Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0771 and Shew_0772 are separated by 119 nucleotides Shew_0772 and Shew_0773 are separated by 138 nucleotides
Shew_0771: Shew_0771 - thiol:disulfide interchange protein DsbC (RefSeq), at 880,241 to 880,966
_0771
Shew_0772: Shew_0772 - single-stranded-DNA-specific exonuclease RecJ (RefSeq), at 881,086 to 882,810
_0772
Shew_0773: Shew_0773 - fructose-1,6-bisphosphatase (RefSeq), at 882,949 to 883,938
_0773
Position (kb) 881
882
883 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 880.377 kb on + strand, within Shew_0771 at 880.525 kb on - strand, within Shew_0771 at 880.550 kb on - strand, within Shew_0771 at 880.826 kb on - strand, within Shew_0771 at 880.860 kb on + strand, within Shew_0771 at 880.868 kb on - strand, within Shew_0771 at 880.868 kb on - strand, within Shew_0771 at 881.031 kb on + strand at 881.708 kb on + strand, within Shew_0772 at 881.900 kb on - strand, within Shew_0772 at 881.947 kb on - strand, within Shew_0772 at 882.102 kb on - strand, within Shew_0772 at 882.688 kb on + strand at 882.742 kb on - strand at 882.788 kb on - strand at 882.808 kb on - strand at 882.844 kb on - strand at 882.947 kb on + strand at 882.954 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 880,377 + Shew_0771 0.19 -1.2 880,525 - Shew_0771 0.39 -1.9 880,550 - Shew_0771 0.43 -0.4 880,826 - Shew_0771 0.81 -0.5 880,860 + Shew_0771 0.85 -0.6 880,868 - Shew_0771 0.86 -0.4 880,868 - Shew_0771 0.86 -1.6 881,031 + -0.4 881,708 + Shew_0772 0.36 -4.9 881,900 - Shew_0772 0.47 -2.7 881,947 - Shew_0772 0.50 -3.0 882,102 - Shew_0772 0.59 -1.9 882,688 + -3.5 882,742 - -6.3 882,788 - -1.2 882,808 - +0.1 882,844 - +0.0 882,947 + +1.1 882,954 + -1.1
Or see this region's nucleotide sequence