Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03160 and CA265_RS03165 are separated by 115 nucleotides CA265_RS03165 and CA265_RS03170 are separated by 50 nucleotides CA265_RS03170 and CA265_RS03175 are separated by 113 nucleotides
CA265_RS03160: CA265_RS03160 - hydrolase, at 780,789 to 781,433
_RS03160
CA265_RS03165: CA265_RS03165 - hypothetical protein, at 781,549 to 781,923
_RS03165
CA265_RS03170: CA265_RS03170 - short-chain dehydrogenase, at 781,974 to 782,675
_RS03170
CA265_RS03175: CA265_RS03175 - phenylalanine 4-monooxygenase, at 782,789 to 784,570
_RS03175
Position (kb)
781
782
783 Strain fitness (log2 ratio)
-2
-1
0
1 at 781.018 kb on + strand, within CA265_RS03160 at 781.072 kb on + strand, within CA265_RS03160 at 781.072 kb on + strand, within CA265_RS03160 at 781.140 kb on + strand, within CA265_RS03160 at 781.141 kb on - strand, within CA265_RS03160 at 781.145 kb on + strand, within CA265_RS03160 at 781.215 kb on - strand, within CA265_RS03160 at 781.417 kb on - strand at 781.526 kb on - strand at 781.646 kb on - strand, within CA265_RS03165 at 781.677 kb on + strand, within CA265_RS03165 at 781.678 kb on - strand, within CA265_RS03165 at 781.679 kb on + strand, within CA265_RS03165 at 781.680 kb on - strand, within CA265_RS03165 at 781.680 kb on - strand, within CA265_RS03165 at 781.691 kb on - strand, within CA265_RS03165 at 781.693 kb on - strand, within CA265_RS03165 at 781.790 kb on + strand, within CA265_RS03165 at 781.813 kb on - strand, within CA265_RS03165 at 781.820 kb on + strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.852 kb on - strand, within CA265_RS03165 at 781.862 kb on + strand, within CA265_RS03165 at 781.862 kb on + strand, within CA265_RS03165 at 781.863 kb on - strand, within CA265_RS03165 at 781.967 kb on - strand at 781.972 kb on + strand at 782.051 kb on - strand, within CA265_RS03170 at 782.195 kb on + strand, within CA265_RS03170 at 782.195 kb on + strand, within CA265_RS03170 at 782.196 kb on - strand, within CA265_RS03170 at 782.199 kb on + strand, within CA265_RS03170 at 782.267 kb on + strand, within CA265_RS03170 at 782.305 kb on - strand, within CA265_RS03170 at 782.402 kb on + strand, within CA265_RS03170 at 782.403 kb on - strand, within CA265_RS03170 at 782.424 kb on - strand, within CA265_RS03170 at 782.490 kb on + strand, within CA265_RS03170 at 782.511 kb on + strand, within CA265_RS03170 at 782.532 kb on - strand, within CA265_RS03170 at 782.558 kb on + strand, within CA265_RS03170 at 782.665 kb on - strand at 782.743 kb on - strand at 782.922 kb on + strand at 782.932 kb on + strand at 782.993 kb on - strand, within CA265_RS03175 at 783.065 kb on + strand, within CA265_RS03175 at 783.066 kb on - strand, within CA265_RS03175 at 783.128 kb on + strand, within CA265_RS03175 at 783.133 kb on + strand, within CA265_RS03175 at 783.259 kb on + strand, within CA265_RS03175 at 783.304 kb on + strand, within CA265_RS03175 at 783.304 kb on + strand, within CA265_RS03175 