Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gltB and Shew_1033 are separated by 430 nucleotides Shew_1033 and Shew_1034 are separated by 173 nucleotides Shew_1034 and Shew_1035 are separated by 205 nucleotides
Shew_1032: gltB - glutamate synthase subunit alpha (RefSeq), at 1,190,911 to 1,195,359
gltB
Shew_1033: Shew_1033 - putative radical SAM protein (RefSeq), at 1,195,790 to 1,196,782
_1033
Shew_1034: Shew_1034 - Hpt protein (RefSeq), at 1,196,956 to 1,197,321
_1034
Shew_1035: Shew_1035 - LysR family transcriptional regulator (RefSeq), at 1,197,527 to 1,198,444
_1035
Position (kb)
1195
1196
1197 Strain fitness (log2 ratio)
-2
-1
0
1 at 1194.851 kb on + strand, within gltB at 1194.859 kb on - strand, within gltB at 1194.939 kb on - strand at 1194.984 kb on + strand at 1195.706 kb on + strand at 1195.860 kb on + strand at 1195.977 kb on + strand, within Shew_1033 at 1196.029 kb on + strand, within Shew_1033 at 1196.071 kb on + strand, within Shew_1033 at 1196.126 kb on - strand, within Shew_1033 at 1196.178 kb on + strand, within Shew_1033 at 1196.267 kb on - strand, within Shew_1033 at 1196.307 kb on + strand, within Shew_1033 at 1196.657 kb on - strand, within Shew_1033 at 1197.416 kb on + strand at 1197.452 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,194,851 + gltB Shew_1032 0.89 +0.0 1,194,859 - gltB Shew_1032 0.89 -0.3 1,194,939 - -2.4 1,194,984 + -2.0 1,195,706 + -2.3 1,195,860 + -0.3 1,195,977 + Shew_1033 0.19 +0.6 1,196,029 + Shew_1033 0.24 -2.0 1,196,071 + Shew_1033 0.28 -0.9 1,196,126 - Shew_1033 0.34 -0.7 1,196,178 + Shew_1033 0.39 -1.8 1,196,267 - Shew_1033 0.48 -0.0 1,196,307 + Shew_1033 0.52 -1.4 1,196,657 - Shew_1033 0.87 +0.7 1,197,416 + +0.5 1,197,452 + -1.5
Or see this region's nucleotide sequence