Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2981 and Shew_2982 are separated by 126 nucleotides Shew_2982 and Shew_2983 are separated by 37 nucleotides Shew_2983 and Shew_2984 are separated by 205 nucleotides
Shew_2981: Shew_2981 - beta-lactamase domain-containing protein (RefSeq), at 3,535,567 to 3,537,531
_2981
Shew_2982: Shew_2982 - glucose-methanol-choline oxidoreductase (RefSeq), at 3,537,658 to 3,539,376
_2982
Shew_2983: Shew_2983 - Tat pathway signal sequence domain-containing protein (RefSeq), at 3,539,414 to 3,540,067
_2983
Shew_2984: Shew_2984 - ferredoxin-dependent glutamate synthase (RefSeq), at 3,540,273 to 3,541,823
_2984
Position (kb)
3537
3538
3539
3540 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3536.660 kb on + strand, within Shew_2981 at 3536.762 kb on + strand, within Shew_2981 at 3537.245 kb on - strand, within Shew_2981 at 3537.284 kb on + strand, within Shew_2981 at 3537.284 kb on + strand, within Shew_2981 at 3537.292 kb on - strand, within Shew_2981 at 3537.335 kb on - strand at 3537.422 kb on + strand at 3537.600 kb on + strand at 3537.608 kb on - strand at 3537.711 kb on + strand at 3537.802 kb on + strand at 3537.956 kb on + strand, within Shew_2982 at 3538.296 kb on - strand, within Shew_2982 at 3538.597 kb on - strand, within Shew_2982 at 3538.597 kb on - strand, within Shew_2982 at 3538.740 kb on + strand, within Shew_2982 at 3538.748 kb on - strand, within Shew_2982 at 3538.806 kb on - strand, within Shew_2982 at 3539.288 kb on - strand at 3539.614 kb on + strand, within Shew_2983 at 3539.744 kb on - strand, within Shew_2983 at 3539.939 kb on + strand, within Shew_2983
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 3,536,660 + Shew_2981 0.56 -0.0 3,536,762 + Shew_2981 0.61 -0.4 3,537,245 - Shew_2981 0.85 -0.1 3,537,284 + Shew_2981 0.87 -1.7 3,537,284 + Shew_2981 0.87 -0.9 3,537,292 - Shew_2981 0.88 -0.3 3,537,335 - -1.3 3,537,422 + -0.1 3,537,600 + +0.5 3,537,608 - +0.8 3,537,711 + +0.7 3,537,802 + -0.4 3,537,956 + Shew_2982 0.17 -0.4 3,538,296 - Shew_2982 0.37 +0.5 3,538,597 - Shew_2982 0.55 +0.2 3,538,597 - Shew_2982 0.55 -0.6 3,538,740 + Shew_2982 0.63 +1.0 3,538,748 - Shew_2982 0.63 -1.6 3,538,806 - Shew_2982 0.67 -0.1 3,539,288 - +1.4 3,539,614 + Shew_2983 0.31 -0.0 3,539,744 - Shew_2983 0.50 +0.7 3,539,939 + Shew_2983 0.80 -3.1
Or see this region's nucleotide sequence