Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2928 and Ac3H11_2929 are separated by 2 nucleotides Ac3H11_2929 and Ac3H11_2930 are separated by 30 nucleotides Ac3H11_2930 and Ac3H11_2931 are separated by 192 nucleotides Ac3H11_2931 and Ac3H11_2932 are separated by 90 nucleotides
Ac3H11_2928 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48), at 1,245,667 to 1,246,977
_2928
Ac3H11_2929 - Probable hydrolase protein (EC 3.-.-.-), at 1,246,980 to 1,247,798
_2929
Ac3H11_2930 - putative membrane protein, at 1,247,829 to 1,249,085
_2930
Ac3H11_2931 - putative transmembrane protein, at 1,249,278 to 1,249,613
_2931
Ac3H11_2932 - Ribonuclease BN (EC 3.1.-.-), at 1,249,704 to 1,250,966
_2932
Position (kb)
1247
1248
1249
1250 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1247.010 kb on + strand at 1247.010 kb on + strand at 1247.010 kb on + strand at 1247.010 kb on + strand at 1247.010 kb on + strand at 1247.010 kb on + strand at 1247.010 kb on + strand at 1247.010 kb on + strand at 1247.010 kb on + strand at 1247.010 kb on + strand at 1247.010 kb on + strand at 1247.010 kb on + strand at 1247.011 kb on - strand at 1247.011 kb on - strand at 1247.011 kb on - strand at 1247.011 kb on - strand at 1247.011 kb on - strand at 1247.011 kb on - strand at 1247.011 kb on - strand at 1247.011 kb on - strand at 1247.016 kb on + strand at 1247.016 kb on + strand at 1247.112 kb on + strand, within Ac3H11_2929 at 1247.112 kb on + strand, within Ac3H11_2929 at 1247.113 kb on - strand, within Ac3H11_2929 at 1247.113 kb on - strand, within Ac3H11_2929 at 1247.574 kb on + strand, within Ac3H11_2929 at 1247.574 kb on + strand, within Ac3H11_2929 at 1247.575 kb on - strand, within Ac3H11_2929 at 1247.575 kb on - strand, within Ac3H11_2929 at 1247.575 kb on - strand, within Ac3H11_2929 at 1247.903 kb on - strand at 1247.950 kb on + strand at 1247.950 kb on + strand at 1247.951 kb on - strand at 1248.013 kb on + strand, within Ac3H11_2930 at 1248.013 kb on + strand, within Ac3H11_2930 at 1248.013 kb on + strand, within Ac3H11_2930 at 1248.055 kb on + strand, within Ac3H11_2930 at 1248.055 kb on + strand, within Ac3H11_2930 at 1248.056 kb on - strand, within Ac3H11_2930 at 1248.056 kb on - strand, within Ac3H11_2930 at 1248.151 kb on + strand, within Ac3H11_2930 at 1248.256 kb on + strand, within Ac3H11_2930 at 1248.256 kb on + strand, within Ac3H11_2930 at 1248.257 kb on - strand, within Ac3H11_2930 at 1248.481 kb on + strand, within Ac3H11_2930 at 1248.673 kb on + strand, within Ac3H11_2930 at 1248.673 kb on + strand, within Ac3H11_2930 at 1248.674 kb on - strand, within Ac3H11_2930 at 1248.674 kb on - strand, within Ac3H11_2930 at 1248.674 kb on - strand, within Ac3H11_2930 at 1248.842 kb on - strand, within Ac3H11_2930 at 1248.842 kb on - strand, within Ac3H11_2930 at 1248.934 kb on + strand, within Ac3H11_2930 at 1248.934 kb on + strand, within Ac3H11_2930 at 1248.935 kb on - strand, within Ac3H11_2930 at 1248.935 kb on - strand, within Ac3H11_2930 at 1248.935 kb on - strand, within Ac3H11_2930 at 1248.935 kb on - strand, within Ac3H11_2930 at 1249.162 kb on + strand at 1249.163 kb on - strand at 1249.163 kb on - strand at 1249.163 kb on - strand at 1249.164 kb on + strand at 1249.164 kb on + strand at 1249.164 kb on + strand at 1249.164 kb on + strand at 1249.165 kb on - strand at 1249.243 kb on + strand at 1249.243 kb on + strand at 1249.243 kb on + strand at 1249.244 kb on - strand at 1249.244 kb on - strand at 1249.244 kb on - strand at 1249.244 kb on - strand at 1249.244 kb on - strand at 1249.244 kb on - strand at 1249.244 kb on - strand at 1249.341 kb on + strand, within Ac3H11_2931 at 1249.342 kb on - strand, within Ac3H11_2931 at 1249.350 kb on + strand, within Ac3H11_2931 at 1249.350 kb on + strand, within Ac3H11_2931 at 1249.350 kb on + strand, within Ac3H11_2931 at 1249.350 kb on + strand, within Ac3H11_2931 at 1249.351 kb on - strand, within Ac3H11_2931 at 1249.351 kb on - strand, within Ac3H11_2931 at 1249.351 kb on - strand, within Ac3H11_2931 at 1249.351 kb on - strand, within Ac3H11_2931 at 1249.351 kb on - strand, within Ac3H11_2931 at 1249.351 kb on - strand, within Ac3H11_2931 at 1249.351 kb on - strand, within Ac3H11_2931 at 1249.456 kb on - strand, within Ac3H11_2931 at 1249.464 kb on + strand, within Ac3H11_2931 at 1249.465 kb on - strand, within Ac3H11_2931 at 1249.465 kb on - strand, within Ac3H11_2931 at 1249.465 kb on - strand, within Ac3H11_2931 at 1249.551 kb on + strand, within Ac3H11_2931 at 1249.861 kb on + strand, within Ac3H11_2932
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 1,247,010 + +0.3 1,247,010 + -0.6 1,247,010 + +1.0 1,247,010 + +1.1 1,247,010 + -0.3 1,247,010 + +0.4 1,247,010 + -2.7 1,247,010 + +0.1 1,247,010 + -0.3 1,247,010 + -0.0 1,247,010 + +0.6 1,247,010 + +1.2 1,247,011 - -0.9 1,247,011 - +0.2 1,247,011 - +0.1 1,247,011 - -2.2 1,247,011 - -0.1 1,247,011 - -0.8 1,247,011 - -0.2 1,247,011 - +1.0 1,247,016 + -1.5 1,247,016 + +0.2 1,247,112 + Ac3H11_2929 0.16 +0.1 1,247,112 + Ac3H11_2929 0.16 -0.1 1,247,113 - Ac3H11_2929 0.16 +1.6 1,247,113 - Ac3H11_2929 0.16 +1.3 1,247,574 + Ac3H11_2929 0.73 +0.8 1,247,574 + Ac3H11_2929 0.73 +0.8 1,247,575 - Ac3H11_2929 0.73 +1.1 1,247,575 - Ac3H11_2929 0.73 -1.1 1,247,575 - Ac3H11_2929 0.73 -0.7 1,247,903 - -0.5 1,247,950 + +1.6 1,247,950 + -0.9 1,247,951 - +0.6 1,248,013 + Ac3H11_2930 0.15 +0.3 1,248,013 + Ac3H11_2930 0.15 +1.1 1,248,013 + Ac3H11_2930 0.15 -0.3 1,248,055 + Ac3H11_2930 0.18 -1.9 1,248,055 + Ac3H11_2930 0.18 +0.5 1,248,056 - Ac3H11_2930 0.18 -0.1 1,248,056 - Ac3H11_2930 0.18 -0.4 1,248,151 + Ac3H11_2930 0.26 +0.3 1,248,256 + Ac3H11_2930 0.34 -2.1 1,248,256 + Ac3H11_2930 0.34 +0.2 1,248,257 - Ac3H11_2930 0.34 +2.1 1,248,481 + Ac3H11_2930 0.52 +0.6 1,248,673 + Ac3H11_2930 0.67 +1.2 1,248,673 + Ac3H11_2930 0.67 +0.5 1,248,674 - Ac3H11_2930 0.67 -1.0 1,248,674 - Ac3H11_2930 0.67 -1.1 1,248,674 - Ac3H11_2930 0.67 +0.5 1,248,842 - Ac3H11_2930 0.81 -0.7 1,248,842 - Ac3H11_2930 0.81 -0.1 1,248,934 + Ac3H11_2930 0.88 -1.4 1,248,934 + Ac3H11_2930 0.88 -0.9 1,248,935 - Ac3H11_2930 0.88 -2.3 1,248,935 - Ac3H11_2930 0.88 +0.1 1,248,935 - Ac3H11_2930 0.88 -1.1 1,248,935 - Ac3H11_2930 0.88 -1.2 1,249,162 + +0.8 1,249,163 - -2.5 1,249,163 - -0.9 1,249,163 - -2.1 1,249,164 + +1.1 1,249,164 + +0.5 1,249,164 + -1.1 1,249,164 + +1.5 1,249,165 - -0.0 1,249,243 + +1.8 1,249,243 + +0.2 1,249,243 + -2.7 1,249,244 - -0.1 1,249,244 - -0.8 1,249,244 - -2.7 1,249,244 - -1.0 1,249,244 - -0.4 1,249,244 - +0.8 1,249,244 - -0.3 1,249,341 + Ac3H11_2931 0.19 -1.0 1,249,342 - Ac3H11_2931 0.19 +0.3 1,249,350 + Ac3H11_2931 0.21 +0.5 1,249,350 + Ac3H11_2931 0.21 -1.5 1,249,350 + Ac3H11_2931 0.21 -3.0 1,249,350 + Ac3H11_2931 0.21 -2.5 1,249,351 - Ac3H11_2931 0.22 +1.1 1,249,351 - Ac3H11_2931 0.22 +0.2 1,249,351 - Ac3H11_2931 0.22 -2.1 1,249,351 - Ac3H11_2931 0.22 -0.1 1,249,351 - Ac3H11_2931 0.22 +1.9 1,249,351 - Ac3H11_2931 0.22 +0.4 1,249,351 - Ac3H11_2931 0.22 -1.3 1,249,456 - Ac3H11_2931 0.53 +0.4 1,249,464 + Ac3H11_2931 0.55 +0.0 1,249,465 - Ac3H11_2931 0.56 +3.2 1,249,465 - Ac3H11_2931 0.56 +3.1 1,249,465 - Ac3H11_2931 0.56 -1.5 1,249,551 + Ac3H11_2931 0.81 -0.1 1,249,861 + Ac3H11_2932 0.12 +1.5
Or see this region's nucleotide sequence