Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and Shew_2092 are separated by 4 nucleotides Shew_2092 and Shew_2093 are separated by 1 nucleotides Shew_2093 and Shew_2094 overlap by 8 nucleotides Shew_2094 and Shew_2095 overlap by 14 nucleotides
Shew_2091: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,418,226 to 2,419,344
thiH
Shew_2092: Shew_2092 - thiazole biosynthesis family protein (RefSeq), at 2,419,349 to 2,420,122
_2092
Shew_2093: Shew_2093 - thiamine biosynthesis protein ThiS (RefSeq), at 2,420,124 to 2,420,333
_2093
Shew_2094: Shew_2094 - UBA/THIF-type NAD/FAD binding protein (RefSeq), at 2,420,326 to 2,421,174
_2094
Shew_2095: Shew_2095 - phosphomethylpyrimidine kinase (RefSeq), at 2,421,161 to 2,422,906
_2095
Position (kb)
2420
2421
2422 Strain fitness (log2 ratio)
-1
0
1 at 2419.930 kb on + strand, within Shew_2092 at 2420.359 kb on + strand at 2420.367 kb on - strand at 2420.726 kb on + strand, within Shew_2094 at 2420.810 kb on + strand, within Shew_2094 at 2420.918 kb on + strand, within Shew_2094 at 2421.046 kb on - strand, within Shew_2094 at 2421.179 kb on - strand at 2421.413 kb on - strand, within Shew_2095 at 2421.438 kb on + strand, within Shew_2095 at 2421.899 kb on + strand, within Shew_2095
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,419,930 + Shew_2092 0.75 +0.4 2,420,359 + +0.1 2,420,367 - -0.7 2,420,726 + Shew_2094 0.47 +0.0 2,420,810 + Shew_2094 0.57 -0.1 2,420,918 + Shew_2094 0.70 -0.6 2,421,046 - Shew_2094 0.85 +0.0 2,421,179 - -0.4 2,421,413 - Shew_2095 0.14 +0.3 2,421,438 + Shew_2095 0.16 +1.1 2,421,899 + Shew_2095 0.42 +1.1
Or see this region's nucleotide sequence