Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1869 and Shew_1870 are separated by 64 nucleotides Shew_1870 and Shew_1871 overlap by 4 nucleotides Shew_1871 and Shew_1872 are separated by 95 nucleotides
Shew_1869: Shew_1869 - hypothetical protein (RefSeq), at 2,147,862 to 2,148,554
_1869
Shew_1870: Shew_1870 - LacI family transcription regulator (RefSeq), at 2,148,619 to 2,149,647
_1870
Shew_1871: Shew_1871 - DNA polymerase III, epsilon subunit (RefSeq), at 2,149,644 to 2,150,258
_1871
Shew_1872: Shew_1872 - elongation factor G (RefSeq), at 2,150,354 to 2,152,438
_1872
Position (kb)
2148
2149
2150 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2147.856 kb on - strand at 2147.858 kb on + strand at 2147.925 kb on + strand at 2147.982 kb on + strand, within Shew_1869 at 2148.129 kb on - strand, within Shew_1869 at 2148.422 kb on + strand, within Shew_1869 at 2148.741 kb on - strand, within Shew_1870 at 2148.811 kb on - strand, within Shew_1870 at 2148.935 kb on - strand, within Shew_1870 at 2148.950 kb on - strand, within Shew_1870 at 2150.213 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,147,856 - -1.2 2,147,858 + +1.2 2,147,925 + -2.8 2,147,982 + Shew_1869 0.17 +0.8 2,148,129 - Shew_1869 0.39 -0.1 2,148,422 + Shew_1869 0.81 -0.1 2,148,741 - Shew_1870 0.12 +1.1 2,148,811 - Shew_1870 0.19 +0.3 2,148,935 - Shew_1870 0.31 +0.4 2,148,950 - Shew_1870 0.32 +0.7 2,150,213 + -0.3
Or see this region's nucleotide sequence