Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_663 and Ac3H11_664 are separated by 136 nucleotides Ac3H11_664 and Ac3H11_665 are separated by 202 nucleotides
Ac3H11_663 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 19,563 to 21,242
_663
Ac3H11_664 - 5'-methylthioadenosine nucleosidase (EC 3.2.2.16) @ S-adenosylhomocysteine nucleosidase (EC 3.2.2.9), at 21,379 to 22,134
_664
Ac3H11_665 - Magnesium and cobalt transport protein CorA, at 22,337 to 23,323
_665
Position (kb) 21
22
23 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 20.643 kb on + strand, within Ac3H11_663 at 20.643 kb on + strand, within Ac3H11_663 at 20.643 kb on + strand, within Ac3H11_663 at 20.700 kb on + strand, within Ac3H11_663 at 20.701 kb on - strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.736 kb on + strand, within Ac3H11_663 at 20.737 kb on - strand, within Ac3H11_663 at 20.737 kb on - strand, within Ac3H11_663 at 20.737 kb on - strand, within Ac3H11_663 at 20.737 kb on - strand, within Ac3H11_663 at 20.737 kb on - strand, within Ac3H11_663 at 20.737 kb on - strand, within Ac3H11_663 at 20.737 kb on - strand, within Ac3H11_663 at 20.737 kb on - strand, within Ac3H11_663 at 20.737 kb on - strand, within Ac3H11_663 at 20.877 kb on + strand, within Ac3H11_663 at 20.878 kb on - strand, within Ac3H11_663 at 20.976 kb on + strand, within Ac3H11_663 at 20.976 kb on + strand, within Ac3H11_663 at 21.240 kb on + strand at 21.241 kb on - strand at 21.378 kb on + strand at 21.379 kb on - strand at 21.379 kb on - strand at 21.385 kb on - strand at 21.385 kb on - strand at 21.385 kb on - strand at 21.385 kb on - strand at 21.661 kb on + strand, within Ac3H11_664 at 21.661 kb on + strand, within Ac3H11_664 at 21.661 kb on + strand, within Ac3H11_664 at 21.661 kb on + strand, within Ac3H11_664 at 21.661 kb on + strand, within Ac3H11_664 at 21.662 kb on - strand, within Ac3H11_664 at 21.662 kb on - strand, within Ac3H11_664 at 21.662 kb on - strand, within Ac3H11_664 at 21.662 kb on - strand, within Ac3H11_664 at 21.662 kb on - strand, within Ac3H11_664 at 21.662 kb on - strand, within Ac3H11_664 at 21.718 kb on + strand, within Ac3H11_664 at 21.719 kb on - strand, within Ac3H11_664 at 21.995 kb on - strand, within Ac3H11_664 at 22.211 kb on + strand at 22.211 kb on + strand at 22.212 kb on - strand at 22.212 kb on - strand at 22.212 kb on - strand at 22.224 kb on - strand at 22.224 kb on - strand at 22.338 kb on + strand at 22.338 kb on + strand at 22.338 kb on + strand at 22.339 kb on - strand at 22.339 kb on - strand at 22.339 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 20,643 + Ac3H11_663 0.64 +0.6 20,643 + Ac3H11_663 0.64 +0.7 20,643 + Ac3H11_663 0.64 -0.6 20,700 + Ac3H11_663 0.68 +2.2 20,701 - Ac3H11_663 0.68 -1.0 20,736 + Ac3H11_663 0.70 +0.2 20,736 + Ac3H11_663 0.70 -0.3 20,736 + Ac3H11_663 0.70 +0.7 20,736 + Ac3H11_663 0.70 +2.6 20,736 + Ac3H11_663 0.70 -1.3 20,736 + Ac3H11_663 0.70 +1.4 20,736 + Ac3H11_663 0.70 +1.0 20,736 + Ac3H11_663 0.70 -0.2 20,736 + Ac3H11_663 0.70 +0.1 20,736 + Ac3H11_663 0.70 +0.7 20,736 + Ac3H11_663 0.70 -0.7 20,736 + Ac3H11_663 0.70 +0.3 20,736 + Ac3H11_663 0.70 -2.0 20,736 + Ac3H11_663 0.70 -0.4 20,737 - Ac3H11_663 0.70 +0.1 20,737 - Ac3H11_663 0.70 +1.4 20,737 - Ac3H11_663 0.70 +0.8 20,737 - Ac3H11_663 0.70 -2.8 20,737 - Ac3H11_663 0.70 -3.5 20,737 - Ac3H11_663 0.70 -0.3 20,737 - Ac3H11_663 0.70 +0.7 20,737 - Ac3H11_663 0.70 +0.8 20,737 - Ac3H11_663 0.70 +0.9 20,877 + Ac3H11_663 0.78 +1.5 20,878 - Ac3H11_663 0.78 +0.2 20,976 + Ac3H11_663 0.84 +1.0 20,976 + Ac3H11_663 0.84 -1.4 21,240 + -0.5 21,241 - -1.2 21,378 + -1.5 21,379 - +0.6 21,379 - -0.1 21,385 - -0.6 21,385 - -0.6 21,385 - -1.5 21,385 - -0.2 21,661 + Ac3H11_664 0.37 +1.2 21,661 + Ac3H11_664 0.37 -0.3 21,661 + Ac3H11_664 0.37 -1.4 21,661 + Ac3H11_664 0.37 -0.6 21,661 + Ac3H11_664 0.37 +0.1 21,662 - Ac3H11_664 0.37 +0.3 21,662 - Ac3H11_664 0.37 -0.6 21,662 - Ac3H11_664 0.37 -2.5 21,662 - Ac3H11_664 0.37 +0.5 21,662 - Ac3H11_664 0.37 +0.0 21,662 - Ac3H11_664 0.37 +1.1 21,718 + Ac3H11_664 0.45 -1.8 21,719 - Ac3H11_664 0.45 -0.5 21,995 - Ac3H11_664 0.81 +0.9 22,211 + -0.0 22,211 + -1.5 22,212 - -0.3 22,212 - -1.5 22,212 - +0.8 22,224 - -0.6 22,224 - +0.2 22,338 + +0.4 22,338 + +1.6 22,338 + -0.4 22,339 - +0.2 22,339 - +1.0 22,339 - +0.5
Or see this region's nucleotide sequence