Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1252 and Shew_1253 are separated by 89 nucleotides Shew_1253 and Shew_1254 are separated by 72 nucleotides Shew_1254 and Shew_1255 are separated by 109 nucleotides
Shew_1252: Shew_1252 - transposase (RefSeq), at 1,450,808 to 1,451,473
_1252
Shew_1253: Shew_1253 - retinol acyltransferase domain-containing protein (RefSeq), at 1,451,563 to 1,452,288
_1253
Shew_1254: Shew_1254 - phage integrase family protein (RefSeq), at 1,452,361 to 1,452,921
_1254
Shew_1255: Shew_1255 - hypothetical protein (RefSeq), at 1,453,031 to 1,453,435
_1255
Position (kb)
1451
1452
1453 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1450.593 kb on - strand at 1450.594 kb on - strand at 1450.613 kb on - strand at 1450.620 kb on + strand at 1450.620 kb on + strand at 1450.638 kb on - strand at 1450.688 kb on + strand at 1450.823 kb on - strand at 1450.834 kb on - strand at 1450.881 kb on + strand, within Shew_1252 at 1450.918 kb on + strand, within Shew_1252 at 1450.918 kb on + strand, within Shew_1252 at 1450.926 kb on - strand, within Shew_1252 at 1450.926 kb on - strand, within Shew_1252 at 1450.967 kb on - strand, within Shew_1252 at 1451.077 kb on + strand, within Shew_1252 at 1451.133 kb on + strand, within Shew_1252 at 1451.178 kb on - strand, within Shew_1252 at 1451.280 kb on - strand, within Shew_1252 at 1451.280 kb on - strand, within Shew_1252 at 1451.416 kb on + strand at 1451.416 kb on + strand at 1451.476 kb on + strand at 1451.532 kb on + strand at 1451.540 kb on - strand at 1451.559 kb on - strand at 1451.683 kb on - strand, within Shew_1253 at 1451.806 kb on + strand, within Shew_1253 at 1451.811 kb on + strand, within Shew_1253 at 1451.814 kb on - strand, within Shew_1253 at 1451.814 kb on - strand, within Shew_1253 at 1451.814 kb on - strand, within Shew_1253 at 1451.819 kb on - strand, within Shew_1253 at 1451.922 kb on + strand, within Shew_1253 at 1451.931 kb on + strand, within Shew_1253 at 1452.127 kb on - strand, within Shew_1253 at 1452.228 kb on + strand at 1452.228 kb on + strand at 1452.228 kb on + strand at 1452.310 kb on + strand at 1452.320 kb on + strand at 1452.333 kb on + strand at 1452.346 kb on - strand at 1452.536 kb on + strand, within Shew_1254 at 1452.794 kb on + strand, within Shew_1254 at 1452.804 kb on + strand, within Shew_1254 at 1452.804 kb on + strand, within Shew_1254
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,450,593 - +0.2 1,450,594 - +0.5 1,450,613 - -0.3 1,450,620 + -1.9 1,450,620 + -2.3 1,450,638 - -0.2 1,450,688 + +0.5 1,450,823 - +0.1 1,450,834 - -0.6 1,450,881 + Shew_1252 0.11 +0.6 1,450,918 + Shew_1252 0.17 +0.2 1,450,918 + Shew_1252 0.17 +0.3 1,450,926 - Shew_1252 0.18 -1.3 1,450,926 - Shew_1252 0.18 +0.1 1,450,967 - Shew_1252 0.24 +1.0 1,451,077 + Shew_1252 0.40 -3.0 1,451,133 + Shew_1252 0.49 -2.3 1,451,178 - Shew_1252 0.56 -0.5 1,451,280 - Shew_1252 0.71 +0.8 1,451,280 - Shew_1252 0.71 -0.9 1,451,416 + +0.3 1,451,416 + +1.8 1,451,476 + +0.6 1,451,532 + -0.6 1,451,540 - +0.8 1,451,559 - +1.1 1,451,683 - Shew_1253 0.17 +1.5 1,451,806 + Shew_1253 0.33 +0.1 1,451,811 + Shew_1253 0.34 -0.0 1,451,814 - Shew_1253 0.35 +0.5 1,451,814 - Shew_1253 0.35 +0.3 1,451,814 - Shew_1253 0.35 +0.1 1,451,819 - Shew_1253 0.35 +1.5 1,451,922 + Shew_1253 0.49 +0.9 1,451,931 + Shew_1253 0.51 -3.6 1,452,127 - Shew_1253 0.78 +1.6 1,452,228 + +0.1 1,452,228 + +0.5 1,452,228 + +0.7 1,452,310 + +0.6 1,452,320 + -0.3 1,452,333 + +0.3 1,452,346 - -0.1 1,452,536 + Shew_1254 0.31 +0.1 1,452,794 + Shew_1254 0.77 -0.1 1,452,804 + Shew_1254 0.79 +0.1 1,452,804 + Shew_1254 0.79 -1.3
Or see this region's nucleotide sequence