Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3780 and Ac3H11_3781 are separated by 118 nucleotides Ac3H11_3781 and Ac3H11_3782 are separated by 4 nucleotides Ac3H11_3782 and Ac3H11_3783 are separated by 43 nucleotides Ac3H11_3783 and Ac3H11_3784 are separated by 194 nucleotides Ac3H11_3784 and Ac3H11_3785 overlap by 13 nucleotides
Ac3H11_3780 - Phage (Mu-like) virion morphogenesis protein, at 28,630 to 29,907
_3780
Ac3H11_3781 - hypothetical protein, at 30,026 to 30,481
_3781
Ac3H11_3782 - hypothetical protein, at 30,486 to 30,686
_3782
Ac3H11_3783 - Phage protein, at 30,730 to 31,311
_3783
Ac3H11_3784 - hypothetical protein, at 31,506 to 31,637
_3784
Ac3H11_3785 - Nucleoside-diphosphate-sugar epimerases, at 31,625 to 32,608
_3785
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 29.833 kb on + strand at 29.834 kb on - strand at 29.834 kb on - strand at 29.953 kb on + strand at 30.072 kb on - strand, within Ac3H11_3781 at 30.228 kb on - strand, within Ac3H11_3781 at 30.456 kb on + strand at 30.456 kb on + strand at 30.457 kb on - strand at 30.538 kb on - strand, within Ac3H11_3782 at 30.595 kb on - strand, within Ac3H11_3782 at 30.603 kb on + strand, within Ac3H11_3782 at 30.603 kb on + strand, within Ac3H11_3782 at 30.603 kb on + strand, within Ac3H11_3782 at 30.604 kb on - strand, within Ac3H11_3782 at 30.604 kb on - strand, within Ac3H11_3782 at 30.615 kb on + strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.616 kb on - strand, within Ac3H11_3782 at 30.679 kb on - strand at 30.742 kb on + strand at 30.857 kb on + strand, within Ac3H11_3783 at 30.858 kb on - strand, within Ac3H11_3783 at 31.082 kb on - strand, within Ac3H11_3783 at 31.082 kb on - strand, within Ac3H11_3783 at 31.082 kb on - strand, within Ac3H11_3783 at 31.111 kb on - strand, within Ac3H11_3783 at 31.207 kb on + strand, within Ac3H11_3783 at 31.207 kb on + strand, within Ac3H11_3783 at 31.207 kb on + strand, within Ac3H11_3783 at 31.207 kb on + strand, within Ac3H11_3783 at 31.207 kb on + strand, within Ac3H11_3783 at 31.207 kb on + strand, within Ac3H11_3783 at 31.208 kb on - strand, within Ac3H11_3783 at 31.208 kb on - strand, within Ac3H11_3783 at 31.208 kb on - strand, within Ac3H11_3783 at 31.283 kb on + strand at 31.284 kb on - strand at 31.284 kb on - strand at 31.369 kb on - strand at 31.369 kb on - strand at 31.444 kb on + strand at 31.445 kb on - strand at 31.445 kb on - strand at 31.445 kb on - strand at 31.467 kb on + strand at 31.467 kb on + strand at 31.626 kb on + strand at 31.649 kb on + strand at 31.650 kb on - strand at 31.834 kb on - strand, within Ac3H11_3785 at 31.857 kb on + strand, within Ac3H11_3785 