Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1207 and Shew_1208 are separated by 3 nucleotides Shew_1208 and Shew_1209 are separated by 9 nucleotides Shew_1209 and Shew_1210 overlap by 8 nucleotides Shew_1210 and Shew_1211 overlap by 19 nucleotides
Shew_1207: Shew_1207 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (RefSeq), at 1,402,081 to 1,402,809
_1207
Shew_1208: Shew_1208 - 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (RefSeq), at 1,402,813 to 1,403,298
_1208
Shew_1209: Shew_1209 - pseudouridylate synthase (RefSeq), at 1,403,308 to 1,404,375
_1209
Shew_1210: Shew_1210 - stationary-phase survival protein SurE (RefSeq), at 1,404,368 to 1,405,117
_1210
Shew_1211: Shew_1211 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,405,099 to 1,405,749
_1211
Position (kb) 1403
1404
1405 Strain fitness (log2 ratio) -1
0
1
2
3 at 1403.277 kb on + strand at 1403.544 kb on + strand, within Shew_1209 at 1403.561 kb on + strand, within Shew_1209 at 1403.659 kb on - strand, within Shew_1209 at 1403.700 kb on - strand, within Shew_1209 at 1403.738 kb on - strand, within Shew_1209 at 1404.427 kb on + strand at 1404.481 kb on + strand, within Shew_1210 at 1404.569 kb on - strand, within Shew_1210 at 1405.267 kb on + strand, within Shew_1211 at 1405.302 kb on + strand, within Shew_1211 at 1405.310 kb on - strand, within Shew_1211
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,403,277 + +1.1 1,403,544 + Shew_1209 0.22 +0.3 1,403,561 + Shew_1209 0.24 +0.7 1,403,659 - Shew_1209 0.33 +1.7 1,403,700 - Shew_1209 0.37 +0.6 1,403,738 - Shew_1209 0.40 +3.1 1,404,427 + +1.8 1,404,481 + Shew_1210 0.15 +1.2 1,404,569 - Shew_1210 0.27 -0.3 1,405,267 + Shew_1211 0.26 +0.4 1,405,302 + Shew_1211 0.31 +0.1 1,405,310 - Shew_1211 0.32 +0.5
Or see this region's nucleotide sequence