at 783.305 kb on - strand, within CA265_RS03175 at 783.305 kb on - strand, within CA265_RS03175 at 783.305 kb on - strand, within CA265_RS03175 at 783.307 kb on + strand, within CA265_RS03175 at 783.320 kb on - strand, within CA265_RS03175 at 783.327 kb on - strand, within CA265_RS03175 at 783.347 kb on + strand, within CA265_RS03175 at 783.423 kb on - strand, within CA265_RS03175 at 783.434 kb on + strand, within CA265_RS03175 at 783.491 kb on + strand, within CA265_RS03175 at 783.539 kb on - strand, within CA265_RS03175 at 783.559 kb on + strand, within CA265_RS03175 at 783.650 kb on - strand, within CA265_RS03175
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 781,018 + CA265_RS03160 0.36 -0.8 781,072 + CA265_RS03160 0.44 +0.8 781,072 + CA265_RS03160 0.44 -0.8 781,140 + CA265_RS03160 0.54 -0.2 781,141 - CA265_RS03160 0.55 -0.1 781,145 + CA265_RS03160 0.55 -0.7 781,215 - CA265_RS03160 0.66 +0.2 781,417 - -0.2 781,526 - +1.3 781,646 - CA265_RS03165 0.26 -0.4 781,677 + CA265_RS03165 0.34 -0.3 781,678 - CA265_RS03165 0.34 -1.4 781,679 + CA265_RS03165 0.35 -0.2 781,680 - CA265_RS03165 0.35 -0.6 781,680 - CA265_RS03165 0.35 +0.3 781,691 - CA265_RS03165 0.38 +0.2 781,693 - CA265_RS03165 0.38 +1.1 781,790 + CA265_RS03165 0.64 +0.4 781,813 - CA265_RS03165 0.70 -0.6 781,820 + CA265_RS03165 0.72 -0.7 781,821 - CA265_RS03165 0.73 -0.6 781,821 - CA265_RS03165 0.73 -1.1 781,821 - CA265_RS03165 0.73 -0.8 781,821 - CA265_RS03165 0.73 +0.5 781,821 - CA265_RS03165 0.73 -0.2 781,821 - CA265_RS03165 0.73 -1.2 781,852 - CA265_RS03165 0.81 -0.6 781,862 + CA265_RS03165 0.83 +0.1 781,862 + CA265_RS03165 0.83 +1.6 781,863 - CA265_RS03165 0.84 +0.2 781,967 - +0.0 781,972 + +0.4 782,051 - CA265_RS03170 0.11 -0.4 782,195 + CA265_RS03170 0.31 +0.2 782,195 + CA265_RS03170 0.31 -0.6 782,196 - CA265_RS03170 0.32 -0.5 782,199 + CA265_RS03170 0.32 -0.8 782,267 + CA265_RS03170 0.42 -1.0 782,305 - CA265_RS03170 0.47 +0.1 782,402 + CA265_RS03170 0.61 -1.2 782,403 - CA265_RS03170 0.61 +0.4 782,424 - CA265_RS03170 0.64 -1.0 782,490 + CA265_RS03170 0.74 -0.6 782,511 + CA265_RS03170 0.76 -1.7 782,532 - CA265_RS03170 0.79 -1.1 782,558 + CA265_RS03170 0.83 -1.4 782,665 - -0.4 782,743 - -0.6 782,922 + -0.0 782,932 + +0.4 782,993 - CA265_RS03175 0.11 +0.7 783,065 + CA265_RS03175 0.15 -2.0 783,066 - CA265_RS03175 0.16 +0.4 783,128 + CA265_RS03175 0.19 +0.4 783,133 + CA265_RS03175 0.19 -0.2 783,259 + CA265_RS03175 0.26 -0.1 783,304 + CA265_RS03175 0.29 -0.1 783,304 + CA265_RS03175 0.29 -0.0 783,305 - CA265_RS03175 0.29 -0.3 783,305 - CA265_RS03175 0.29 +0.4 783,305 - CA265_RS03175 0.29 +1.2 783,307 + CA265_RS03175 0.29 -1.1 783,320 - CA265_RS03175 0.30 -0.0 783,327 - CA265_RS03175 0.30 +0.2 783,347 + CA265_RS03175 0.31 -1.1 783,423 - CA265_RS03175 0.36 -0.4 783,434 + CA265_RS03175 0.36 +0.1 783,491 + CA265_RS03175 0.39 -0.8 783,539 - CA265_RS03175 0.42 -0.2 783,559 + CA265_RS03175 0.43 +0.0 783,650 - CA265_RS03175 0.48 +0.0
Or see this region's nucleotide sequence