Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1503 and Shew_1504 are separated by 131 nucleotides Shew_1504 and Shew_1505 are separated by 71 nucleotides Shew_1505 and Shew_1506 are separated by 232 nucleotides Shew_1506 and Shew_1507 are separated by 2 nucleotides
Shew_1503: Shew_1503 - oxidoreductase FAD-binding subunit (RefSeq), at 1,726,005 to 1,726,997
_1503
Shew_1504: Shew_1504 - GCN5-related N-acetyltransferase (RefSeq), at 1,727,129 to 1,727,560
_1504
Shew_1505: Shew_1505 - hypothetical protein (RefSeq), at 1,727,632 to 1,728,336
_1505
Shew_1506: Shew_1506 - glyoxalase/bleomycin resistance protein/dioxygenase (RefSeq), at 1,728,569 to 1,728,919
_1506
Shew_1507: Shew_1507 - 5-carboxymethyl-2-hydroxymuconate isomerase (RefSeq), at 1,728,922 to 1,729,269
_1507
Position (kb)
1727
1728
1729 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1727.004 kb on - strand at 1727.192 kb on + strand, within Shew_1504 at 1727.712 kb on - strand, within Shew_1505 at 1727.827 kb on - strand, within Shew_1505 at 1727.829 kb on + strand, within Shew_1505 at 1727.837 kb on - strand, within Shew_1505 at 1727.998 kb on - strand, within Shew_1505 at 1728.188 kb on - strand, within Shew_1505 at 1728.578 kb on + strand at 1728.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,727,004 - +0.4 1,727,192 + Shew_1504 0.15 -2.7 1,727,712 - Shew_1505 0.11 -0.4 1,727,827 - Shew_1505 0.28 -3.7 1,727,829 + Shew_1505 0.28 -2.9 1,727,837 - Shew_1505 0.29 -1.9 1,727,998 - Shew_1505 0.52 -2.0 1,728,188 - Shew_1505 0.79 -2.4 1,728,578 + +0.5 1,728,581 - -0.9
Or see this region's nucleotide sequence