Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2733 and Ac3H11_2734 overlap by 7 nucleotides Ac3H11_2734 and Ac3H11_2735 are separated by 1 nucleotides Ac3H11_2735 and Ac3H11_2736 are separated by 111 nucleotides
Ac3H11_2733 - MFS transporter, at 1,033,840 to 1,035,060
_2733
Ac3H11_2734 - Dihydroneopterin aldolase (EC 4.1.2.25), at 1,035,054 to 1,035,482
_2734
Ac3H11_2735 - FolM Alternative dihydrofolate reductase 1, at 1,035,484 to 1,036,209
_2735
Ac3H11_2736 - Glutathione S-transferase (EC 2.5.1.18), at 1,036,321 to 1,036,899
_2736
Position (kb)
1035
1036 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1034.396 kb on + strand, within Ac3H11_2733 at 1034.409 kb on - strand, within Ac3H11_2733 at 1034.620 kb on + strand, within Ac3H11_2733 at 1034.621 kb on - strand, within Ac3H11_2733 at 1034.621 kb on - strand, within Ac3H11_2733 at 1034.621 kb on - strand, within Ac3H11_2733 at 1034.897 kb on - strand, within Ac3H11_2733 at 1035.011 kb on + strand at 1035.012 kb on - strand at 1035.139 kb on + strand, within Ac3H11_2734 at 1035.178 kb on - strand, within Ac3H11_2734 at 1035.178 kb on - strand, within Ac3H11_2734 at 1035.178 kb on - strand, within Ac3H11_2734 at 1035.237 kb on + strand, within Ac3H11_2734 at 1035.237 kb on + strand, within Ac3H11_2734 at 1035.237 kb on + strand, within Ac3H11_2734 at 1035.237 kb on + strand, within Ac3H11_2734 at 1035.237 kb on + strand, within Ac3H11_2734 at 1035.237 kb on + strand, within Ac3H11_2734 at 1035.238 kb on - strand, within Ac3H11_2734 at 1035.238 kb on - strand, within Ac3H11_2734 at 1035.239 kb on + strand, within Ac3H11_2734 at 1035.239 kb on + strand, within Ac3H11_2734 at 1035.239 kb on + strand, within Ac3H11_2734 at 1035.239 kb on + strand, within Ac3H11_2734 at 1035.239 kb on + strand, within Ac3H11_2734 at 1035.239 kb on + strand, within Ac3H11_2734 at 1035.240 kb on - strand, within Ac3H11_2734 at 1035.240 kb on - strand, within Ac3H11_2734 at 1035.240 kb on - strand, within Ac3H11_2734 at 1035.240 kb on - strand, within Ac3H11_2734 at 1035.240 kb on - strand, within Ac3H11_2734 at 1035.343 kb on + strand, within Ac3H11_2734 at 1035.344 kb on - strand, within Ac3H11_2734 at 1035.344 kb on - strand, within Ac3H11_2734 at 1035.360 kb on - strand, within Ac3H11_2734 at 1035.360 kb on - strand, within Ac3H11_2734 at 1035.360 kb on - strand, within Ac3H11_2734 at 1035.728 kb on - strand, within Ac3H11_2735 at 1035.801 kb on - strand, within Ac3H11_2735 at 1035.890 kb on + strand, within Ac3H11_2735 at 1035.890 kb on + strand, within Ac3H11_2735 at 1035.890 kb on + strand, within Ac3H11_2735 at 1035.890 kb on + strand, within Ac3H11_2735 at 1035.891 kb on - strand, within Ac3H11_2735 at 1035.891 kb on - strand, within Ac3H11_2735 at 1035.892 kb on + strand, within Ac3H11_2735 at 1035.893 kb on - strand, within Ac3H11_2735 at 1035.893 kb on - strand, within Ac3H11_2735 at 1036.376 kb on + strand at 1036.376 kb on + strand at 1036.475 kb on + strand, within Ac3H11_2736 at 1036.476 kb on - strand, within Ac3H11_2736
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 1,034,396 + Ac3H11_2733 0.46 +1.9 1,034,409 - Ac3H11_2733 0.47 -0.9 1,034,620 + Ac3H11_2733 0.64 -0.6 1,034,621 - Ac3H11_2733 0.64 +0.6 1,034,621 - Ac3H11_2733 0.64 -0.1 1,034,621 - Ac3H11_2733 0.64 -0.2 1,034,897 - Ac3H11_2733 0.87 -0.3 1,035,011 + +0.3 1,035,012 - -2.4 1,035,139 + Ac3H11_2734 0.20 +1.3 1,035,178 - Ac3H11_2734 0.29 +0.3 1,035,178 - Ac3H11_2734 0.29 +1.2 1,035,178 - Ac3H11_2734 0.29 +0.8 1,035,237 + Ac3H11_2734 0.43 +0.6 1,035,237 + Ac3H11_2734 0.43 -1.4 1,035,237 + Ac3H11_2734 0.43 -1.3 1,035,237 + Ac3H11_2734 0.43 +1.4 1,035,237 + Ac3H11_2734 0.43 +0.3 1,035,237 + Ac3H11_2734 0.43 -1.8 1,035,238 - Ac3H11_2734 0.43 +0.5 1,035,238 - Ac3H11_2734 0.43 +1.0 1,035,239 + Ac3H11_2734 0.43 -0.5 1,035,239 + Ac3H11_2734 0.43 -0.5 1,035,239 + Ac3H11_2734 0.43 -0.2 1,035,239 + Ac3H11_2734 0.43 +1.2 1,035,239 + Ac3H11_2734 0.43 -1.2 1,035,239 + Ac3H11_2734 0.43 -0.3 1,035,240 - Ac3H11_2734 0.43 -0.6 1,035,240 - Ac3H11_2734 0.43 -1.3 1,035,240 - Ac3H11_2734 0.43 +0.7 1,035,240 - Ac3H11_2734 0.43 +1.2 1,035,240 - Ac3H11_2734 0.43 +0.0 1,035,343 + Ac3H11_2734 0.67 -2.3 1,035,344 - Ac3H11_2734 0.68 -1.0 1,035,344 - Ac3H11_2734 0.68 +1.6 1,035,360 - Ac3H11_2734 0.71 +1.7 1,035,360 - Ac3H11_2734 0.71 -0.5 1,035,360 - Ac3H11_2734 0.71 -2.3 1,035,728 - Ac3H11_2735 0.34 -2.9 1,035,801 - Ac3H11_2735 0.44 +0.5 1,035,890 + Ac3H11_2735 0.56 -2.1 1,035,890 + Ac3H11_2735 0.56 -0.5 1,035,890 + Ac3H11_2735 0.56 -0.3 1,035,890 + Ac3H11_2735 0.56 +0.2 1,035,891 - Ac3H11_2735 0.56 -1.0 1,035,891 - Ac3H11_2735 0.56 +0.2 1,035,892 + Ac3H11_2735 0.56 -0.1 1,035,893 - Ac3H11_2735 0.56 -0.1 1,035,893 - Ac3H11_2735 0.56 +1.1 1,036,376 + -1.1 1,036,376 + +0.0 1,036,475 + Ac3H11_2736 0.27 -0.7 1,036,476 - Ac3H11_2736 0.27 -0.2
Or see this region's nucleotide sequence