Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1878 and Shew_1879 are separated by 38 nucleotides Shew_1879 and Shew_1880 are separated by 50 nucleotides Shew_1880 and Shew_1881 are separated by 192 nucleotides Shew_1881 and Shew_1882 are separated by 1 nucleotides
Shew_1878: Shew_1878 - 2-nitropropane dioxygenase, NPD (RefSeq), at 2,163,071 to 2,164,156
_1878
Shew_1879: Shew_1879 - GCN5-related N-acetyltransferase (RefSeq), at 2,164,195 to 2,164,740
_1879
Shew_1880: Shew_1880 - hypothetical protein (RefSeq), at 2,164,791 to 2,165,231
_1880
Shew_1881: Shew_1881 - hypothetical protein (RefSeq), at 2,165,424 to 2,165,825
_1881
Shew_1882: Shew_1882 - metal dependent phosphohydrolase (RefSeq), at 2,165,827 to 2,167,113
_1882
Position (kb) 2164
2165
2166 Strain fitness (log2 ratio) -1
0
1 at 2164.063 kb on + strand at 2164.063 kb on + strand at 2164.071 kb on - strand at 2164.733 kb on + strand at 2164.944 kb on - strand, within Shew_1880 at 2165.394 kb on + strand at 2165.573 kb on + strand, within Shew_1881 at 2166.006 kb on - strand, within Shew_1882
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,164,063 + +1.0 2,164,063 + -0.6 2,164,071 - -1.4 2,164,733 + -0.2 2,164,944 - Shew_1880 0.35 +0.8 2,165,394 + +0.2 2,165,573 + Shew_1881 0.37 -0.4 2,166,006 - Shew_1882 0.14 -0.4
Or see this region's nucleotide sequence