Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03730 and CA265_RS03735 overlap by 4 nucleotides CA265_RS03735 and CA265_RS03740 are separated by 42 nucleotides
CA265_RS03730: CA265_RS03730 - glycosyltransferase, at 886,519 to 887,487
_RS03730
CA265_RS03735: CA265_RS03735 - hypothetical protein, at 887,484 to 888,272
_RS03735
CA265_RS03740: CA265_RS03740 - hypothetical protein, at 888,315 to 889,862
_RS03740
Position (kb) 887
888
889 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 887.456 kb on + strand at 887.576 kb on + strand, within CA265_RS03735 at 887.620 kb on + strand, within CA265_RS03735 at 887.664 kb on - strand, within CA265_RS03735 at 887.735 kb on - strand, within CA265_RS03735 at 887.798 kb on + strand, within CA265_RS03735 at 887.813 kb on + strand, within CA265_RS03735 at 887.813 kb on + strand, within CA265_RS03735 at 887.813 kb on + strand, within CA265_RS03735 at 887.821 kb on - strand, within CA265_RS03735 at 887.909 kb on + strand, within CA265_RS03735 at 887.993 kb on + strand, within CA265_RS03735 at 888.038 kb on + strand, within CA265_RS03735 at 888.039 kb on - strand, within CA265_RS03735 at 888.117 kb on + strand, within CA265_RS03735 at 888.230 kb on + strand at 888.230 kb on + strand at 888.231 kb on - strand at 888.257 kb on - strand at 888.296 kb on - strand at 888.371 kb on + strand at 888.382 kb on + strand at 888.571 kb on - strand, within CA265_RS03740 at 888.605 kb on - strand, within CA265_RS03740 at 888.752 kb on + strand, within CA265_RS03740 at 888.863 kb on - strand, within CA265_RS03740 at 888.866 kb on - strand, within CA265_RS03740 at 888.866 kb on - strand, within CA265_RS03740 at 888.905 kb on - strand, within CA265_RS03740 at 889.024 kb on - strand, within CA265_RS03740 at 889.098 kb on + strand, within CA265_RS03740 at 889.203 kb on + strand, within CA265_RS03740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 887,456 + +0.2 887,576 + CA265_RS03735 0.12 -1.8 887,620 + CA265_RS03735 0.17 -1.5 887,664 - CA265_RS03735 0.23 -1.3 887,735 - CA265_RS03735 0.32 -2.9 887,798 + CA265_RS03735 0.40 -2.3 887,813 + CA265_RS03735 0.42 -2.6 887,813 + CA265_RS03735 0.42 +0.7 887,813 + CA265_RS03735 0.42 -1.3 887,821 - CA265_RS03735 0.43 -2.3 887,909 + CA265_RS03735 0.54 -2.5 887,993 + CA265_RS03735 0.65 -0.4 888,038 + CA265_RS03735 0.70 -2.2 888,039 - CA265_RS03735 0.70 -3.7 888,117 + CA265_RS03735 0.80 -0.2 888,230 + -0.7 888,230 + -0.7 888,231 - -0.9 888,257 - -3.3 888,296 - -0.8 888,371 + -1.9 888,382 + -1.6 888,571 - CA265_RS03740 0.17 -2.9 888,605 - CA265_RS03740 0.19 -1.9 888,752 + CA265_RS03740 0.28 -0.9 888,863 - CA265_RS03740 0.35 -0.5 888,866 - CA265_RS03740 0.36 -1.3 888,866 - CA265_RS03740 0.36 -2.1 888,905 - CA265_RS03740 0.38 -1.5 889,024 - CA265_RS03740 0.46 -3.6 889,098 + CA265_RS03740 0.51 +0.1 889,203 + CA265_RS03740 0.57 -0.5
Or see this region's nucleotide sequence