Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_66 and Ac3H11_67 overlap by 4 nucleotides Ac3H11_67 and Ac3H11_68 are separated by 115 nucleotides Ac3H11_68 and Ac3H11_69 are separated by 16 nucleotides
Ac3H11_66 - Benzoate transport, ATPase component, at 68,938 to 69,708
_66
Ac3H11_67 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 69,705 to 70,448
_67
Ac3H11_68 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 70,564 to 71,550
_68
Ac3H11_69 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 71,567 to 72,640
_69
Position (kb) 70
71
72 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 69.774 kb on + strand at 69.972 kb on + strand, within Ac3H11_67 at 69.973 kb on - strand, within Ac3H11_67 at 70.507 kb on - strand at 70.826 kb on - strand, within Ac3H11_68 at 71.443 kb on + strand, within Ac3H11_68 at 71.444 kb on - strand, within Ac3H11_68 at 71.488 kb on - strand at 71.750 kb on + strand, within Ac3H11_69 at 71.751 kb on - strand, within Ac3H11_69 at 72.116 kb on + strand, within Ac3H11_69 at 72.116 kb on + strand, within Ac3H11_69 at 72.117 kb on - strand, within Ac3H11_69 at 72.119 kb on + strand, within Ac3H11_69 at 72.119 kb on + strand, within Ac3H11_69 at 72.119 kb on + strand, within Ac3H11_69 at 72.254 kb on + strand, within Ac3H11_69 at 72.255 kb on - strand, within Ac3H11_69 at 72.329 kb on + strand, within Ac3H11_69 at 72.330 kb on - strand, within Ac3H11_69 at 72.414 kb on - strand, within Ac3H11_69 at 72.414 kb on - strand, within Ac3H11_69 at 72.459 kb on - strand, within Ac3H11_69
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 69,774 + +0.1 69,972 + Ac3H11_67 0.36 +0.6 69,973 - Ac3H11_67 0.36 -0.4 70,507 - +0.8 70,826 - Ac3H11_68 0.27 +0.3 71,443 + Ac3H11_68 0.89 +1.3 71,444 - Ac3H11_68 0.89 +1.1 71,488 - +1.0 71,750 + Ac3H11_69 0.17 -1.4 71,751 - Ac3H11_69 0.17 -0.5 72,116 + Ac3H11_69 0.51 -0.3 72,116 + Ac3H11_69 0.51 -1.3 72,117 - Ac3H11_69 0.51 -0.7 72,119 + Ac3H11_69 0.51 -2.3 72,119 + Ac3H11_69 0.51 -0.8 72,119 + Ac3H11_69 0.51 -0.2 72,254 + Ac3H11_69 0.64 +0.4 72,255 - Ac3H11_69 0.64 -0.9 72,329 + Ac3H11_69 0.71 -0.1 72,330 - Ac3H11_69 0.71 -0.3 72,414 - Ac3H11_69 0.79 -0.7 72,414 - Ac3H11_69 0.79 -2.9 72,459 - Ac3H11_69 0.83 +0.0
Or see this region's nucleotide sequence