Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0843 and nrfA are separated by 247 nucleotides nrfA and Shew_0845 are separated by 74 nucleotides Shew_0845 and Shew_0846 are separated by 252 nucleotides
Shew_0843: Shew_0843 - nitrate/nitrite sensor protein NarQ (RefSeq), at 965,163 to 966,866
_0843
Shew_0844: nrfA - cytochrome c nitrite reductase (RefSeq), at 967,114 to 968,514
nrfA
Shew_0845: Shew_0845 - isochorismatase hydrolase (RefSeq), at 968,589 to 969,131
_0845
Shew_0846: Shew_0846 - nitrogen regulatory protein P-II (RefSeq), at 969,384 to 969,722
_0846
Position (kb) 967
968
969 Strain fitness (log2 ratio) -2
-1
0
1
2 at 966.200 kb on - strand, within Shew_0843 at 966.292 kb on + strand, within Shew_0843 at 966.571 kb on - strand, within Shew_0843 at 967.147 kb on - strand at 967.202 kb on + strand at 967.210 kb on - strand at 967.256 kb on - strand, within nrfA at 967.270 kb on + strand, within nrfA at 967.341 kb on - strand, within nrfA at 967.395 kb on + strand, within nrfA at 967.480 kb on - strand, within nrfA at 967.498 kb on + strand, within nrfA at 967.541 kb on - strand, within nrfA at 967.579 kb on - strand, within nrfA at 967.595 kb on + strand, within nrfA at 967.686 kb on + strand, within nrfA at 967.721 kb on + strand, within nrfA at 967.791 kb on + strand, within nrfA at 967.911 kb on + strand, within nrfA at 968.054 kb on + strand, within nrfA at 968.141 kb on - strand, within nrfA at 968.361 kb on + strand, within nrfA at 968.361 kb on + strand, within nrfA at 968.524 kb on + strand at 968.634 kb on - strand at 968.762 kb on + strand, within Shew_0845 at 968.981 kb on + strand, within Shew_0845 at 969.098 kb on - strand at 969.379 kb on - strand at 969.447 kb on + strand, within Shew_0846 at 969.505 kb on + strand, within Shew_0846
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 966,200 - Shew_0843 0.61 +1.1 966,292 + Shew_0843 0.66 +0.4 966,571 - Shew_0843 0.83 +1.3 967,147 - -1.0 967,202 + +1.2 967,210 - -1.4 967,256 - nrfA Shew_0844 0.10 -0.5 967,270 + nrfA Shew_0844 0.11 -0.7 967,341 - nrfA Shew_0844 0.16 -1.3 967,395 + nrfA Shew_0844 0.20 +0.1 967,480 - nrfA Shew_0844 0.26 +0.8 967,498 + nrfA Shew_0844 0.27 -0.6 967,541 - nrfA Shew_0844 0.30 +0.1 967,579 - nrfA Shew_0844 0.33 +0.6 967,595 + nrfA Shew_0844 0.34 -0.2 967,686 + nrfA Shew_0844 0.41 +0.3 967,721 + nrfA Shew_0844 0.43 +0.3 967,791 + nrfA Shew_0844 0.48 -0.0 967,911 + nrfA Shew_0844 0.57 -0.1 968,054 + nrfA Shew_0844 0.67 +0.4 968,141 - nrfA Shew_0844 0.73 -2.3 968,361 + nrfA Shew_0844 0.89 +0.5 968,361 + nrfA Shew_0844 0.89 -0.5 968,524 + +0.1 968,634 - -1.4 968,762 + Shew_0845 0.32 +0.7 968,981 + Shew_0845 0.72 -0.2 969,098 - +0.2 969,379 - -0.4 969,447 + Shew_0846 0.19 +2.0 969,505 + Shew_0846 0.36 +1.2
Or see this region's nucleotide sequence