Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03505 and CA265_RS03510 are separated by 40 nucleotides CA265_RS03510 and CA265_RS03515 are separated by 104 nucleotides CA265_RS03515 and CA265_RS03520 are separated by 71 nucleotides CA265_RS03520 and CA265_RS03525 are separated by 259 nucleotides
CA265_RS03505: CA265_RS03505 - oxidoreductase, at 846,040 to 846,804
_RS03505
CA265_RS03510: CA265_RS03510 - ferredoxin, at 846,845 to 847,177
_RS03510
CA265_RS03515: CA265_RS03515 - Fe-S assembly protein IscX, at 847,282 to 847,524
_RS03515
CA265_RS03520: CA265_RS03520 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 847,596 to 848,123
_RS03520
CA265_RS03525: CA265_RS03525 - hypothetical protein, at 848,383 to 849,213
_RS03525
Position (kb)
847
848 Strain fitness (log2 ratio)
-2
-1
0
1 at 846.807 kb on + strand at 846.807 kb on + strand at 846.807 kb on + strand at 847.175 kb on + strand at 847.176 kb on - strand at 847.176 kb on - strand at 847.221 kb on + strand at 847.221 kb on + strand at 847.223 kb on + strand at 847.223 kb on + strand at 847.333 kb on - strand, within CA265_RS03515 at 847.347 kb on - strand, within CA265_RS03515 at 847.347 kb on - strand, within CA265_RS03515 at 847.348 kb on + strand, within CA265_RS03515 at 847.392 kb on + strand, within CA265_RS03515 at 847.502 kb on - strand at 847.642 kb on + strand at 847.643 kb on - strand at 847.683 kb on - strand, within CA265_RS03520 at 847.713 kb on - strand, within CA265_RS03520 at 847.769 kb on - strand, within CA265_RS03520 at 847.785 kb on - strand, within CA265_RS03520 at 847.785 kb on - strand, within CA265_RS03520 at 847.847 kb on + strand, within CA265_RS03520 at 847.847 kb on + strand, within CA265_RS03520 at 847.909 kb on - strand, within CA265_RS03520 at 847.923 kb on - strand, within CA265_RS03520 at 847.940 kb on - strand, within CA265_RS03520 at 847.940 kb on - strand, within CA265_RS03520 at 847.982 kb on - strand, within CA265_RS03520 at 848.003 kb on + strand, within CA265_RS03520 at 848.003 kb on + strand, within CA265_RS03520 at 848.004 kb on - strand, within CA265_RS03520 at 848.004 kb on - strand, within CA265_RS03520 at 848.004 kb on - strand, within CA265_RS03520 at 848.020 kb on - strand, within CA265_RS03520 at 848.133 kb on + strand at 848.133 kb on + strand at 848.148 kb on + strand at 848.149 kb on - strand at 848.149 kb on - strand at 848.159 kb on + strand at 848.160 kb on - strand at 848.160 kb on - strand at 848.228 kb on - strand at 848.246 kb on + strand at 848.261 kb on + strand at 848.269 kb on + strand at 848.305 kb on + strand at 848.306 kb on - strand at 848.319 kb on - strand at 848.392 kb on + strand at 848.392 kb on + strand at 848.399 kb on + strand at 848.440 kb on + strand at 848.441 kb on - strand at 848.501 kb on - strand, within CA265_RS03525
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 846,807 + +0.3 846,807 + +0.7 846,807 + +0.6 847,175 + +1.6 847,176 - -1.0 847,176 - -0.9 847,221 + -0.1 847,221 + +0.1 847,223 + -0.2 847,223 + +0.4 847,333 - CA265_RS03515 0.21 +0.6 847,347 - CA265_RS03515 0.27 -0.9 847,347 - CA265_RS03515 0.27 -1.5 847,348 + CA265_RS03515 0.27 -1.9 847,392 + CA265_RS03515 0.45 +0.0 847,502 - -2.7 847,642 + +0.2 847,643 - +0.1 847,683 - CA265_RS03520 0.16 -1.3 847,713 - CA265_RS03520 0.22 -0.2 847,769 - CA265_RS03520 0.33 +0.7 847,785 - CA265_RS03520 0.36 -0.5 847,785 - CA265_RS03520 0.36 -1.5 847,847 + CA265_RS03520 0.48 -0.0 847,847 + CA265_RS03520 0.48 +0.7 847,909 - CA265_RS03520 0.59 -0.4 847,923 - CA265_RS03520 0.62 +0.5 847,940 - CA265_RS03520 0.65 +0.3 847,940 - CA265_RS03520 0.65 +0.1 847,982 - CA265_RS03520 0.73 +0.2 848,003 + CA265_RS03520 0.77 -0.6 848,003 + CA265_RS03520 0.77 -1.5 848,004 - CA265_RS03520 0.77 -0.2 848,004 - CA265_RS03520 0.77 +0.0 848,004 - CA265_RS03520 0.77 -0.3 848,020 - CA265_RS03520 0.80 -0.2 848,133 + -0.5 848,133 + -0.3 848,148 + +0.1 848,149 - -0.1 848,149 - -0.0 848,159 + +0.5 848,160 - +0.7 848,160 - +0.0 848,228 - -0.2 848,246 + +0.2 848,261 + -0.2 848,269 + -2.0 848,305 + -0.3 848,306 - -0.3 848,319 - +0.3 848,392 + +0.2 848,392 + -0.4 848,399 + +0.1 848,440 + +0.6 848,441 - -0.9 848,501 - CA265_RS03525 0.14 -0.3
Or see this region's nucleotide sequence