Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1977 and Ac3H11_1978 are separated by 71 nucleotides Ac3H11_1978 and Ac3H11_2979 are separated by 312 nucleotides Ac3H11_2979 and Ac3H11_1979 are separated by 345 nucleotides
Ac3H11_1977 - Protein RtcB, at 189,431 to 190,702
_1977
Ac3H11_1978 - Putative stomatin/prohibitin-family membrane protease subunit aq_911, at 190,774 to 191,916
_1978
Ac3H11_2979 - tRNA-Undet-???, at 192,229 to 192,300
_2979
Ac3H11_1979 - Transcriptional regulatory protein RtcR, at 192,646 to 194,274
_1979
Position (kb)
190
191
192 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 189.810 kb on + strand, within Ac3H11_1977 at 189.810 kb on + strand, within Ac3H11_1977 at 189.810 kb on + strand, within Ac3H11_1977 at 189.810 kb on + strand, within Ac3H11_1977 at 189.811 kb on - strand, within Ac3H11_1977 at 189.811 kb on - strand, within Ac3H11_1977 at 189.951 kb on + strand, within Ac3H11_1977 at 189.951 kb on + strand, within Ac3H11_1977 at 189.951 kb on + strand, within Ac3H11_1977 at 189.951 kb on + strand, within Ac3H11_1977 at 189.951 kb on + strand, within Ac3H11_1977 at 189.951 kb on + strand, within Ac3H11_1977 at 189.951 kb on + strand, within Ac3H11_1977 at 189.952 kb on - strand, within Ac3H11_1977 at 189.964 kb on - strand, within Ac3H11_1977 at 189.985 kb on - strand, within Ac3H11_1977 at 190.124 kb on + strand, within Ac3H11_1977 at 190.176 kb on + strand, within Ac3H11_1977 at 190.176 kb on + strand, within Ac3H11_1977 at 190.176 kb on + strand, within Ac3H11_1977 at 190.176 kb on + strand, within Ac3H11_1977 at 190.176 kb on + strand, within Ac3H11_1977 at 190.176 kb on + strand, within Ac3H11_1977 at 190.177 kb on - strand, within Ac3H11_1977 at 190.177 kb on - strand, within Ac3H11_1977 at 190.177 kb on - strand, within Ac3H11_1977 at 190.177 kb on - strand, within Ac3H11_1977 at 190.632 kb on + strand at 190.632 kb on + strand at 190.632 kb on + strand at 190.632 kb on + strand at 190.641 kb on + strand at 190.641 kb on + strand at 190.642 kb on - strand at 191.112 kb on - strand, within Ac3H11_1978 at 191.249 kb on + strand, within Ac3H11_1978 at 191.250 kb on - strand, within Ac3H11_1978 at 191.250 kb on - strand, within Ac3H11_1978 at 191.255 kb on + strand, within Ac3H11_1978 at 191.255 kb on + strand, within Ac3H11_1978 at 191.255 kb on + strand, within Ac3H11_1978 at 191.255 kb on + strand, within Ac3H11_1978 at 191.256 kb on - strand, within Ac3H11_1978 at 191.387 kb on + strand, within Ac3H11_1978 at 191.387 kb on + strand, within Ac3H11_1978 at 191.388 kb on - strand, within Ac3H11_1978 at 191.388 kb on - strand, within Ac3H11_1978 at 191.603 kb on + strand, within Ac3H11_1978 at 191.735 kb on + strand, within Ac3H11_1978 at 191.736 kb on - strand, within Ac3H11_1978 at 191.786 kb on - strand, within Ac3H11_1978 at 191.786 kb on - strand, within Ac3H11_1978 at 191.819 kb on + strand at 191.819 kb on + strand at 191.819 kb on + strand at 191.820 kb on - strand at 191.820 kb on - strand at 191.820 kb on - strand at 192.208 kb on + strand at 192.208 kb on + strand at 192.209 kb on - strand at 192.279 kb on - strand, within Ac3H11_2979 at 192.291 kb on + strand, within Ac3H11_2979 at 192.292 kb on - strand, within Ac3H11_2979 at 192.292 kb on - strand, within Ac3H11_2979 at 192.292 kb on - strand, within Ac3H11_2979 