Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2817 and Shew_2818 are separated by 177 nucleotides Shew_2818 and Shew_2819 overlap by 20 nucleotides Shew_2819 and prfC are separated by 256 nucleotides
Shew_2817: Shew_2817 - nucleoside transporter (RefSeq), at 3,338,227 to 3,339,486
_2817
Shew_2818: Shew_2818 - TatD-related deoxyribonuclease (RefSeq), at 3,339,664 to 3,340,494
_2818
Shew_2819: Shew_2819 - hypothetical protein (RefSeq), at 3,340,475 to 3,340,765
_2819
Shew_2820: prfC - peptide chain release factor 3 (RefSeq), at 3,341,022 to 3,342,602
prfC
Position (kb) 3339
3340
3341 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 3339.338 kb on + strand, within Shew_2817 at 3339.470 kb on - strand at 3339.985 kb on + strand, within Shew_2818 at 3340.149 kb on + strand, within Shew_2818 at 3340.308 kb on + strand, within Shew_2818 at 3340.334 kb on + strand, within Shew_2818 at 3340.334 kb on + strand, within Shew_2818 at 3340.503 kb on + strand at 3340.575 kb on - strand, within Shew_2819 at 3340.603 kb on + strand, within Shew_2819 at 3340.740 kb on - strand at 3340.778 kb on + strand at 3340.791 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 3,339,338 + Shew_2817 0.88 +2.8 3,339,470 - +2.0 3,339,985 + Shew_2818 0.39 -1.9 3,340,149 + Shew_2818 0.58 -0.7 3,340,308 + Shew_2818 0.77 -2.2 3,340,334 + Shew_2818 0.81 -1.4 3,340,334 + Shew_2818 0.81 +0.6 3,340,503 + +0.5 3,340,575 - Shew_2819 0.34 -0.1 3,340,603 + Shew_2819 0.44 +0.3 3,340,740 - +0.8 3,340,778 + +0.3 3,340,791 + +0.0
Or see this region's nucleotide sequence