Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1506 and Shew_1507 are separated by 2 nucleotides Shew_1507 and Shew_1508 are separated by 73 nucleotides Shew_1508 and asnB are separated by 75 nucleotides
Shew_1506: Shew_1506 - glyoxalase/bleomycin resistance protein/dioxygenase (RefSeq), at 1,728,569 to 1,728,919
_1506
Shew_1507: Shew_1507 - 5-carboxymethyl-2-hydroxymuconate isomerase (RefSeq), at 1,728,922 to 1,729,269
_1507
Shew_1508: Shew_1508 - extracellular solute-binding protein (RefSeq), at 1,729,343 to 1,730,116
_1508
Shew_1509: asnB - asparagine synthetase B (RefSeq), at 1,730,192 to 1,731,850
asnB
Position (kb)
1729
1730
1731 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1728.578 kb on + strand at 1728.581 kb on - strand at 1729.587 kb on + strand, within Shew_1508 at 1729.595 kb on - strand, within Shew_1508 at 1729.595 kb on - strand, within Shew_1508 at 1729.605 kb on - strand, within Shew_1508 at 1730.202 kb on - strand at 1730.403 kb on + strand, within asnB at 1730.667 kb on - strand, within asnB at 1730.935 kb on + strand, within asnB at 1731.037 kb on - strand, within asnB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,728,578 + +0.5 1,728,581 - -0.9 1,729,587 + Shew_1508 0.32 +1.1 1,729,595 - Shew_1508 0.33 -0.8 1,729,595 - Shew_1508 0.33 -1.4 1,729,605 - Shew_1508 0.34 -0.3 1,730,202 - -0.4 1,730,403 + asnB Shew_1509 0.13 +3.8 1,730,667 - asnB Shew_1509 0.29 +1.0 1,730,935 + asnB Shew_1509 0.45 -0.3 1,731,037 - asnB Shew_1509 0.51 +2.8
Or see this region's nucleotide sequence