Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_178 and Ac3H11_179 are separated by 184 nucleotides Ac3H11_179 and Ac3H11_180 are separated by 196 nucleotides Ac3H11_180 and Ac3H11_181 are separated by 138 nucleotides
Ac3H11_178 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 66,255 to 67,439
_178
Ac3H11_179 - Tricarboxylate transport protein TctC, at 67,624 to 68,607
_179
Ac3H11_180 - Universal stress protein family, at 68,804 to 69,241
_180
Ac3H11_181 - FIG00538743: hypothetical protein, at 69,380 to 70,156
_181
Position (kb)
67
68
69 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 66.837 kb on + strand, within Ac3H11_178 at 66.838 kb on - strand, within Ac3H11_178 at 66.894 kb on + strand, within Ac3H11_178 at 66.894 kb on + strand, within Ac3H11_178 at 66.895 kb on - strand, within Ac3H11_178 at 66.895 kb on - strand, within Ac3H11_178 at 66.895 kb on - strand, within Ac3H11_178 at 66.895 kb on - strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.119 kb on + strand, within Ac3H11_178 at 67.120 kb on - strand, within Ac3H11_178 at 67.120 kb on - strand, within Ac3H11_178 at 67.120 kb on - strand, within Ac3H11_178 at 67.120 kb on - strand, within Ac3H11_178 at 67.278 kb on - strand, within Ac3H11_178 at 67.384 kb on + strand at 67.385 kb on - strand at 67.442 kb on + strand at 67.443 kb on - strand at 67.443 kb on - strand at 67.443 kb on - strand at 67.443 kb on - strand at 67.711 kb on + strand at 67.945 kb on + strand, within Ac3H11_179 at 67.945 kb on + strand, within Ac3H11_179 at 67.946 kb on - strand, within Ac3H11_179 at 67.946 kb on - strand, within Ac3H11_179 at 67.946 kb on - strand, within Ac3H11_179 at 67.946 kb on - strand, within Ac3H11_179 at 68.041 kb on + strand, within Ac3H11_179 at 68.041 kb on + strand, within Ac3H11_179 at 68.041 kb on + strand, within Ac3H11_179 at 68.042 kb on - strand, within Ac3H11_179 at 68.042 kb on - strand, within Ac3H11_179 at 68.042 kb on - strand, within Ac3H11_179 at 68.042 kb on - strand, within Ac3H11_179 at 68.098 kb on + strand, within Ac3H11_179 at 68.098 kb on + strand, within Ac3H11_179 at 68.098 kb on + strand, within Ac3H11_179 at 68.098 kb on + strand, within Ac3H11_179 at 68.098 kb on + strand, within Ac3H11_179 at 68.098 kb on + strand, within Ac3H11_179 at 68.098 kb on + strand, within Ac3H11_179 at 68.099 kb on - strand, within Ac3H11_179 at 68.099 kb on - strand, within Ac3H11_179 at 68.099 kb on - strand, within Ac3H11_179 at 68.099 kb on - strand, within Ac3H11_179 at 68.099 kb on - strand, within Ac3H11_179 at 68.099 kb on - strand, within Ac3H11_179 at 68.099 kb on - strand, within Ac3H11_179 at 68.099 kb on - strand, within Ac3H11_179 at 68.099 kb on - strand, within Ac3H11_179 at 68.099 kb on - strand, within Ac3H11_179 at 68.185 kb on + strand, within Ac3H11_179 at 68.185 kb on + strand, within Ac3H11_179 at 68.186 kb on - strand, within Ac3H11_179 at 68.390 kb on - strand, within Ac3H11_179 at 68.557 kb on + strand at 68.557 kb on + strand at 68.557 kb on + strand at 68.558 kb on - strand at 68.558 kb on - strand at 68.558 kb on - strand at 68.558 kb on - strand at 68.733 kb on - strand at 68.733 kb on - strand at 69.069 kb on + strand, within Ac3H11_180 at 69.069 kb on + strand, within Ac3H11_180 at 69.069 kb on + strand, within Ac3H11_180 at 69.070 kb on - strand, within Ac3H11_180 at 69.070 kb on - strand, within Ac3H11_180 at 69.070 kb on - strand, within Ac3H11_180 at 69.070 kb on - strand, within Ac3H11_180 at 69.070 kb on - strand, within Ac3H11_180 at 69.070 kb on - strand, within Ac3H11_180 at 69.084 kb on + strand, within Ac3H11_180 at 69.132 kb on + strand, within Ac3H11_180 at 69.132 kb on + strand, within Ac3H11_180 at 69.132 kb on + strand, within Ac3H11_180 