Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_342 and Ac3H11_343 are separated by 11 nucleotides Ac3H11_343 and Ac3H11_344 are separated by 88 nucleotides Ac3H11_344 and Ac3H11_345 are separated by 228 nucleotides Ac3H11_345 and Ac3H11_346 overlap by 4 nucleotides
Ac3H11_342 - Osmolarity sensory histidine kinase EnvZ, at 19,071 to 20,594
_342
Ac3H11_343 - Two-component system response regulator OmpR, at 20,606 to 21,337
_343
Ac3H11_344 - hypothetical protein, at 21,426 to 21,599
_344
Ac3H11_345 - hypothetical protein, at 21,828 to 22,223
_345
Ac3H11_346 - hypothetical protein, at 22,220 to 22,435
_346
Position (kb) 20
21
22 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 20.591 kb on - strand at 21.428 kb on - strand at 21.810 kb on - strand at 22.033 kb on - strand, within Ac3H11_345 at 22.059 kb on + strand, within Ac3H11_345 at 22.060 kb on - strand, within Ac3H11_345 at 22.325 kb on + strand, within Ac3H11_346 at 22.326 kb on - strand, within Ac3H11_346
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 20,591 - -1.4 20,591 - +2.0 20,591 - +3.0 20,591 - +1.1 20,591 - -1.7 20,591 - +2.7 20,591 - +1.4 20,591 - +3.0 20,591 - +2.4 20,591 - -0.1 21,428 - -0.4 21,810 - -0.4 22,033 - Ac3H11_345 0.52 -0.4 22,059 + Ac3H11_345 0.58 -2.0 22,060 - Ac3H11_345 0.59 -0.2 22,325 + Ac3H11_346 0.49 -2.5 22,326 - Ac3H11_346 0.49 +0.1
Or see this region's nucleotide sequence