Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0825 and Shew_0826 are separated by 330 nucleotides Shew_0826 and Shew_0827 are separated by 110 nucleotides Shew_0827 and Shew_0828 are separated by 4 nucleotides
Shew_0825: Shew_0825 - ribose-5-phosphate isomerase A (RefSeq), at 948,036 to 948,695
_0825
Shew_0826: Shew_0826 - hypothetical protein (RefSeq), at 949,026 to 949,949
_0826
Shew_0827: Shew_0827 - peptidyl-prolyl cis-trans isomerase, cyclophilin type (RefSeq), at 950,060 to 950,707
_0827
Shew_0828: Shew_0828 - LysR family transcriptional regulator (RefSeq), at 950,712 to 951,605
_0828
Position (kb) 949
950 Strain fitness (log2 ratio) -2
-1
0
1 at 948.699 kb on - strand at 948.752 kb on + strand at 948.911 kb on + strand at 949.226 kb on + strand, within Shew_0826 at 949.761 kb on + strand, within Shew_0826 at 949.796 kb on + strand, within Shew_0826 at 949.845 kb on + strand, within Shew_0826 at 949.853 kb on - strand, within Shew_0826 at 950.145 kb on - strand, within Shew_0827 at 950.676 kb on + strand at 950.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 948,699 - -2.5 948,752 + +0.5 948,911 + -0.8 949,226 + Shew_0826 0.22 -0.3 949,761 + Shew_0826 0.80 +0.9 949,796 + Shew_0826 0.83 -0.3 949,845 + Shew_0826 0.89 +0.6 949,853 - Shew_0826 0.90 +1.3 950,145 - Shew_0827 0.13 -0.1 950,676 + -0.2 950,696 - +0.1
Or see this region's nucleotide sequence