Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3280 and rplI are separated by 77 nucleotides rplI and Shew_3282 are separated by 27 nucleotides Shew_3282 and Shew_3283 are separated by 12 nucleotides Shew_3283 and rpsF are separated by 95 nucleotides rpsF and Shew_3285 are separated by 222 nucleotides
Shew_3280: Shew_3280 - hypothetical protein (RefSeq), at 3,925,049 to 3,925,486
_3280
Shew_3281: rplI - 50S ribosomal protein L9 (RefSeq), at 3,925,564 to 3,926,016
rplI
Shew_3282: Shew_3282 - ribosomal protein S18 (RefSeq), at 3,926,044 to 3,926,271
_3282
Shew_3283: Shew_3283 - single-strand binding protein/primosomal replication protein N (RefSeq), at 3,926,284 to 3,926,502
_3283
Shew_3284: rpsF - 30S ribosomal protein S6 (RefSeq), at 3,926,598 to 3,927,005
rpsF
Shew_3285: Shew_3285 - hypothetical protein (RefSeq), at 3,927,228 to 3,927,971
_3285
Position (kb) 3926
3927 Strain fitness (log2 ratio) -1
0
1
2 at 3925.302 kb on - strand, within Shew_3280 at 3925.365 kb on + strand, within Shew_3280 at 3925.458 kb on - strand at 3927.270 kb on - strand at 3927.358 kb on - strand, within Shew_3285 at 3927.492 kb on + strand, within Shew_3285 at 3927.500 kb on - strand, within Shew_3285
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 3,925,302 - Shew_3280 0.58 +0.9 3,925,365 + Shew_3280 0.72 +2.1 3,925,458 - +0.1 3,927,270 - +1.1 3,927,358 - Shew_3285 0.17 +1.0 3,927,492 + Shew_3285 0.35 +0.3 3,927,500 - Shew_3285 0.37 +0.7
Or see this region's nucleotide sequence