA265_RS04385 at 1053.648 kb on - strand, within CA265_RS04385 at 1053.648 kb on - strand, within CA265_RS04385 at 1053.681 kb on - strand, within CA265_RS04385 at 1053.741 kb on - strand at 1053.744 kb on - strand at 1053.744 kb on - strand at 1053.744 kb on - strand at 1053.747 kb on - strand at 1053.747 kb on - strand at 1053.749 kb on - strand at 1053.774 kb on + strand at 1053.825 kb on - strand at 1053.869 kb on - strand at 1053.871 kb on - strand at 1053.905 kb on + strand at 1053.911 kb on + strand at 1053.911 kb on + strand at 1053.912 kb on - strand at 1053.925 kb on + strand at 1053.965 kb on + strand at 1054.069 kb on - strand at 1054.122 kb on - strand at 1054.132 kb on + strand at 1054.132 kb on + strand at 1054.133 kb on - strand at 1054.137 kb on + strand at 1054.201 kb on + strand at 1054.202 kb on - strand at 1054.269 kb on - strand at 1054.334 kb on - strand, within CA265_RS04390 at 1054.399 kb on - strand, within CA265_RS04390 at 1054.415 kb on + strand, within CA265_RS04390 at 1054.415 kb on + strand, within CA265_RS04390 at 1054.559 kb on - strand, within CA265_RS04390 at 1054.617 kb on - strand, within CA265_RS04390 at 1054.617 kb on - strand, within CA265_RS04390 at 1054.761 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 1,053,033 - +0.8 1,053,068 - -0.5 1,053,068 - +0.2 1,053,068 - +0.2 1,053,099 - -0.2 1,053,277 - CA265_RS04385 0.29 -0.6 1,053,277 - CA265_RS04385 0.29 -0.2 1,053,288 - CA265_RS04385 0.31 -1.5 1,053,446 + CA265_RS04385 0.54 +0.2 1,053,525 + CA265_RS04385 0.66 -0.9 1,053,526 - CA265_RS04385 0.66 -0.8 1,053,526 - CA265_RS04385 0.66 -0.2 1,053,648 - CA265_RS04385 0.83 +0.2 1,053,648 - CA265_RS04385 0.83 -0.4 1,053,681 - CA265_RS04385 0.88 -1.0 1,053,741 - +0.0 1,053,744 - -0.2 1,053,744 - -1.2 1,053,744 - -0.9 1,053,747 - -0.6 1,053,747 - -0.2 1,053,749 - -1.3 1,053,774 + -0.4 1,053,825 - -1.1 1,053,869 - -0.9 1,053,871 - +0.7 1,053,905 + -1.1 1,053,911 + -0.8 1,053,911 + +0.1 1,053,912 - -0.3 1,053,925 + -0.4 1,053,965 + +0.3 1,054,069 - -1.8 1,054,122 - -0.1 1,054,132 + +0.5 1,054,132 + -0.3 1,054,133 - -1.4 1,054,137 + +0.7 1,054,201 + +0.6 1,054,202 - -0.9 1,054,269 - +0.0 1,054,334 - CA265_RS04390 0.11 +0.4 1,054,399 - CA265_RS04390 0.24 -1.1 1,054,415 + CA265_RS04390 0.28 -0.5 1,054,415 + CA265_RS04390 0.28 +0.1 1,054,559 - CA265_RS04390 0.57 -0.2 1,054,617 - CA265_RS04390 0.68 +0.0 1,054,617 - CA265_RS04390 0.68 -0.7 1,054,761 - -2.2
Or see this region's nucleotide sequence