Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1443 and Shew_1444 are separated by 83 nucleotides Shew_1444 and Shew_1445 overlap by 1 nucleotides Shew_1445 and Shew_1446 are separated by 87 nucleotides
Shew_1443: Shew_1443 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 1,658,689 to 1,660,512
_1443
Shew_1444: Shew_1444 - dicarboxylate TRAP transporter (succinate, fumarate, L-malate, and alpha-ketoglutarate), large permease component (from data) , at 1,660,596 to 1,661,993
_1444
Shew_1445: Shew_1445 - dicarboxylate TRAP transporter (succinate, fumarate, L-malate, and alpha-ketoglutarate), small permease component (from data) , at 1,661,993 to 1,662,631
_1445
Shew_1446: Shew_1446 - dicarboxylate TRAP transporter (succinate, fumarate, L-malate, and alpha-ketoglutarate), solute receptor component (from data) , at 1,662,719 to 1,663,729
_1446
Position (kb)
1660
1661
1662 Strain fitness (log2 ratio)
-2
-1
0
1 at 1659.748 kb on + strand, within Shew_1443 at 1659.929 kb on + strand, within Shew_1443 at 1659.969 kb on + strand, within Shew_1443 at 1660.215 kb on + strand, within Shew_1443 at 1660.243 kb on + strand, within Shew_1443 at 1660.307 kb on - strand, within Shew_1443 at 1660.439 kb on - strand at 1660.834 kb on + strand, within Shew_1444 at 1661.729 kb on + strand, within Shew_1444 at 1661.837 kb on - strand, within Shew_1444 at 1661.986 kb on - strand at 1662.196 kb on + strand, within Shew_1445 at 1662.820 kb on + strand, within Shew_1446 at 1662.944 kb on - strand, within Shew_1446
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,659,748 + Shew_1443 0.58 +0.3 1,659,929 + Shew_1443 0.68 -1.8 1,659,969 + Shew_1443 0.70 -0.2 1,660,215 + Shew_1443 0.84 -0.0 1,660,243 + Shew_1443 0.85 +0.3 1,660,307 - Shew_1443 0.89 -1.9 1,660,439 - -1.8 1,660,834 + Shew_1444 0.17 +1.3 1,661,729 + Shew_1444 0.81 -2.6 1,661,837 - Shew_1444 0.89 +0.7 1,661,986 - +1.3 1,662,196 + Shew_1445 0.32 -0.6 1,662,820 + Shew_1446 0.10 +0.2 1,662,944 - Shew_1446 0.22 -0.3
Or see this region's nucleotide sequence