Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1534 and Shew_1535 are separated by 33 nucleotides Shew_1535 and Shew_1536 are separated by 88 nucleotides Shew_1536 and Shew_1537 are separated by 321 nucleotides
Shew_1534: Shew_1534 - integral membrane sensor hybrid histidine kinase (RefSeq), at 1,760,224 to 1,763,646
_1534
Shew_1535: Shew_1535 - fumarylacetoacetate (FAA) hydrolase (RefSeq), at 1,763,680 to 1,764,291
_1535
Shew_1536: Shew_1536 - putative chloramphenicol acetyltransferase (RefSeq), at 1,764,380 to 1,765,006
_1536
Shew_1537: Shew_1537 - FAD dependent oxidoreductase (RefSeq), at 1,765,328 to 1,766,617
_1537
Position (kb) 1764
1765
1766 Strain fitness (log2 ratio) -1
0
1
2 at 1763.670 kb on + strand at 1763.718 kb on + strand at 1764.012 kb on + strand, within Shew_1535 at 1764.158 kb on + strand, within Shew_1535 at 1764.213 kb on + strand, within Shew_1535 at 1764.503 kb on - strand, within Shew_1536 at 1764.805 kb on + strand, within Shew_1536 at 1764.813 kb on - strand, within Shew_1536 at 1765.487 kb on - strand, within Shew_1537 at 1765.717 kb on - strand, within Shew_1537 at 1765.864 kb on - strand, within Shew_1537
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,763,670 + +0.1 1,763,718 + +0.8 1,764,012 + Shew_1535 0.54 +0.8 1,764,158 + Shew_1535 0.78 +0.1 1,764,213 + Shew_1535 0.87 -1.4 1,764,503 - Shew_1536 0.20 -0.1 1,764,805 + Shew_1536 0.68 +0.5 1,764,813 - Shew_1536 0.69 +0.6 1,765,487 - Shew_1537 0.12 +1.4 1,765,717 - Shew_1537 0.30 +0.1 1,765,864 - Shew_1537 0.42 +1.8
Or see this region's nucleotide sequence