Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3011 and Ac3H11_3012 are separated by 15 nucleotides Ac3H11_3012 and Ac3H11_3013 are separated by 26 nucleotides Ac3H11_3013 and Ac3H11_3014 overlap by 4 nucleotides Ac3H11_3014 and Ac3H11_3015 are separated by 20 nucleotides
Ac3H11_3011 - Dienelactone hydrolase and related enzymes, at 33,410 to 34,297
_3011
Ac3H11_3012 - dienelactone hydrolase, at 34,313 to 35,188
_3012
Ac3H11_3013 - DinB family protein, at 35,215 to 35,700
_3013
Ac3H11_3014 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18), at 35,697 to 36,482
_3014
Ac3H11_3015 - Probable transmembrane protein, at 36,503 to 37,228
_3015
Position (kb) 35
36 Strain fitness (log2 ratio) -1
0
1
2 at 34.295 kb on + strand at 34.508 kb on + strand, within Ac3H11_3012 at 34.508 kb on + strand, within Ac3H11_3012 at 34.574 kb on + strand, within Ac3H11_3012 at 34.574 kb on + strand, within Ac3H11_3012 at 35.009 kb on + strand, within Ac3H11_3012 at 35.009 kb on + strand, within Ac3H11_3012 at 35.021 kb on + strand, within Ac3H11_3012 at 35.021 kb on + strand, within Ac3H11_3012 at 35.021 kb on + strand, within Ac3H11_3012 at 35.190 kb on + strand at 35.190 kb on + strand at 35.190 kb on + strand at 35.227 kb on + strand at 35.402 kb on + strand, within Ac3H11_3013 at 35.527 kb on + strand, within Ac3H11_3013 at 35.527 kb on + strand, within Ac3H11_3013 at 35.527 kb on + strand, within Ac3H11_3013 at 35.527 kb on + strand, within Ac3H11_3013 at 35.671 kb on + strand at 35.672 kb on - strand at 36.495 kb on + strand at 36.495 kb on + strand at 36.495 kb on + strand at 36.495 kb on + strand at 36.495 kb on + strand at 36.495 kb on + strand at 36.495 kb on + strand at 36.496 kb on - strand at 36.496 kb on - strand at 36.496 kb on - strand at 36.556 kb on + strand at 36.556 kb on + strand at 36.556 kb on + strand at 36.677 kb on + strand, within Ac3H11_3015
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 34,295 + -1.0 34,508 + Ac3H11_3012 0.22 +1.4 34,508 + Ac3H11_3012 0.22 -0.6 34,574 + Ac3H11_3012 0.30 -0.8 34,574 + Ac3H11_3012 0.30 +0.8 35,009 + Ac3H11_3012 0.79 -0.8 35,009 + Ac3H11_3012 0.79 +1.4 35,021 + Ac3H11_3012 0.81 -0.4 35,021 + Ac3H11_3012 0.81 -0.4 35,021 + Ac3H11_3012 0.81 -0.3 35,190 + +0.2 35,190 + +1.2 35,190 + +0.0 35,227 + -1.2 35,402 + Ac3H11_3013 0.38 -0.1 35,527 + Ac3H11_3013 0.64 +0.0 35,527 + Ac3H11_3013 0.64 +0.2 35,527 + Ac3H11_3013 0.64 -0.3 35,527 + Ac3H11_3013 0.64 -0.8 35,671 + +1.5 35,672 - +1.3 36,495 + -0.2 36,495 + -0.1 36,495 + +0.5 36,495 + -0.1 36,495 + -0.1 36,495 + +0.5 36,495 + +1.6 36,496 - +0.5 36,496 - +2.1 36,496 - +1.1 36,556 + -0.2 36,556 + +1.0 36,556 + +0.6 36,677 + Ac3H11_3015 0.24 +1.0
Or see this region's nucleotide sequence