Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2522 and Ac3H11_2523 are separated by 40 nucleotides Ac3H11_2523 and Ac3H11_2524 are separated by 190 nucleotides Ac3H11_2524 and Ac3H11_2525 are separated by 1 nucleotides
Ac3H11_2522 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 798,397 to 799,572
_2522
Ac3H11_2523 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 799,613 to 801,712
_2523
Ac3H11_2524 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 801,903 to 802,598
_2524
Ac3H11_2525 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 802,600 to 803,385
_2525
Position (kb) 799
800
801
802 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 798.789 kb on - strand, within Ac3H11_2522 at 798.789 kb on - strand, within Ac3H11_2522 at 798.789 kb on - strand, within Ac3H11_2522 at 798.891 kb on + strand, within Ac3H11_2522 at 798.891 kb on + strand, within Ac3H11_2522 at 798.892 kb on - strand, within Ac3H11_2522 at 798.892 kb on - strand, within Ac3H11_2522 at 798.892 kb on - strand, within Ac3H11_2522 at 798.892 kb on - strand, within Ac3H11_2522 at 799.067 kb on + strand, within Ac3H11_2522 at 799.067 kb on + strand, within Ac3H11_2522 at 799.067 kb on + strand, within Ac3H11_2522 at 799.097 kb on + strand, within Ac3H11_2522 at 799.097 kb on + strand, within Ac3H11_2522 at 799.097 kb on + strand, within Ac3H11_2522 at 799.098 kb on - strand, within Ac3H11_2522 at 799.098 kb on - strand, within Ac3H11_2522 at 799.140 kb on - strand, within Ac3H11_2522 at 799.140 kb on - strand, within Ac3H11_2522 at 799.140 kb on - strand, within Ac3H11_2522 at 799.443 kb on + strand, within Ac3H11_2522 at 799.456 kb on + strand at 799.457 kb on - strand at 799.592 kb on + strand at 799.592 kb on + strand at 799.592 kb on + strand at 799.610 kb on + strand at 799.610 kb on + strand at 799.611 kb on - strand at 799.611 kb on - strand at 799.614 kb on + strand at 799.614 kb on + strand at 799.615 kb on - strand at 799.615 kb on - strand at 799.615 kb on - strand at 799.615 kb on - strand at 799.615 kb on - strand at 799.615 kb on - strand at 799.749 kb on + strand at 799.749 kb on + strand at 799.749 kb on + strand at 799.750 kb on - strand at 799.797 kb on + strand at 799.797 kb on + strand at 799.797 kb on + strand at 799.798 kb on - strand at 799.798 kb on - strand at 799.798 kb on - strand at 799.798 kb on - strand at 799.798 kb on - strand at 799.798 kb on - strand at 799.999 kb on - strand, within Ac3H11_2523 at 799.999 kb on - strand, within Ac3H11_2523 at 800.070 kb on + strand, within Ac3H11_2523 at 800.070 kb on + strand, within Ac3H11_2523 at 800.071 kb on - strand, within Ac3H11_2523 at 800.244 kb on + strand, within Ac3H11_2523 at 800.244 kb on + strand, within Ac3H11_2523 at 800.244 kb on + strand, within Ac3H11_2523 at 800.244 kb on + strand, within Ac3H11_2523 at 800.244 kb on + strand, within Ac3H11_2523 at 800.245 kb on - strand, within Ac3H11_2523 at 800.473 kb on + strand, within Ac3H11_2523 at 800.473 kb on + strand, within Ac3H11_2523 at 800.474 kb on - strand, within Ac3H11_2523 at 800.474 kb on - strand, within Ac3H11_2523 at 800.474 kb on - strand, within Ac3H11_2523 at 800.474 kb on - strand, within Ac3H11_2523 at 800.474 kb on - strand, within Ac3H11_2523 at 800.474 kb on - strand, within Ac3H11_2523 at 800.640 kb on + strand, within Ac3H11_2523 at 800.640 kb on + strand, within Ac3H11_2523 at 800.640 kb on + strand, within Ac3H11_2523 at 800.640 kb on + strand, within Ac3H11_2523 at 800.640 kb on + strand, within Ac3H11_2523 at 800.641 kb on - strand, within Ac3H11_2523 at 800.641 kb on - strand, within Ac3H11_2523 at 800.641 kb on - strand, within Ac3H11_2523 at 800.641 kb on - strand, within Ac3H11_2523 at 800.641 kb on - strand, within Ac3H11_2523 at 800.641 kb on - strand, within Ac3H11_2523 at 800.682 kb on + strand, within Ac3H11_2523 at 800.682 kb on + strand, within Ac3H11_2523 at 800.683 kb on - strand, within Ac3H11_2523 at 801.066 kb on + strand, within Ac3H11_2523 at 801.066 kb on + strand, within Ac3H11_2523 at 801.066 kb on + strand, within Ac3H11_2523 at 801.067 kb on - strand, within Ac3H11_2523 at 801.067 kb on - strand, within Ac3H11_2523 at 801.067 kb on - strand, within Ac3H11_2523 at 801.067 kb on - strand, within Ac3H11_2523 at 801.067 kb on - strand, within Ac3H11_2523 at 801.067 kb on - strand, within Ac3H11_2523 at 801.067 kb on - strand, within Ac3H11_2523 at 801.379 kb on - strand, within Ac3H11_2523 at 801.699 kb on + strand at 801.699 kb on + strand at 801.699 kb on + strand at 801.699 kb on + strand at 801.700 kb on - strand at 801.700 kb on - strand at 801.700 kb on - strand at 801.764 kb on + strand at 801.764 kb on + strand at 801.764 kb on + strand at 801.838 kb on - strand at 801.865 kb on - strand at 801.931 kb on + strand at 801.931 kb on + strand at 801.931 kb on + strand at 801.931 kb on + strand at 801.931 kb on + strand at 801.931 kb on + strand at 801.931 kb on + strand at 801.931 kb on + strand at 801.931 kb on + strand at 801.931 kb on + strand at 801.932 kb on - strand at 801.932 kb on - strand at 801.932 kb on - strand at 801.932 kb on - strand at 801.932 kb on - strand at 801.932 kb on - strand at 801.932 kb on - strand at 801.932 kb on - strand at 801.932 kb on - strand at 801.932 kb on - strand at 801.935 kb on + strand at 801.935 kb on + strand at 801.935 kb on + strand at 801.935 kb on + strand at 801.935 kb on + strand at 801.935 kb on + strand at 801.935 kb on + strand at 801.935 kb on + strand at 801.936 kb on - strand at 802.081 kb on + strand at 802.082 kb on - strand, within Ac3H11_2524 at 802.255 kb on + strand, within Ac3H11_2524 at 802.255 kb on + strand, within Ac3H11_2524 at 802.255 kb on + strand, within Ac3H11_2524 at 802.255 kb on + strand, within Ac3H11_2524 at 802.255 kb on + strand, within Ac3H11_2524 at 802.255 kb on + strand, within Ac3H11_2524 at 802.255 kb on + strand, within Ac3H11_2524 at 802.256 kb on - strand, within Ac3H11_2524 at 802.256 kb on - strand, within Ac3H11_2524 at 802.256 kb on - strand, within Ac3H11_2524 at 802.364 kb on - strand, within Ac3H11_2524 at 802.364 kb on - strand, within Ac3H11_2524 at 802.567 kb on + strand at 802.570 kb on + strand at 802.570 kb on + strand at 802.570 kb on + strand at 802.571 kb on - strand at 802.571 kb on - strand at 802.643 kb on + strand at 802.643 kb on + strand at 802.644 kb on - strand at 802.709 kb on + strand, within Ac3H11_2525 at 802.710 kb on - strand, within Ac3H11_2525 at 802.710 kb on - strand, within Ac3H11_2525 at 802.710 kb on - strand, within Ac3H11_2525
