Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2257 and Shew_2258 are separated by 93 nucleotides Shew_2258 and Shew_2259 are separated by 159 nucleotides Shew_2259 and Shew_2260 are separated by 386 nucleotides Shew_2260 and Shew_2261 overlap by 1 nucleotides
Shew_2257: Shew_2257 - HAD family hydrolase (RefSeq), at 2,637,166 to 2,637,789
_2257
Shew_2258: Shew_2258 - NADH:ubiquinone oxidoreductase complex I intermediate-associated protein 30 (RefSeq), at 2,637,883 to 2,638,413
_2258
Shew_2259: Shew_2259 - metal dependent phosphohydrolase (RefSeq), at 2,638,573 to 2,639,775
_2259
Shew_2260: Shew_2260 - intracellular septation protein A (RefSeq), at 2,640,162 to 2,640,707
_2260
Shew_2261: Shew_2261 - YciI-like protein (RefSeq), at 2,640,707 to 2,641,006
_2261
Position (kb)
2638
2639
2640 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2637.749 kb on - strand at 2637.761 kb on - strand at 2637.761 kb on - strand at 2637.778 kb on - strand at 2637.845 kb on + strand at 2637.853 kb on - strand at 2638.223 kb on + strand, within Shew_2258 at 2638.787 kb on + strand, within Shew_2259 at 2638.883 kb on - strand, within Shew_2259 at 2639.353 kb on - strand, within Shew_2259 at 2639.580 kb on - strand, within Shew_2259 at 2639.658 kb on - strand at 2640.032 kb on - strand at 2640.671 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,637,749 - -0.1 2,637,761 - +0.8 2,637,761 - -0.5 2,637,778 - +0.8 2,637,845 + +1.4 2,637,853 - +0.9 2,638,223 + Shew_2258 0.64 +0.0 2,638,787 + Shew_2259 0.18 +0.8 2,638,883 - Shew_2259 0.26 +1.0 2,639,353 - Shew_2259 0.65 -2.9 2,639,580 - Shew_2259 0.84 +0.6 2,639,658 - +0.6 2,640,032 - +0.6 2,640,671 + -0.3
Or see this region's nucleotide sequence