at 31.858 kb on - strand, within Ac3H11_3785 at 31.858 kb on - strand, within Ac3H11_3785 at 32.055 kb on + strand, within Ac3H11_3785 at 32.056 kb on - strand, within Ac3H11_3785 at 32.056 kb on - strand, within Ac3H11_3785 at 32.056 kb on - strand, within Ac3H11_3785 at 32.056 kb on - strand, within Ac3H11_3785 at 32.056 kb on - strand, within Ac3H11_3785 at 32.056 kb on - strand, within Ac3H11_3785 at 32.205 kb on + strand, within Ac3H11_3785 at 32.206 kb on - strand, within Ac3H11_3785 at 32.206 kb on - strand, within Ac3H11_3785 at 32.206 kb on - strand, within Ac3H11_3785 at 32.227 kb on - strand, within Ac3H11_3785 at 32.227 kb on - strand, within Ac3H11_3785 at 32.227 kb on - strand, within Ac3H11_3785
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 29,833 + +1.5 29,834 - -2.7 29,834 - +1.1 29,953 + -1.3 30,072 - Ac3H11_3781 0.10 +0.8 30,228 - Ac3H11_3781 0.44 -3.3 30,456 + +1.1 30,456 + -0.2 30,457 - +1.2 30,538 - Ac3H11_3782 0.26 -0.9 30,595 - Ac3H11_3782 0.54 -0.6 30,603 + Ac3H11_3782 0.58 -1.9 30,603 + Ac3H11_3782 0.58 -1.6 30,603 + Ac3H11_3782 0.58 +0.3 30,604 - Ac3H11_3782 0.59 -0.0 30,604 - Ac3H11_3782 0.59 -0.4 30,615 + Ac3H11_3782 0.64 -3.5 30,616 - Ac3H11_3782 0.65 -0.6 30,616 - Ac3H11_3782 0.65 +0.9 30,616 - Ac3H11_3782 0.65 +0.1 30,616 - Ac3H11_3782 0.65 +0.0 30,616 - Ac3H11_3782 0.65 -0.9 30,616 - Ac3H11_3782 0.65 -1.1 30,679 - +1.3 30,742 + +1.2 30,857 + Ac3H11_3783 0.22 +0.7 30,858 - Ac3H11_3783 0.22 -0.7 31,082 - Ac3H11_3783 0.60 -1.8 31,082 - Ac3H11_3783 0.60 +0.5 31,082 - Ac3H11_3783 0.60 +0.4 31,111 - Ac3H11_3783 0.65 -2.7 31,207 + Ac3H11_3783 0.82 -0.6 31,207 + Ac3H11_3783 0.82 +0.1 31,207 + Ac3H11_3783 0.82 +0.9 31,207 + Ac3H11_3783 0.82 -0.8 31,207 + Ac3H11_3783 0.82 -0.5 31,207 + Ac3H11_3783 0.82 +0.3 31,208 - Ac3H11_3783 0.82 +0.5 31,208 - Ac3H11_3783 0.82 -0.4 31,208 - Ac3H11_3783 0.82 +0.6 31,283 + -0.1 31,284 - -0.2 31,284 - -1.5 31,369 - -2.2 31,369 - -0.1 31,444 + +0.9 31,445 - +0.1 31,445 - -0.7 31,445 - -0.6 31,467 + -0.8 31,467 + +1.2 31,626 + +2.2 31,649 + +1.1 31,650 - +1.1 31,834 - Ac3H11_3785 0.21 +0.2 31,857 + Ac3H11_3785 0.24 -0.8 31,858 - Ac3H11_3785 0.24 +0.3 31,858 - Ac3H11_3785 0.24 -0.5 32,055 + Ac3H11_3785 0.44 +0.0 32,056 - Ac3H11_3785 0.44 +0.2 32,056 - Ac3H11_3785 0.44 -3.1 32,056 - Ac3H11_3785 0.44 -1.3 32,056 - Ac3H11_3785 0.44 +0.8 32,056 - Ac3H11_3785 0.44 -0.7 32,056 - Ac3H11_3785 0.44 -0.3 32,205 + Ac3H11_3785 0.59 +0.1 32,206 - Ac3H11_3785 0.59 -0.7 32,206 - Ac3H11_3785 0.59 -0.4 32,206 - Ac3H11_3785 0.59 +1.1 32,227 - Ac3H11_3785 0.61 +0.4 32,227 - Ac3H11_3785 0.61 +0.3 32,227 - Ac3H11_3785 0.61 +0.4
Or see this region's nucleotide sequence