Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1321 and Shew_1322 are separated by 10 nucleotides Shew_1322 and Shew_1323 overlap by 1 nucleotides Shew_1323 and Shew_1324 overlap by 1 nucleotides Shew_1324 and Shew_1325 overlap by 4 nucleotides Shew_1325 and Shew_1326 are separated by 9 nucleotides
Shew_1321: Shew_1321 - methyltransferase type 12 (RefSeq), at 1,534,408 to 1,535,598
_1321
Shew_1322: Shew_1322 - short-chain dehydrogenase/reductase SDR (RefSeq), at 1,535,609 to 1,536,337
_1322
Shew_1323: Shew_1323 - acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II-like protein (RefSeq), at 1,536,337 to 1,537,479
_1323
Shew_1324: Shew_1324 - hypothetical protein (RefSeq), at 1,537,479 to 1,537,724
_1324
Shew_1325: Shew_1325 - acetyltransferase-like protein (RefSeq), at 1,537,721 to 1,538,287
_1325
Shew_1326: Shew_1326 - short-chain dehydrogenase/reductase SDR (RefSeq), at 1,538,297 to 1,539,058
_1326
Position (kb)
1536
1537
1538 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1535.385 kb on - strand, within Shew_1321 at 1535.443 kb on + strand, within Shew_1321 at 1535.603 kb on - strand at 1535.710 kb on + strand, within Shew_1322 at 1535.872 kb on - strand, within Shew_1322 at 1535.872 kb on - strand, within Shew_1322 at 1535.884 kb on - strand, within Shew_1322 at 1535.994 kb on + strand, within Shew_1322 at 1535.994 kb on + strand, within Shew_1322 at 1535.997 kb on - strand, within Shew_1322 at 1536.002 kb on - strand, within Shew_1322 at 1536.217 kb on + strand, within Shew_1322 at 1536.248 kb on + strand, within Shew_1322 at 1536.444 kb on - strand at 1536.651 kb on - strand, within Shew_1323 at 1537.015 kb on - strand, within Shew_1323 at 1537.572 kb on - strand, within Shew_1324 at 1537.572 kb on - strand, within Shew_1324 at 1537.867 kb on + strand, within Shew_1325 at 1537.880 kb on - strand, within Shew_1325 at 1537.883 kb on + strand, within Shew_1325 at 1537.976 kb on + strand, within Shew_1325 at 1538.063 kb on + strand, within Shew_1325 at 1538.177 kb on - strand, within Shew_1325 at 1538.245 kb on + strand at 1538.409 kb on - strand, within Shew_1326
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,535,385 - Shew_1321 0.82 +0.4 1,535,443 + Shew_1321 0.87 +0.9 1,535,603 - -3.3 1,535,710 + Shew_1322 0.14 -2.7 1,535,872 - Shew_1322 0.36 -2.8 1,535,872 - Shew_1322 0.36 -2.1 1,535,884 - Shew_1322 0.38 -1.9 1,535,994 + Shew_1322 0.53 -1.0 1,535,994 + Shew_1322 0.53 -2.0 1,535,997 - Shew_1322 0.53 -3.0 1,536,002 - Shew_1322 0.54 +0.3 1,536,217 + Shew_1322 0.83 -4.0 1,536,248 + Shew_1322 0.88 -3.6 1,536,444 - -0.7 1,536,651 - Shew_1323 0.27 -4.4 1,537,015 - Shew_1323 0.59 -2.5 1,537,572 - Shew_1324 0.38 -3.9 1,537,572 - Shew_1324 0.38 -2.9 1,537,867 + Shew_1325 0.26 -2.6 1,537,880 - Shew_1325 0.28 +0.0 1,537,883 + Shew_1325 0.29 +1.5 1,537,976 + Shew_1325 0.45 -1.3 1,538,063 + Shew_1325 0.60 -3.0 1,538,177 - Shew_1325 0.80 +0.8 1,538,245 + +0.1 1,538,409 - Shew_1326 0.15 -0.1
Or see this region's nucleotide sequence