Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1346 and Ac3H11_1347 are separated by 178 nucleotides Ac3H11_1347 and Ac3H11_1348 are separated by 48 nucleotides Ac3H11_1348 and Ac3H11_1349 are separated by 48 nucleotides
Ac3H11_1346 - Ser-tRNA(Ala) deacylase; Gly-tRNA(Ala) deacylase, at 39,078 to 39,830
_1346
Ac3H11_1347 - hypothetical protein, at 40,009 to 40,308
_1347
Ac3H11_1348 - Creatinine amidohydrolase (EC 3.5.2.10), at 40,357 to 41,220
_1348
Ac3H11_1349 - hypothetical protein, at 41,269 to 41,745
_1349
Position (kb) 40
41 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 39.727 kb on + strand, within Ac3H11_1346 at 39.800 kb on - strand at 39.881 kb on + strand at 39.882 kb on - strand at 39.882 kb on - strand at 39.882 kb on - strand at 39.939 kb on + strand at 39.940 kb on - strand at 39.945 kb on + strand at 39.945 kb on + strand at 39.946 kb on - strand at 40.072 kb on + strand, within Ac3H11_1347 at 40.072 kb on + strand, within Ac3H11_1347 at 40.072 kb on + strand, within Ac3H11_1347 at 40.073 kb on - strand, within Ac3H11_1347 at 40.073 kb on - strand, within Ac3H11_1347 at 40.073 kb on - strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.087 kb on + strand, within Ac3H11_1347 at 40.088 kb on - strand, within Ac3H11_1347 at 41.277 kb on - strand at 41.277 kb on - strand at 41.279 kb on + strand at 41.279 kb on + strand at 41.280 kb on - strand at 41.280 kb on - strand at 41.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 39,727 + Ac3H11_1346 0.86 -1.2 39,800 - -3.1 39,881 + +1.9 39,882 - -0.3 39,882 - +0.2 39,882 - -0.8 39,939 + -1.3 39,940 - -0.3 39,945 + -0.3 39,945 + -0.8 39,946 - +0.2 40,072 + Ac3H11_1347 0.21 -0.9 40,072 + Ac3H11_1347 0.21 +0.9 40,072 + Ac3H11_1347 0.21 -1.5 40,073 - Ac3H11_1347 0.21 +0.2 40,073 - Ac3H11_1347 0.21 -2.3 40,073 - Ac3H11_1347 0.21 -0.8 40,087 + Ac3H11_1347 0.26 +2.3 40,087 + Ac3H11_1347 0.26 -1.5 40,087 + Ac3H11_1347 0.26 +0.0 40,087 + Ac3H11_1347 0.26 -0.6 40,087 + Ac3H11_1347 0.26 +0.5 40,087 + Ac3H11_1347 0.26 -2.1 40,088 - Ac3H11_1347 0.26 -1.8 41,277 - -0.1 41,277 - +0.4 41,279 + -1.1 41,279 + -1.4 41,280 - +1.0 41,280 - -0.3 41,280 - +0.1
Or see this region's nucleotide sequence