Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1118 and Ac3H11_1119 are separated by 138 nucleotides Ac3H11_1119 and Ac3H11_1120 are separated by 45 nucleotides Ac3H11_1120 and Ac3H11_1121 are separated by 11 nucleotides
Ac3H11_1118 - Deoxycytidine triphosphate deaminase (EC 3.5.4.13), at 61,178 to 61,750
_1118
Ac3H11_1119 - hypothetical protein, at 61,889 to 62,170
_1119
Ac3H11_1120 - Diguanylate cyclase/phosphodiesterase domain 1 (GGDEF), at 62,216 to 63,916
_1120
Ac3H11_1121 - Ornithine cyclodeaminase (EC 4.3.1.12), at 63,928 to 64,941
_1121
Position (kb) 62
63
64 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 61.790 kb on + strand at 61.790 kb on + strand at 61.791 kb on - strand at 61.791 kb on - strand at 61.838 kb on + strand at 61.839 kb on - strand at 61.918 kb on + strand, within Ac3H11_1119 at 61.918 kb on + strand, within Ac3H11_1119 at 61.919 kb on - strand, within Ac3H11_1119 at 61.921 kb on + strand, within Ac3H11_1119 at 61.921 kb on + strand, within Ac3H11_1119 at 61.921 kb on + strand, within Ac3H11_1119 at 61.922 kb on - strand, within Ac3H11_1119 at 61.922 kb on - strand, within Ac3H11_1119 at 61.922 kb on - strand, within Ac3H11_1119 at 61.973 kb on + strand, within Ac3H11_1119 at 61.973 kb on + strand, within Ac3H11_1119 at 61.974 kb on - strand, within Ac3H11_1119 at 61.974 kb on - strand, within Ac3H11_1119 at 61.974 kb on - strand, within Ac3H11_1119 at 61.974 kb on - strand, within Ac3H11_1119 at 62.067 kb on - strand, within Ac3H11_1119 at 62.144 kb on + strand at 62.145 kb on - strand at 62.145 kb on - strand at 62.145 kb on - strand at 62.263 kb on - strand at 62.550 kb on + strand, within Ac3H11_1120 at 62.551 kb on - strand, within Ac3H11_1120 at 62.551 kb on - strand, within Ac3H11_1120 at 62.907 kb on + strand, within Ac3H11_1120 at 62.907 kb on + strand, within Ac3H11_1120 at 62.907 kb on + strand, within Ac3H11_1120 at 62.907 kb on + strand, within Ac3H11_1120 at 62.908 kb on - strand, within Ac3H11_1120 at 62.908 kb on - strand, within Ac3H11_1120 at 62.908 kb on - strand, within Ac3H11_1120 at 63.109 kb on - strand, within Ac3H11_1120 at 63.109 kb on - strand, within Ac3H11_1120 at 63.109 kb on - strand, within Ac3H11_1120 at 63.177 kb on + strand, within Ac3H11_1120 at 63.177 kb on + strand, within Ac3H11_1120 at 63.177 kb on + strand, within Ac3H11_1120 at 63.177 kb on + strand, within Ac3H11_1120 at 63.177 kb on + strand, within Ac3H11_1120 at 63.177 kb on + strand, within Ac3H11_1120 at 63.177 kb on + strand, within Ac3H11_1120 at 63.177 kb on + strand, within Ac3H11_1120 at 63.177 kb on + strand, within Ac3H11_1120 at 63.178 kb on - strand, within Ac3H11_1120 at 63.178 kb on - strand, within Ac3H11_1120 at 63.178 kb on - strand at 63.178 kb on - strand, within Ac3H11_1120 at 63.178 kb on - strand, within Ac3H11_1120 at 64.901 kb on + strand at 64.901 kb on + strand at 64.901 kb on + strand at 64.901 kb on + strand at 64.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 61,790 + -0.5 61,790 + -0.2 61,791 - -0.4 61,791 - -1.3 61,838 + +0.7 61,839 - +1.0 61,918 + Ac3H11_1119 0.10 +0.6 61,918 + Ac3H11_1119 0.10 -1.4 61,919 - Ac3H11_1119 0.11 -1.9 61,921 + Ac3H11_1119 0.11 -1.4 61,921 + Ac3H11_1119 0.11 -1.3 61,921 + Ac3H11_1119 0.11 +0.5 61,922 - Ac3H11_1119 0.12 -0.0 61,922 - Ac3H11_1119 0.12 +1.1 61,922 - Ac3H11_1119 0.12 -0.2 61,973 + Ac3H11_1119 0.30 +0.2 61,973 + Ac3H11_1119 0.30 -0.4 61,974 - Ac3H11_1119 0.30 -0.3 61,974 - Ac3H11_1119 0.30 -2.5 61,974 - Ac3H11_1119 0.30 +0.5 61,974 - Ac3H11_1119 0.30 -0.8 62,067 - Ac3H11_1119 0.63 -1.1 62,144 + -2.0 62,145 - -1.5 62,145 - -1.4 62,145 - +0.7 62,263 - -0.3 62,550 + Ac3H11_1120 0.20 +0.7 62,551 - Ac3H11_1120 0.20 +1.6 62,551 - Ac3H11_1120 0.20 -2.4 62,907 + Ac3H11_1120 0.41 -2.5 62,907 + Ac3H11_1120 0.41 +0.8 62,907 + Ac3H11_1120 0.41 -1.8 62,907 + Ac3H11_1120 0.41 +0.7 62,908 - Ac3H11_1120 0.41 +0.6 62,908 - Ac3H11_1120 0.41 -1.2 62,908 - Ac3H11_1120 0.41 -2.5 63,109 - Ac3H11_1120 0.52 -1.4 63,109 - Ac3H11_1120 0.52 -0.6 63,109 - Ac3H11_1120 0.52 -1.8 63,177 + Ac3H11_1120 0.56 -0.4 63,177 + Ac3H11_1120 0.56 -0.7 63,177 + Ac3H11_1120 0.56 +1.6 63,177 + Ac3H11_1120 0.56 +2.6 63,177 + Ac3H11_1120 0.56 +0.1 63,177 + Ac3H11_1120 0.56 -0.4 63,177 + Ac3H11_1120 0.56 +0.5 63,177 + Ac3H11_1120 0.56 -0.6 63,177 + Ac3H11_1120 0.56 -0.8 63,178 - Ac3H11_1120 0.57 +0.9 63,178 - Ac3H11_1120 0.57 +0.8 63,178 - +1.6 63,178 - Ac3H11_1120 0.57 -0.1 63,178 - Ac3H11_1120 0.57 -0.1 64,901 + -1.1 64,901 + -2.0 64,901 + -1.6 64,901 + -1.0 64,902 - -3.0
Or see this region's nucleotide sequence