Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03575 and CA265_RS03580 are separated by 78 nucleotides CA265_RS03580 and CA265_RS03585 are separated by 78 nucleotides CA265_RS03585 and CA265_RS03590 are separated by 323 nucleotides
CA265_RS03575: CA265_RS03575 - hypothetical protein, at 856,361 to 858,310
_RS03575
CA265_RS03580: CA265_RS03580 - acyl-CoA reductase, at 858,389 to 859,405
_RS03580
CA265_RS03585: CA265_RS03585 - ferredoxin, at 859,484 to 859,834
_RS03585
CA265_RS03590: CA265_RS03590 - universal stress protein, at 860,158 to 860,610
_RS03590
Position (kb)
858
859
860 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 857.395 kb on + strand, within CA265_RS03575 at 857.401 kb on + strand, within CA265_RS03575 at 857.402 kb on - strand, within CA265_RS03575 at 857.405 kb on + strand, within CA265_RS03575 at 857.517 kb on + strand, within CA265_RS03575 at 857.572 kb on + strand, within CA265_RS03575 at 857.588 kb on - strand, within CA265_RS03575 at 857.588 kb on - strand, within CA265_RS03575 at 857.645 kb on + strand, within CA265_RS03575 at 857.645 kb on + strand, within CA265_RS03575 at 857.646 kb on - strand, within CA265_RS03575 at 857.680 kb on + strand, within CA265_RS03575 at 857.709 kb on - strand, within CA265_RS03575 at 857.709 kb on - strand, within CA265_RS03575 at 857.751 kb on + strand, within CA265_RS03575 at 857.751 kb on + strand, within CA265_RS03575 at 857.804 kb on + strand, within CA265_RS03575 at 857.880 kb on - strand, within CA265_RS03575 at 857.972 kb on + strand, within CA265_RS03575 at 858.050 kb on - strand, within CA265_RS03575 at 858.050 kb on - strand, within CA265_RS03575 at 858.087 kb on - strand, within CA265_RS03575 at 858.092 kb on - strand, within CA265_RS03575 at 858.101 kb on + strand, within CA265_RS03575 at 858.127 kb on + strand at 858.129 kb on + strand at 858.129 kb on + strand at 858.133 kb on + strand at 858.150 kb on + strand at 858.150 kb on + strand at 858.151 kb on - strand at 858.151 kb on - strand at 858.176 kb on - strand at 858.181 kb on + strand at 858.184 kb on + strand at 858.277 kb on + strand at 858.278 kb on - strand at 858.288 kb on - strand at 858.313 kb on - strand at 858.334 kb on - strand at 858.337 kb on - strand at 858.341 kb on - strand at 858.367 kb on + strand at 858.388 kb on - strand at 858.418 kb on + strand at 858.419 kb on - strand at 858.422 kb on + strand at 858.503 kb on + strand, within CA265_RS03580 at 858.682 kb on - strand, within CA265_RS03580 at 858.692 kb on - strand, within CA265_RS03580 at 858.694 kb on + strand, within CA265_RS03580 at 858.694 kb on + strand, within CA265_RS03580 at 858.703 kb on + strand, within CA265_RS03580 at 858.715 kb on + strand, within CA265_RS03580 at 858.757 kb on + strand, within CA265_RS03580 at 858.757 kb on + strand, within CA265_RS03580 at 858.757 kb on + strand, within CA265_RS03580 at 858.758 kb on - strand, within CA265_RS03580 at 858.870 kb on - strand, within CA265_RS03580 at 858.870 kb on - strand, within CA265_RS03580 at 859.116 kb on - strand, within CA265_RS03580 at 859.121 kb on + strand, within CA265_RS03580 at 859.236 kb on - strand, within CA265_RS03580 at 859.236 kb on - strand, within CA265_RS03580 at 859.328 kb on + strand at 859.841 kb on - strand at 859.964 kb on - strand at 859.964 kb on - strand at 859.990 kb on + strand at 859.990 kb on + strand at 860.023 kb on + strand at 860.024 kb on - strand at 860.083 kb on + strand at 860.136 kb on + strand at 860.137 kb on - strand at 860.159 kb on + strand at 860.209 kb on - strand, within CA265_RS03590 at 860.209 kb on - strand, within CA265_RS03590 at 860.316 kb on + strand, within CA265_RS03590 at 860.344 kb on - strand, within CA265_RS03590 at 860.347 kb on + strand, within CA265_RS03590 at 860.348 kb on - strand, within CA265_RS03590 at 860.348 kb on - strand, within CA265_RS03590 at 860.351 kb on + strand, within CA265_RS03590 at 860.371 kb on - strand, within CA265_RS03590 at 860.403 kb on + strand, within CA265_RS03590
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 857,395 + CA265_RS03575 0.53 +0.4 857,401 + CA265_RS03575 0.53 +0.0 857,402 - CA265_RS03575 0.53 -0.1 857,405 + CA265_RS03575 0.54 +4.3 857,517 + CA265_RS03575 0.59 -0.5 857,572 + CA265_RS03575 0.62 -1.3 857,588 - CA265_RS03575 0.63 -0.7 857,588 - CA265_RS03575 0.63 -0.2 857,645 + CA265_RS03575 0.66 -0.7 857,645 + CA265_RS03575 0.66 -0.5 857,646 - CA265_RS03575 0.66 -0.6 857,680 + CA265_RS03575 0.68 +0.5 857,709 - CA265_RS03575 0.69 -0.3 857,709 - CA265_RS03575 0.69 -0.7 857,751 + CA265_RS03575 0.71 +1.0 857,751 + CA265_RS03575 0.71 +0.8 857,804 + CA265_RS03575 0.74 +0.4 857,880 - CA265_RS03575 0.78 -0.1 857,972 + CA265_RS03575 0.83 -0.7 858,050 - CA265_RS03575 0.87 -0.4 858,050 - CA265_RS03575 0.87 +0.3 858,087 - CA265_RS03575 0.89 -0.2 858,092 - CA265_RS03575 0.89 -0.7 858,101 + CA265_RS03575 0.89 +0.2 858,127 + +0.5 858,129 + +1.9 858,129 + +0.0 858,133 + -1.1 858,150 + +0.4 858,150 + -0.2 858,151 - -0.5 858,151 - +1.7 858,176 - -0.9 858,181 + -1.0 858,184 + -0.3 858,277 + +0.6 858,278 - -2.2 858,288 - -2.2 858,313 - -0.1 858,334 - +0.4 858,337 - -0.3 858,341 - +0.1 858,367 + -0.3 858,388 - -0.5 858,418 + -0.4 858,419 - -0.1 858,422 + -2.2 858,503 + CA265_RS03580 0.11 -1.7 858,682 - CA265_RS03580 0.29 -0.9 858,692 - CA265_RS03580 0.30 -1.7 858,694 + CA265_RS03580 0.30 -0.8 858,694 + CA265_RS03580 0.30 -0.9 858,703 + CA265_RS03580 0.31 -0.5 858,715 + CA265_RS03580 0.32 -0.1 858,757 + CA265_RS03580 0.36 +0.3 858,757 + CA265_RS03580 0.36 -1.8 858,757 + CA265_RS03580 0.36 -1.5 858,758 - CA265_RS03580 0.36 -2.4 858,870 - CA265_RS03580 0.47 -0.7 858,870 - CA265_RS03580 0.47 -0.5 859,116 - CA265_RS03580 0.71 -1.4 859,121 + CA265_RS03580 0.72 -0.5 859,236 - CA265_RS03580 0.83 -0.2 859,236 - CA265_RS03580 0.83 +0.8 859,328 + -0.9 859,841 - -0.3 859,964 - -0.1 859,964 - +0.9 859,990 + +0.1 859,990 + -0.1 860,023 + +0.1 860,024 - +0.1 860,083 + -0.6 860,136 + +0.0 860,137 - -0.2 860,159 + +1.1 860,209 - CA265_RS03590 0.11 +0.5 860,209 - CA265_RS03590 0.11 -0.3 860,316 + CA265_RS03590 0.35 +1.1 860,344 - CA265_RS03590 0.41 -0.1 860,347 + CA265_RS03590 0.42 +0.0 860,348 - CA265_RS03590 0.42 -0.4 860,348 - CA265_RS03590 0.42 -0.1 860,351 + CA265_RS03590 0.43 +0.1 860,371 - CA265_RS03590 0.47 +0.7 860,403 + CA265_RS03590 0.54 -0.4
Or see this region's nucleotide sequence