Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2734 and Shew_2735 are separated by 257 nucleotides Shew_2735 and Shew_2736 are separated by 48 nucleotides Shew_2736 and Shew_2737 are separated by 202 nucleotides
Shew_2734: Shew_2734 - 4-carboxymuconolactone decarboxylase (RefSeq), at 3,231,868 to 3,232,251
_2734
Shew_2735: Shew_2735 - patatin (RefSeq), at 3,232,509 to 3,233,423
_2735
Shew_2736: Shew_2736 - MarR family transcriptional regulator (RefSeq), at 3,233,472 to 3,233,948
_2736
Shew_2737: Shew_2737 - AraC family transcriptional regulator (RefSeq), at 3,234,151 to 3,234,939
_2737
Position (kb)
3232
3233
3234 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3231.513 kb on + strand at 3231.677 kb on - strand at 3231.718 kb on + strand at 3231.799 kb on - strand at 3231.823 kb on - strand at 3231.829 kb on + strand at 3231.868 kb on + strand at 3232.233 kb on - strand at 3232.401 kb on + strand at 3232.401 kb on + strand at 3232.409 kb on - strand at 3232.437 kb on + strand at 3232.437 kb on + strand at 3232.471 kb on + strand at 3232.474 kb on - strand at 3232.474 kb on - strand at 3232.479 kb on - strand at 3232.638 kb on + strand, within Shew_2735 at 3232.768 kb on + strand, within Shew_2735 at 3233.117 kb on - strand, within Shew_2735 at 3233.230 kb on - strand, within Shew_2735 at 3233.739 kb on + strand, within Shew_2736 at 3233.844 kb on - strand, within Shew_2736 at 3234.227 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 3,231,513 + +1.4 3,231,677 - -0.8 3,231,718 + -0.4 3,231,799 - +1.4 3,231,823 - +0.7 3,231,829 + -0.1 3,231,868 + -1.4 3,232,233 - -0.0 3,232,401 + +0.2 3,232,401 + +0.9 3,232,409 - +0.7 3,232,437 + -0.1 3,232,437 + -3.8 3,232,471 + -0.3 3,232,474 - -0.1 3,232,474 - -1.4 3,232,479 - -0.1 3,232,638 + Shew_2735 0.14 +1.3 3,232,768 + Shew_2735 0.28 +0.7 3,233,117 - Shew_2735 0.66 +1.0 3,233,230 - Shew_2735 0.79 -0.1 3,233,739 + Shew_2736 0.56 +1.4 3,233,844 - Shew_2736 0.78 -0.1 3,234,227 + +1.6
Or see this region's nucleotide sequence