Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1823 and Shew_1824 are separated by 224 nucleotides Shew_1824 and uvrC are separated by 31 nucleotides uvrC and Shew_1826 are separated by 47 nucleotides Shew_1826 and Shew_R0061 are separated by 140 nucleotides Shew_R0061 and Shew_R0062 are separated by 12 nucleotides Shew_R0062 and Shew_R0063 are separated by 39 nucleotides
Shew_1823: Shew_1823 - putative AcnD-accessory protein PrpF (RefSeq), at 2,097,828 to 2,098,997
_1823
Shew_1824: Shew_1824 - response regulator (RefSeq), at 2,099,222 to 2,099,911
_1824
Shew_1825: uvrC - excinuclease ABC subunit C (RefSeq), at 2,099,943 to 2,101,772
uvrC
Shew_1826: Shew_1826 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (RefSeq), at 2,101,820 to 2,102,377
_1826
Shew_R0061: Shew_R0061 - tRNA-Gly (RefSeq), at 2,102,518 to 2,102,593
_R0061
Shew_R0062: Shew_R0062 - tRNA-Cys (RefSeq), at 2,102,606 to 2,102,679
_R0062
Shew_R0063: Shew_R0063 - tRNA-Leu (RefSeq), at 2,102,719 to 2,102,805
_R0063
Position (kb)
2099
2100
2101
2102 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2099.961 kb on - strand at 2100.461 kb on + strand, within uvrC at 2100.544 kb on + strand, within uvrC at 2100.978 kb on + strand, within uvrC at 2101.073 kb on + strand, within uvrC at 2101.496 kb on - strand, within uvrC
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,099,961 - -3.6 2,100,461 + uvrC Shew_1825 0.28 -6.0 2,100,544 + uvrC Shew_1825 0.33 -5.5 2,100,978 + uvrC Shew_1825 0.57 -6.4 2,101,073 + uvrC Shew_1825 0.62 -3.6 2,101,496 - uvrC Shew_1825 0.85 -4.4
Or see this region's nucleotide sequence