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 798,789 - Ac3H11_2522 0.33 -2.4 798,789 - Ac3H11_2522 0.33 -0.9 798,789 - Ac3H11_2522 0.33 +0.0 798,891 + Ac3H11_2522 0.42 -0.9 798,891 + Ac3H11_2522 0.42 +0.6 798,892 - Ac3H11_2522 0.42 -1.2 798,892 - Ac3H11_2522 0.42 -0.3 798,892 - Ac3H11_2522 0.42 +0.7 798,892 - Ac3H11_2522 0.42 -0.1 799,067 + Ac3H11_2522 0.57 +0.4 799,067 + Ac3H11_2522 0.57 +0.7 799,067 + Ac3H11_2522 0.57 -1.5 799,097 + Ac3H11_2522 0.60 +1.4 799,097 + Ac3H11_2522 0.60 -0.6 799,097 + Ac3H11_2522 0.60 +1.1 799,098 - Ac3H11_2522 0.60 -2.4 799,098 - Ac3H11_2522 0.60 +1.0 799,140 - Ac3H11_2522 0.63 +0.9 799,140 - Ac3H11_2522 0.63 +0.1 799,140 - Ac3H11_2522 0.63 -2.0 799,443 + Ac3H11_2522 0.89 +0.9 799,456 + +0.8 799,457 - +0.6 799,592 + -3.1 799,592 + -0.7 799,592 + +0.8 799,610 + +0.2 799,610 + +1.8 799,611 - +0.9 799,611 - +0.5 799,614 + -2.2 799,614 + -0.6 799,615 - -0.0 799,615 - -1.5 799,615 - +0.7 799,615 - -0.7 799,615 - -3.6 799,615 - +1.4 799,749 + +0.4 799,749 + +0.9 799,749 + -1.3 799,750 - +2.2 799,797 + +0.0 799,797 + -0.1 799,797 + +0.1 799,798 - +0.3 799,798 - -0.9 799,798 - +0.1 799,798 - +1.1 799,798 - +0.9 799,798 - +0.3 799,999 - Ac3H11_2523 0.18 +1.1 799,999 - Ac3H11_2523 0.18 +0.8 800,070 + Ac3H11_2523 0.22 -1.1 800,070 + Ac3H11_2523 0.22 +1.6 800,071 - Ac3H11_2523 0.22 -0.1 800,244 + Ac3H11_2523 0.30 +0.7 800,244 + Ac3H11_2523 0.30 -1.2 800,244 + Ac3H11_2523 0.30 +0.4 800,244 + Ac3H11_2523 0.30 -2.3 800,244 + Ac3H11_2523 0.30 -1.0 800,245 - Ac3H11_2523 0.30 +0.7 800,473 + Ac3H11_2523 0.41 +0.2 800,473 + Ac3H11_2523 0.41 -2.0 800,474 - Ac3H11_2523 0.41 +0.4 800,474 - Ac3H11_2523 0.41 +1.2 800,474 - Ac3H11_2523 0.41 -0.1 800,474 - Ac3H11_2523 0.41 -2.9 800,474 - Ac3H11_2523 0.41 -0.3 800,474 - Ac3H11_2523 0.41 +0.4 800,640 + Ac3H11_2523 0.49 +0.4 800,640 + Ac3H11_2523 0.49 +0.7 800,640 + Ac3H11_2523 0.49 +0.2 800,640 + Ac3H11_2523 0.49 +0.8 800,640 + Ac3H11_2523 0.49 -0.7 800,641 - Ac3H11_2523 0.49 +0.6 800,641 - Ac3H11_2523 0.49 -1.5 800,641 - Ac3H11_2523 0.49 +1.6 800,641 - Ac3H11_2523 0.49 -0.7 800,641 - Ac3H11_2523 0.49 -1.1 800,641 - Ac3H11_2523 0.49 -0.5 800,682 + Ac3H11_2523 0.51 -1.7 800,682 + Ac3H11_2523 0.51 -0.9 800,683 - Ac3H11_2523 0.51 +1.2 801,066 + Ac3H11_2523 0.69 +1.0 801,066 + Ac3H11_2523 0.69 -0.2 801,066 + Ac3H11_2523 0.69 +0.8 801,067 - Ac3H11_2523 0.69 +1.1 801,067 - Ac3H11_2523 0.69 +0.8 801,067 - Ac3H11_2523 0.69 -0.3 801,067 - Ac3H11_2523 0.69 -0.8 801,067 - Ac3H11_2523 0.69 -0.4 801,067 - Ac3H11_2523 0.69 +1.5 801,067 - Ac3H11_2523 0.69 +1.1 801,379 - Ac3H11_2523 0.84 +1.5 801,699 + -2.0 801,699 + +0.3 801,699 + +0.4 801,699 + +0.2 801,700 - -0.2 801,700 - +0.1 801,700 - +0.5 801,764 + +0.8 801,764 + +0.1 801,764 + +1.7 801,838 - -1.5 801,865 - +1.3 801,931 + +1.3 801,931 + -3.0 801,931 + -0.6 801,931 + +0.6 801,931 + -1.9 801,931 + -0.4 801,931 + -1.5 801,931 + -0.3 801,931 + +0.4 801,931 + -0.9 801,932 - +1.1 801,932 - -2.4 801,932 - +1.2 801,932 - +0.3 801,932 - -1.8 801,932 - +0.7 801,932 - -0.7 801,932 - +0.2 801,932 - -0.9 801,932 - -1.3 801,935 + +1.6 801,935 + -1.7 801,935 + -0.4 801,935 + -0.9 801,935 + -0.9 801,935 + -1.1 801,935 + +0.7 801,935 + +1.7 801,936 - +0.1 802,081 + +0.5 802,082 - Ac3H11_2524 0.26 -0.8 802,255 + Ac3H11_2524 0.51 -1.3 802,255 + Ac3H11_2524 0.51 +1.9 802,255 + Ac3H11_2524 0.51 -1.1 802,255 + Ac3H11_2524 0.51 -3.0 802,255 + Ac3H11_2524 0.51 +0.9 802,255 + Ac3H11_2524 0.51 -1.8 802,255 + Ac3H11_2524 0.51 -0.5 802,256 - Ac3H11_2524 0.51 -0.1 802,256 - Ac3H11_2524 0.51 -1.5 802,256 - Ac3H11_2524 0.51 -0.8 802,364 - Ac3H11_2524 0.66 -2.2 802,364 - Ac3H11_2524 0.66 -2.8 802,567 + +1.1 802,570 + -2.0 802,570 + -0.8 802,570 + -0.1 802,571 - -0.8 802,571 - -2.1 802,643 + -0.9 802,643 + -1.5 802,644 - -1.1 802,709 + Ac3H11_2525 0.14 -0.8 802,710 - Ac3H11_2525 0.14 -0.5 802,710 - Ac3H11_2525 0.14 -2.1 802,710 - Ac3H11_2525 0.14 +1.2
Or see this region's nucleotide sequence