at 69.132 kb on + strand, within Ac3H11_180 at 69.132 kb on + strand, within Ac3H11_180 at 69.132 kb on + strand, within Ac3H11_180 at 69.133 kb on - strand, within Ac3H11_180 at 69.133 kb on - strand, within Ac3H11_180 at 69.133 kb on - strand, within Ac3H11_180 at 69.133 kb on - strand, within Ac3H11_180 at 69.133 kb on - strand, within Ac3H11_180 at 69.247 kb on + strand at 69.247 kb on + strand at 69.247 kb on + strand at 69.247 kb on + strand at 69.247 kb on + strand at 69.248 kb on - strand at 69.248 kb on - strand at 69.248 kb on - strand at 69.248 kb on - strand at 69.248 kb on - strand at 69.254 kb on + strand at 69.255 kb on - strand at 69.255 kb on - strand at 69.255 kb on - strand at 69.255 kb on - strand at 69.277 kb on + strand at 69.277 kb on + strand at 69.278 kb on - strand at 69.278 kb on - strand at 69.278 kb on - strand at 69.333 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 66,837 + Ac3H11_178 0.49 +0.2 66,838 - Ac3H11_178 0.49 +0.2 66,894 + Ac3H11_178 0.54 -0.3 66,894 + Ac3H11_178 0.54 -3.0 66,895 - Ac3H11_178 0.54 -0.4 66,895 - Ac3H11_178 0.54 -0.2 66,895 - Ac3H11_178 0.54 +2.2 66,895 - Ac3H11_178 0.54 -1.2 67,119 + Ac3H11_178 0.73 +2.9 67,119 + Ac3H11_178 0.73 -1.0 67,119 + Ac3H11_178 0.73 +0.7 67,119 + Ac3H11_178 0.73 +0.5 67,119 + Ac3H11_178 0.73 -0.6 67,119 + Ac3H11_178 0.73 -0.7 67,119 + Ac3H11_178 0.73 +1.2 67,120 - Ac3H11_178 0.73 -0.8 67,120 - Ac3H11_178 0.73 -0.2 67,120 - Ac3H11_178 0.73 -0.9 67,120 - Ac3H11_178 0.73 +0.4 67,278 - Ac3H11_178 0.86 +0.3 67,384 + -0.8 67,385 - +0.3 67,442 + -1.8 67,443 - -2.2 67,443 - -3.1 67,443 - -0.4 67,443 - +1.3 67,711 + -2.3 67,945 + Ac3H11_179 0.33 +1.2 67,945 + Ac3H11_179 0.33 +0.5 67,946 - Ac3H11_179 0.33 -2.6 67,946 - Ac3H11_179 0.33 +1.7 67,946 - Ac3H11_179 0.33 +0.6 67,946 - Ac3H11_179 0.33 +0.5 68,041 + Ac3H11_179 0.42 +1.7 68,041 + Ac3H11_179 0.42 +0.7 68,041 + Ac3H11_179 0.42 -2.3 68,042 - Ac3H11_179 0.42 +0.4 68,042 - Ac3H11_179 0.42 -2.4 68,042 - Ac3H11_179 0.42 -1.0 68,042 - Ac3H11_179 0.42 +1.6 68,098 + Ac3H11_179 0.48 +0.6 68,098 + Ac3H11_179 0.48 +0.4 68,098 + Ac3H11_179 0.48 +1.4 68,098 + Ac3H11_179 0.48 +1.4 68,098 + Ac3H11_179 0.48 -0.7 68,098 + Ac3H11_179 0.48 +0.8 68,098 + Ac3H11_179 0.48 -1.4 68,099 - Ac3H11_179 0.48 -2.1 68,099 - Ac3H11_179 0.48 -0.8 68,099 - Ac3H11_179 0.48 +0.8 68,099 - Ac3H11_179 0.48 +0.7 68,099 - Ac3H11_179 0.48 -2.1 68,099 - Ac3H11_179 0.48 -2.1 68,099 - Ac3H11_179 0.48 +0.7 68,099 - Ac3H11_179 0.48 -1.1 68,099 - Ac3H11_179 0.48 -1.3 68,099 - Ac3H11_179 0.48 +0.2 68,185 + Ac3H11_179 0.57 +0.9 68,185 + Ac3H11_179 0.57 -2.2 68,186 - Ac3H11_179 0.57 -3.0 68,390 - Ac3H11_179 0.78 +1.0 68,557 + +0.3 68,557 + +0.8 68,557 + -0.5 68,558 - +2.0 68,558 - -0.2 68,558 - -0.8 68,558 - +2.7 68,733 - -1.4 68,733 - -3.8 69,069 + Ac3H11_180 0.61 +0.6 69,069 + Ac3H11_180 0.61 +1.0 69,069 + Ac3H11_180 0.61 +1.6 69,070 - Ac3H11_180 0.61 -1.1 69,070 - Ac3H11_180 0.61 -0.1 69,070 - Ac3H11_180 0.61 -0.3 69,070 - Ac3H11_180 0.61 +0.1 69,070 - Ac3H11_180 0.61 -0.8 69,070 - Ac3H11_180 0.61 -1.5 69,084 + Ac3H11_180 0.64 +0.5 69,132 + Ac3H11_180 0.75 +2.7 69,132 + Ac3H11_180 0.75 -0.1 69,132 + Ac3H11_180 0.75 +0.7 69,132 + Ac3H11_180 0.75 -1.1 69,132 + Ac3H11_180 0.75 +2.0 69,132 + Ac3H11_180 0.75 -0.6 69,133 - Ac3H11_180 0.75 +0.4 69,133 - Ac3H11_180 0.75 +1.3 69,133 - Ac3H11_180 0.75 -1.0 69,133 - Ac3H11_180 0.75 -1.7 69,133 - Ac3H11_180 0.75 +1.0 69,247 + -0.1 69,247 + -3.8 69,247 + +0.6 69,247 + +0.5 69,247 + +0.4 69,248 - -0.2 69,248 - +1.1 69,248 - +0.2 69,248 - +0.4 69,248 - -0.5 69,254 + -3.0 69,255 - -1.9 69,255 - -3.0 69,255 - -1.4 69,255 - +0.5 69,277 + +0.4 69,277 + +0.0 69,278 - -0.4 69,278 - +1.1 69,278 - -1.2 69,333 - +0.8
Or see this region's nucleotide sequence