Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3311 and Shew_3312 are separated by 30 nucleotides Shew_3312 and Shew_3313 are separated by 2 nucleotides Shew_3313 and Shew_3314 overlap by 4 nucleotides Shew_3314 and Shew_3315 overlap by 8 nucleotides
Shew_3311: Shew_3311 - RNA polymerase factor sigma-54 (RefSeq), at 3,951,593 to 3,953,080
_3311
Shew_3312: Shew_3312 - sigma 54 modulation protein/ribosomal protein S30EA (RefSeq), at 3,953,111 to 3,953,398
_3312
Shew_3313: Shew_3313 - PTS IIA-like nitrogen-regulatory protein PtsN (RefSeq), at 3,953,401 to 3,953,844
_3313
Shew_3314: Shew_3314 - hypothetical protein (RefSeq), at 3,953,841 to 3,954,689
_3314
Shew_3315: Shew_3315 - phosphotransferase system, phosphocarrier protein HPr (RefSeq), at 3,954,682 to 3,954,957
_3315
Position (kb) 3953
3954 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 3952.738 kb on + strand, within Shew_3311 at 3953.051 kb on - strand at 3953.217 kb on - strand, within Shew_3312 at 3953.569 kb on + strand, within Shew_3313 at 3953.569 kb on + strand, within Shew_3313 at 3954.150 kb on - strand, within Shew_3314 at 3954.661 kb on - strand at 3954.696 kb on - strand at 3954.761 kb on - strand, within Shew_3315
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 3,952,738 + Shew_3311 0.77 +2.1 3,953,051 - +1.9 3,953,217 - Shew_3312 0.37 -2.3 3,953,569 + Shew_3313 0.38 -2.8 3,953,569 + Shew_3313 0.38 -4.6 3,954,150 - Shew_3314 0.36 -3.2 3,954,661 - -0.1 3,954,696 - -1.4 3,954,761 - Shew_3315 0.29 -1.8
Or see this region's nucleotide sequence