Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2200 and hisH overlap by 4 nucleotides hisH and Shew_2202 overlap by 4 nucleotides Shew_2202 and Shew_2203 overlap by 19 nucleotides
Shew_2200: Shew_2200 - imidazole glycerol-phosphate dehydratase/histidinol phosphatase (RefSeq), at 2,560,961 to 2,562,031
_2200
Shew_2201: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,562,028 to 2,562,702
hisH
Shew_2202: Shew_2202 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,562,699 to 2,563,436
_2202
Shew_2203: Shew_2203 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,563,418 to 2,564,191
_2203
Position (kb)
2562
2563 Strain fitness (log2 ratio)
-1
0
1 at 2561.293 kb on + strand, within Shew_2200 at 2561.399 kb on + strand, within Shew_2200 at 2561.417 kb on + strand, within Shew_2200 at 2561.425 kb on - strand, within Shew_2200 at 2561.499 kb on + strand, within Shew_2200 at 2561.750 kb on + strand, within Shew_2200 at 2561.758 kb on - strand, within Shew_2200 at 2562.347 kb on + strand, within hisH at 2562.722 kb on + strand at 2562.800 kb on + strand, within Shew_2202 at 2563.591 kb on - strand, within Shew_2203
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,561,293 + Shew_2200 0.31 +0.2 2,561,399 + Shew_2200 0.41 +0.8 2,561,417 + Shew_2200 0.43 +0.2 2,561,425 - Shew_2200 0.43 +0.3 2,561,499 + Shew_2200 0.50 +0.0 2,561,750 + Shew_2200 0.74 -0.4 2,561,758 - Shew_2200 0.74 -1.4 2,562,347 + hisH Shew_2201 0.47 +0.2 2,562,722 + +0.9 2,562,800 + Shew_2202 0.14 -0.9 2,563,591 - Shew_2203 0.22 +0.3
Or see this region's nucleotide sequence