Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2590 and Shew_2591 are separated by 9 nucleotides Shew_2591 and Shew_2592 are separated by 403 nucleotides Shew_2592 and Shew_2593 are separated by 214 nucleotides
Shew_2590: Shew_2590 - hexapaptide repeat-containing transferase (RefSeq), at 3,056,013 to 3,056,642
_2590
Shew_2591: Shew_2591 - hypothetical protein (RefSeq), at 3,056,652 to 3,056,915
_2591
Shew_2592: Shew_2592 - peptidase M28 (RefSeq), at 3,057,319 to 3,058,905
_2592
Shew_2593: Shew_2593 - acyl-CoA synthetase (RefSeq), at 3,059,120 to 3,060,844
_2593
Position (kb)
3057
3058
3059 Strain fitness (log2 ratio)
-1
0
1 at 3056.946 kb on + strand at 3056.954 kb on - strand at 3056.988 kb on - strand at 3057.034 kb on - strand at 3057.221 kb on - strand at 3057.221 kb on - strand at 3057.760 kb on + strand, within Shew_2592 at 3057.819 kb on + strand, within Shew_2592 at 3058.692 kb on - strand, within Shew_2592 at 3058.856 kb on + strand at 3059.729 kb on - strand, within Shew_2593 at 3059.811 kb on - strand, within Shew_2593 at 3059.816 kb on + strand, within Shew_2593 at 3059.824 kb on - strand, within Shew_2593 at 3059.893 kb on + strand, within Shew_2593
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 3,056,946 + -0.7 3,056,954 - -0.1 3,056,988 - -1.0 3,057,034 - -1.5 3,057,221 - +0.6 3,057,221 - +0.5 3,057,760 + Shew_2592 0.28 +0.5 3,057,819 + Shew_2592 0.32 +1.0 3,058,692 - Shew_2592 0.87 +0.1 3,058,856 + +0.4 3,059,729 - Shew_2593 0.35 +1.2 3,059,811 - Shew_2593 0.40 -0.1 3,059,816 + Shew_2593 0.40 -0.0 3,059,824 - Shew_2593 0.41 +0.2 3,059,893 + Shew_2593 0.45 -0.4
Or see this region's nucleotide sequence