Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01175 and CA265_RS01180 are separated by 6 nucleotides CA265_RS01180 and CA265_RS01185 are separated by 135 nucleotides CA265_RS01185 and CA265_RS01190 are separated by 24 nucleotides CA265_RS01190 and CA265_RS01195 are separated by 18 nucleotides CA265_RS01195 and CA265_RS01200 are separated by 34 nucleotides
CA265_RS01175: CA265_RS01175 - hypothetical protein, at 296,686 to 297,327
_RS01175
CA265_RS01180: CA265_RS01180 - riboflavin biosynthesis protein, at 297,334 to 297,930
_RS01180
CA265_RS01185: CA265_RS01185 - hypothetical protein, at 298,066 to 298,494
_RS01185
CA265_RS01190: CA265_RS01190 - hypothetical protein, at 298,519 to 298,962
_RS01190
CA265_RS01195: CA265_RS01195 - hypothetical protein, at 298,981 to 299,394
_RS01195
CA265_RS01200: CA265_RS01200 - hypothetical protein, at 299,429 to 299,950
_RS01200
Position (kb) 298
299 Strain fitness (log2 ratio) -1
0
1
2 at 297.109 kb on - strand, within CA265_RS01175 at 297.259 kb on - strand, within CA265_RS01175 at 297.259 kb on - strand, within CA265_RS01175 at 297.259 kb on - strand, within CA265_RS01175 at 297.266 kb on + strand at 297.267 kb on - strand at 297.267 kb on - strand at 297.271 kb on + strand at 297.271 kb on + strand at 297.272 kb on - strand at 297.291 kb on + strand at 297.292 kb on - strand at 297.292 kb on - strand at 297.292 kb on - strand at 297.306 kb on + strand at 297.307 kb on - strand at 297.328 kb on + strand at 297.335 kb on + strand at 297.427 kb on - strand, within CA265_RS01180 at 297.451 kb on - strand, within CA265_RS01180 at 297.492 kb on + strand, within CA265_RS01180 at 297.492 kb on + strand, within CA265_RS01180 at 297.493 kb on - strand, within CA265_RS01180 at 297.496 kb on + strand, within CA265_RS01180 at 297.497 kb on - strand, within CA265_RS01180 at 297.517 kb on + strand, within CA265_RS01180 at 297.561 kb on + strand, within CA265_RS01180 at 297.582 kb on + strand, within CA265_RS01180 at 297.619 kb on + strand, within CA265_RS01180 at 297.619 kb on + strand, within CA265_RS01180 at 297.624 kb on - strand, within CA265_RS01180 at 297.650 kb on - strand, within CA265_RS01180 at 297.702 kb on + strand, within CA265_RS01180 at 297.741 kb on + strand, within CA265_RS01180 at 297.742 kb on - strand, within CA265_RS01180 at 297.751 kb on - strand, within CA265_RS01180 at 297.752 kb on + strand, within CA265_RS01180 at 297.752 kb on + strand, within CA265_RS01180 at 297.753 kb on - strand, within CA265_RS01180 at 297.755 kb on + strand, within CA265_RS01180 at 297.756 kb on - strand, within CA265_RS01180 at 297.815 kb on + strand, within CA265_RS01180 at 297.815 kb on + strand, within CA265_RS01180 at 297.820 kb on - strand, within CA265_RS01180 at 297.895 kb on + strand at 297.906 kb on - strand at 297.906 kb on - strand at 297.907 kb on + strand at 297.907 kb on + strand at 297.908 kb on - strand at 297.949 kb on - strand at 298.057 kb on - strand at 298.081 kb on + strand at 298.081 kb on + strand at 298.085 kb on + strand at 298.085 kb on + strand at 298.086 kb on - strand at 298.230 kb on + strand, within CA265_RS01185 at 298.231 kb on - strand, within CA265_RS01185 at 298.271 kb on + strand, within CA265_RS01185 at 298.272 kb on - strand, within CA265_RS01185 at 298.272 kb on - strand, within CA265_RS01185 at 298.272 kb on - strand, within CA265_RS01185 at 298.407 kb on + strand, within CA265_RS01185 at 298.482 kb on + strand at 298.484 kb on + strand at 298.531 kb on - strand at 298.532 kb on + strand at 298.563 kb on + strand at 298.575 kb on + strand, within CA265_RS01190 at 298.583 kb on + strand, within CA265_RS01190 at 298.584 kb on - strand, within CA265_RS01190 at 298.632 kb on - strand, within CA265_RS01190 at 298.634 kb on + strand, within CA265_RS01190 at 298.664 kb on - strand, within CA265_RS01190 at 298.678 kb on - strand, within CA265_RS01190 at 298.691 kb on + strand, within CA265_RS01190 at 298.692 kb on - strand, within CA265_RS01190 at 298.721 kb on + strand, within CA265_RS01190 at 298.743 kb on - strand, within CA265_RS01190 at 298.767 kb on + strand, within CA265_RS01190 at 298.768 kb on - strand, within CA265_RS01190 at 298.768 kb on - strand, within CA265_RS01190 