Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1677 and Shew_1678 are separated by 72 nucleotides Shew_1678 and Shew_1679 are separated by 60 nucleotides Shew_1679 and Shew_1680 are separated by 128 nucleotides Shew_1680 and Shew_1681 are separated by 218 nucleotides
Shew_1677: Shew_1677 - leucyl aminopeptidase (RefSeq), at 1,928,104 to 1,929,465
_1677
Shew_1678: Shew_1678 - hypothetical protein (RefSeq), at 1,929,538 to 1,929,966
_1678
Shew_1679: Shew_1679 - TetR family transcriptional regulator (RefSeq), at 1,930,027 to 1,930,584
_1679
Shew_1680: Shew_1680 - HPP family protein+B94 (RefSeq), at 1,930,713 to 1,931,183
_1680
Shew_1681: Shew_1681 - diguanylate cyclase with PAS/PAC sensor (RefSeq), at 1,931,402 to 1,932,892
_1681
Position (kb)
1930
1931 Strain fitness (log2 ratio)
-2
-1
0
1 at 1929.031 kb on - strand, within Shew_1677 at 1929.379 kb on - strand at 1929.379 kb on - strand at 1929.420 kb on + strand at 1929.656 kb on - strand, within Shew_1678 at 1930.090 kb on - strand, within Shew_1679 at 1930.370 kb on + strand, within Shew_1679 at 1930.689 kb on + strand at 1931.364 kb on + strand at 1931.420 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,929,031 - Shew_1677 0.68 +0.5 1,929,379 - +0.9 1,929,379 - -0.3 1,929,420 + +0.9 1,929,656 - Shew_1678 0.28 +0.5 1,930,090 - Shew_1679 0.11 -1.5 1,930,370 + Shew_1679 0.61 -1.8 1,930,689 + +0.5 1,931,364 + +0.5 1,931,420 - -0.3
Or see this region's nucleotide sequence