Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2147 and Shew_2148 are separated by 229 nucleotides Shew_2148 and Shew_2149 are separated by 86 nucleotides Shew_2149 and Shew_2150 are separated by 136 nucleotides Shew_2150 and Shew_2151 are separated by 116 nucleotides
Shew_2147: Shew_2147 - hypothetical protein (RefSeq), at 2,493,419 to 2,494,345
_2147
Shew_2148: Shew_2148 - hypothetical protein (RefSeq), at 2,494,575 to 2,494,982
_2148
Shew_2149: Shew_2149 - ABC transporter (RefSeq), at 2,495,069 to 2,495,929
_2149
Shew_2150: Shew_2150 - integral membrane protein TerC (RefSeq), at 2,496,066 to 2,496,293
_2150
Shew_2151: Shew_2151 - hypothetical protein (RefSeq), at 2,496,410 to 2,496,787
_2151
Position (kb)
2495
2496 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2494.195 kb on + strand, within Shew_2147 at 2494.311 kb on - strand at 2494.378 kb on + strand at 2494.386 kb on - strand at 2494.684 kb on - strand, within Shew_2148 at 2495.032 kb on + strand at 2495.186 kb on - strand, within Shew_2149 at 2495.668 kb on + strand, within Shew_2149 at 2495.776 kb on - strand, within Shew_2149 at 2495.881 kb on - strand at 2496.151 kb on + strand, within Shew_2150 at 2496.197 kb on - strand, within Shew_2150 at 2496.267 kb on - strand, within Shew_2150 at 2496.440 kb on + strand at 2496.440 kb on + strand at 2496.495 kb on - strand, within Shew_2151
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,494,195 + Shew_2147 0.84 +0.6 2,494,311 - -2.4 2,494,378 + -1.1 2,494,386 - +0.2 2,494,684 - Shew_2148 0.27 +1.4 2,495,032 + -2.3 2,495,186 - Shew_2149 0.14 -2.3 2,495,668 + Shew_2149 0.70 +0.1 2,495,776 - Shew_2149 0.82 +2.9 2,495,881 - -0.7 2,496,151 + Shew_2150 0.37 -0.2 2,496,197 - Shew_2150 0.57 +0.7 2,496,267 - Shew_2150 0.88 +1.1 2,496,440 + +0.6 2,496,440 + +0.6 2,496,495 - Shew_2151 0.22 -0.1
Or see this region's nucleotide sequence