Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2382 and Shew_2383 are separated by 62 nucleotides Shew_2383 and Shew_2384 are separated by 255 nucleotides Shew_2384 and Shew_2385 are separated by 979 nucleotides
Shew_2382: Shew_2382 - cysteine synthase A (RefSeq), at 2,788,241 to 2,789,209
_2382
Shew_2383: Shew_2383 - TetR family transcriptional regulator (RefSeq), at 2,789,272 to 2,789,928
_2383
Shew_2384: Shew_2384 - O-methyltransferase family protein (RefSeq), at 2,790,184 to 2,791,251
_2384
Shew_2385: Shew_2385 - TonB-dependent receptor, plug (RefSeq), at 2,792,231 to 2,794,918
_2385
Position (kb)
2790
2791
2792 Strain fitness (log2 ratio)
-2
-1
0
1 at 2789.307 kb on + strand at 2789.315 kb on - strand at 2789.367 kb on - strand, within Shew_2383 at 2789.622 kb on + strand, within Shew_2383 at 2789.670 kb on + strand, within Shew_2383 at 2789.850 kb on + strand, within Shew_2383 at 2790.113 kb on + strand at 2790.147 kb on - strand at 2790.335 kb on + strand, within Shew_2384 at 2790.671 kb on - strand, within Shew_2384 at 2790.671 kb on - strand, within Shew_2384 at 2790.830 kb on - strand, within Shew_2384 at 2791.210 kb on + strand at 2791.235 kb on + strand at 2791.235 kb on + strand at 2791.243 kb on - strand at 2791.323 kb on + strand at 2791.366 kb on + strand at 2791.394 kb on - strand at 2791.479 kb on + strand at 2791.603 kb on - strand at 2792.189 kb on - strand at 2792.189 kb on - strand at 2792.189 kb on - strand at 2792.189 kb on - strand at 2792.229 kb on - strand at 2792.244 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,789,307 + +0.7 2,789,315 - +0.7 2,789,367 - Shew_2383 0.14 +0.4 2,789,622 + Shew_2383 0.53 -0.6 2,789,670 + Shew_2383 0.61 +1.2 2,789,850 + Shew_2383 0.88 +0.5 2,790,113 + -0.5 2,790,147 - -0.7 2,790,335 + Shew_2384 0.14 +0.6 2,790,671 - Shew_2384 0.46 +0.1 2,790,671 - Shew_2384 0.46 +1.6 2,790,830 - Shew_2384 0.60 +0.9 2,791,210 + -0.3 2,791,235 + -1.4 2,791,235 + +0.3 2,791,243 - +0.2 2,791,323 + -0.2 2,791,366 + -0.4 2,791,394 - -1.3 2,791,479 + +0.0 2,791,603 - +0.9 2,792,189 - -1.4 2,792,189 - +0.5 2,792,189 - +0.4 2,792,189 - -2.7 2,792,229 - +0.8 2,792,244 - -1.5
Or see this region's nucleotide sequence