Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1240 and Shew_1241 are separated by 411 nucleotides Shew_1241 and Shew_1242 are separated by 152 nucleotides Shew_1242 and Shew_1243 are separated by 82 nucleotides
Shew_1240: Shew_1240 - hypothetical protein (RefSeq), at 1,440,028 to 1,440,336
_1240
Shew_1241: Shew_1241 - sigma 54 modulation protein/ribosomal protein S30EA (RefSeq), at 1,440,748 to 1,441,104
_1241
Shew_1242: Shew_1242 - hypothetical protein (RefSeq), at 1,441,257 to 1,441,730
_1242
Shew_1243: Shew_1243 - peptidyl-Lys metalloendopeptidase (RefSeq), at 1,441,813 to 1,442,898
_1243
Position (kb)
1441
1442 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1440.580 kb on + strand at 1440.580 kb on + strand at 1440.588 kb on - strand at 1441.239 kb on + strand at 1441.286 kb on + strand at 1441.319 kb on + strand, within Shew_1242 at 1441.327 kb on - strand, within Shew_1242 at 1441.331 kb on + strand, within Shew_1242 at 1441.339 kb on + strand, within Shew_1242 at 1441.432 kb on + strand, within Shew_1242 at 1441.617 kb on - strand, within Shew_1242 at 1441.713 kb on + strand at 1441.917 kb on - strand at 1442.020 kb on - strand, within Shew_1243 at 1442.289 kb on + strand, within Shew_1243 at 1442.289 kb on + strand, within Shew_1243 at 1442.360 kb on + strand, within Shew_1243 at 1442.425 kb on + strand, within Shew_1243 at 1442.510 kb on - strand, within Shew_1243 at 1442.570 kb on + strand, within Shew_1243 at 1442.653 kb on + strand, within Shew_1243
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,440,580 + +0.2 1,440,580 + -0.3 1,440,588 - +0.6 1,441,239 + +0.3 1,441,286 + +0.8 1,441,319 + Shew_1242 0.13 +1.0 1,441,327 - Shew_1242 0.15 +1.6 1,441,331 + Shew_1242 0.16 +0.3 1,441,339 + Shew_1242 0.17 +1.7 1,441,432 + Shew_1242 0.37 +0.1 1,441,617 - Shew_1242 0.76 +0.5 1,441,713 + -0.1 1,441,917 - +0.7 1,442,020 - Shew_1243 0.19 -2.0 1,442,289 + Shew_1243 0.44 +1.9 1,442,289 + Shew_1243 0.44 +2.1 1,442,360 + Shew_1243 0.50 +3.5 1,442,425 + Shew_1243 0.56 -0.5 1,442,510 - Shew_1243 0.64 +0.6 1,442,570 + Shew_1243 0.70 +0.0 1,442,653 + Shew_1243 0.77 +0.3
Or see this region's nucleotide sequence