at 298.769 kb on + strand, within CA265_RS01190 at 298.769 kb on + strand, within CA265_RS01190 at 298.837 kb on - strand, within CA265_RS01190 at 298.867 kb on - strand, within CA265_RS01190 at 298.949 kb on - strand at 298.982 kb on + strand at 299.033 kb on - strand, within CA265_RS01195 at 299.045 kb on - strand, within CA265_RS01195 at 299.078 kb on + strand, within CA265_RS01195 at 299.078 kb on + strand, within CA265_RS01195 at 299.082 kb on + strand, within CA265_RS01195 at 299.107 kb on - strand, within CA265_RS01195 at 299.151 kb on + strand, within CA265_RS01195 at 299.152 kb on - strand, within CA265_RS01195 at 299.186 kb on + strand, within CA265_RS01195 at 299.186 kb on + strand, within CA265_RS01195 at 299.250 kb on + strand, within CA265_RS01195 at 299.281 kb on + strand, within CA265_RS01195 at 299.282 kb on - strand, within CA265_RS01195 at 299.282 kb on - strand, within CA265_RS01195 at 299.292 kb on + strand, within CA265_RS01195 at 299.368 kb on - strand at 299.378 kb on - strand at 299.406 kb on + strand at 299.407 kb on - strand at 299.427 kb on - strand at 299.431 kb on - strand at 299.441 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 297,109 - CA265_RS01175 0.66 -1.3 297,259 - CA265_RS01175 0.89 +0.9 297,259 - CA265_RS01175 0.89 -0.7 297,259 - CA265_RS01175 0.89 -0.5 297,266 + -0.4 297,267 - +0.1 297,267 - +0.5 297,271 + -1.1 297,271 + -0.8 297,272 - +0.0 297,291 + +0.5 297,292 - -1.1 297,292 - +1.9 297,292 - +0.3 297,306 + -1.7 297,307 - -0.1 297,328 + -0.1 297,335 + +0.5 297,427 - CA265_RS01180 0.16 -0.5 297,451 - CA265_RS01180 0.20 +0.1 297,492 + CA265_RS01180 0.26 -0.1 297,492 + CA265_RS01180 0.26 -0.1 297,493 - CA265_RS01180 0.27 -1.0 297,496 + CA265_RS01180 0.27 +0.7 297,497 - CA265_RS01180 0.27 +0.1 297,517 + CA265_RS01180 0.31 +0.1 297,561 + CA265_RS01180 0.38 +0.1 297,582 + CA265_RS01180 0.42 -0.1 297,619 + CA265_RS01180 0.48 +0.3 297,619 + CA265_RS01180 0.48 +0.1 297,624 - CA265_RS01180 0.49 +0.4 297,650 - CA265_RS01180 0.53 -0.8 297,702 + CA265_RS01180 0.62 +0.0 297,741 + CA265_RS01180 0.68 -0.0 297,742 - CA265_RS01180 0.68 +0.0 297,751 - CA265_RS01180 0.70 -0.1 297,752 + CA265_RS01180 0.70 +0.6 297,752 + CA265_RS01180 0.70 +0.0 297,753 - CA265_RS01180 0.70 -0.2 297,755 + CA265_RS01180 0.71 -0.7 297,756 - CA265_RS01180 0.71 -0.1 297,815 + CA265_RS01180 0.81 -0.1 297,815 + CA265_RS01180 0.81 -1.1 297,820 - CA265_RS01180 0.81 +0.4 297,895 + -0.9 297,906 - -0.2 297,906 - +0.5 297,907 + -0.3 297,907 + +0.2 297,908 - +0.5 297,949 - +1.0 298,057 - -1.3 298,081 + -0.2 298,081 + -0.1 298,085 + -0.1 298,085 + -0.3 298,086 - -0.7 298,230 + CA265_RS01185 0.38 +0.2 298,231 - CA265_RS01185 0.38 +0.8 298,271 + CA265_RS01185 0.48 +0.2 298,272 - CA265_RS01185 0.48 -1.1 298,272 - CA265_RS01185 0.48 +0.9 298,272 - CA265_RS01185 0.48 +0.3 298,407 + CA265_RS01185 0.79 +1.2 298,482 + +0.2 298,484 + -0.4 298,531 - -0.1 298,532 + +0.4 298,563 + +0.1 298,575 + CA265_RS01190 0.13 -0.2 298,583 + CA265_RS01190 0.14 +0.1 298,584 - CA265_RS01190 0.15 +0.4 298,632 - CA265_RS01190 0.25 -0.7 298,634 + CA265_RS01190 0.26 +0.1 298,664 - CA265_RS01190 0.33 +0.1 298,678 - CA265_RS01190 0.36 +0.0 298,691 + CA265_RS01190 0.39 -0.5 298,692 - CA265_RS01190 0.39 +0.0 298,721 + CA265_RS01190 0.45 -0.5 298,743 - CA265_RS01190 0.50 -0.0 298,767 + CA265_RS01190 0.56 -0.3 298,768 - CA265_RS01190 0.56 -0.1 298,768 - CA265_RS01190 0.56 -0.1 298,769 + CA265_RS01190 0.56 -0.3 298,769 + CA265_RS01190 0.56 +0.0 298,837 - CA265_RS01190 0.72 -0.5 298,867 - CA265_RS01190 0.78 +0.4 298,949 - -1.3 298,982 + +0.3 299,033 - CA265_RS01195 0.13 +1.6 299,045 - CA265_RS01195 0.15 +0.2 299,078 + CA265_RS01195 0.23 +0.7 299,078 + CA265_RS01195 0.23 +0.3 299,082 + CA265_RS01195 0.24 +0.0 299,107 - CA265_RS01195 0.30 +0.1 299,151 + CA265_RS01195 0.41 +0.0 299,152 - CA265_RS01195 0.41 +0.2 299,186 + CA265_RS01195 0.50 -0.5 299,186 + CA265_RS01195 0.50 -1.3 299,250 + CA265_RS01195 0.65 -0.3 299,281 + CA265_RS01195 0.72 -0.4 299,282 - CA265_RS01195 0.73 -0.5 299,282 - CA265_RS01195 0.73 -0.4 299,292 + CA265_RS01195 0.75 +0.1 299,368 - +0.2 299,378 - -0.2 299,406 + -0.8 299,407 - +0.4 299,427 - +0.6 299,431 - -0.5 299,441 - +0.3
Or see this region's nucleotide sequence