at 192.292 kb on - strand, within Ac3H11_2979 at 192.449 kb on + strand at 192.522 kb on + strand at 192.522 kb on + strand at 192.522 kb on + strand at 192.522 kb on + strand at 192.522 kb on + strand at 192.523 kb on - strand at 192.523 kb on - strand at 192.523 kb on - strand at 192.523 kb on - strand at 192.523 kb on - strand at 192.614 kb on - strand at 192.633 kb on + strand at 192.787 kb on + strand at 192.787 kb on + strand at 192.788 kb on - strand at 192.788 kb on - strand at 192.910 kb on + strand, within Ac3H11_1979 at 192.910 kb on + strand, within Ac3H11_1979 at 192.910 kb on + strand, within Ac3H11_1979 at 192.910 kb on + strand, within Ac3H11_1979 at 192.910 kb on + strand, within Ac3H11_1979 at 192.910 kb on + strand, within Ac3H11_1979 at 192.910 kb on + strand, within Ac3H11_1979 at 192.911 kb on - strand, within Ac3H11_1979
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 189,810 + Ac3H11_1977 0.30 -2.6 189,810 + Ac3H11_1977 0.30 +0.9 189,810 + Ac3H11_1977 0.30 -0.8 189,810 + Ac3H11_1977 0.30 -0.4 189,811 - Ac3H11_1977 0.30 +1.6 189,811 - Ac3H11_1977 0.30 -3.9 189,951 + Ac3H11_1977 0.41 -0.4 189,951 + Ac3H11_1977 0.41 +0.1 189,951 + Ac3H11_1977 0.41 -0.7 189,951 + Ac3H11_1977 0.41 +0.7 189,951 + Ac3H11_1977 0.41 +1.2 189,951 + Ac3H11_1977 0.41 -1.8 189,951 + Ac3H11_1977 0.41 -0.1 189,952 - Ac3H11_1977 0.41 -2.4 189,964 - Ac3H11_1977 0.42 -4.2 189,985 - Ac3H11_1977 0.44 +0.2 190,124 + Ac3H11_1977 0.54 +1.0 190,176 + Ac3H11_1977 0.59 +0.9 190,176 + Ac3H11_1977 0.59 -2.3 190,176 + Ac3H11_1977 0.59 -0.0 190,176 + Ac3H11_1977 0.59 -1.3 190,176 + Ac3H11_1977 0.59 -1.1 190,176 + Ac3H11_1977 0.59 +1.4 190,177 - Ac3H11_1977 0.59 -0.0 190,177 - Ac3H11_1977 0.59 +0.5 190,177 - Ac3H11_1977 0.59 +0.4 190,177 - Ac3H11_1977 0.59 -1.0 190,632 + +0.1 190,632 + -1.0 190,632 + -0.3 190,632 + -0.4 190,641 + -0.1 190,641 + -2.0 190,642 - +1.7 191,112 - Ac3H11_1978 0.30 -0.3 191,249 + Ac3H11_1978 0.42 +1.0 191,250 - Ac3H11_1978 0.42 -0.5 191,250 - Ac3H11_1978 0.42 -0.8 191,255 + Ac3H11_1978 0.42 -0.5 191,255 + Ac3H11_1978 0.42 -0.7 191,255 + Ac3H11_1978 0.42 +0.0 191,255 + Ac3H11_1978 0.42 -0.8 191,256 - Ac3H11_1978 0.42 -0.2 191,387 + Ac3H11_1978 0.54 -0.1 191,387 + Ac3H11_1978 0.54 +0.6 191,388 - Ac3H11_1978 0.54 -1.3 191,388 - Ac3H11_1978 0.54 -0.8 191,603 + Ac3H11_1978 0.73 +1.8 191,735 + Ac3H11_1978 0.84 +1.0 191,736 - Ac3H11_1978 0.84 +1.0 191,786 - Ac3H11_1978 0.89 +0.2 191,786 - Ac3H11_1978 0.89 -1.0 191,819 + +0.1 191,819 + +0.5 191,819 + +0.4 191,820 - +0.7 191,820 - +1.2 191,820 - -0.4 192,208 + -0.8 192,208 + -2.6 192,209 - +0.3 192,279 - Ac3H11_2979 0.69 +0.5 192,291 + Ac3H11_2979 0.86 +0.5 192,292 - Ac3H11_2979 0.88 -1.5 192,292 - Ac3H11_2979 0.88 +0.4 192,292 - Ac3H11_2979 0.88 +0.7 192,292 - Ac3H11_2979 0.88 -1.0 192,449 + +1.6 192,522 + -0.2 192,522 + -3.6 192,522 + +0.8 192,522 + -0.8 192,522 + +0.8 192,523 - -3.3 192,523 - -0.5 192,523 - +0.9 192,523 - +0.4 192,523 - -1.0 192,614 - -0.3 192,633 + +1.2 192,787 + +1.0 192,787 + +0.4 192,788 - +0.4 192,788 - -3.2 192,910 + Ac3H11_1979 0.16 -1.8 192,910 + Ac3H11_1979 0.16 -0.1 192,910 + Ac3H11_1979 0.16 -1.2 192,910 + Ac3H11_1979 0.16 -4.0 192,910 + Ac3H11_1979 0.16 +0.8 192,910 + Ac3H11_1979 0.16 +1.6 192,910 + Ac3H11_1979 0.16 -2.6 192,911 - Ac3H11_1979 0.16 -1.8
Or see this region's nucleotide sequence