Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2457 and Shew_2458 are separated by 239 nucleotides Shew_2458 and Shew_2459 are separated by 243 nucleotides Shew_2459 and Shew_2460 are separated by 106 nucleotides
Shew_2457: Shew_2457 - nuclease SbcCD, D subunit (RefSeq), at 2,889,519 to 2,890,661
_2457
Shew_2458: Shew_2458 - glutathione S-transferase domain-containing protein (RefSeq), at 2,890,901 to 2,891,761
_2458
Shew_2459: Shew_2459 - hypothetical protein (RefSeq), at 2,892,005 to 2,892,352
_2459
Shew_2460: Shew_2460 - hypothetical protein (RefSeq), at 2,892,459 to 2,893,502
_2460
Position (kb)
2890
2891
2892 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2889.977 kb on + strand, within Shew_2457 at 2890.029 kb on + strand, within Shew_2457 at 2890.222 kb on - strand, within Shew_2457 at 2890.404 kb on + strand, within Shew_2457 at 2890.604 kb on + strand at 2890.612 kb on - strand at 2890.754 kb on - strand at 2891.040 kb on + strand, within Shew_2458 at 2891.696 kb on + strand at 2891.728 kb on - strand at 2891.751 kb on - strand at 2891.837 kb on + strand at 2892.242 kb on + strand, within Shew_2459 at 2892.465 kb on - strand at 2892.581 kb on + strand, within Shew_2460 at 2892.582 kb on - strand, within Shew_2460 at 2892.589 kb on - strand, within Shew_2460
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,889,977 + Shew_2457 0.40 +0.4 2,890,029 + Shew_2457 0.45 +1.2 2,890,222 - Shew_2457 0.62 +0.6 2,890,404 + Shew_2457 0.77 -4.4 2,890,604 + +0.4 2,890,612 - +0.0 2,890,754 - +2.1 2,891,040 + Shew_2458 0.16 -0.4 2,891,696 + -0.1 2,891,728 - +0.2 2,891,751 - +1.0 2,891,837 + +0.8 2,892,242 + Shew_2459 0.68 -0.7 2,892,465 - +0.2 2,892,581 + Shew_2460 0.12 +0.9 2,892,582 - Shew_2460 0.12 +1.2 2,892,589 - Shew_2460 0.12 -3.8
Or see this region's nucleotide sequence