Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0770 and Shew_0771 are separated by 220 nucleotides Shew_0771 and Shew_0772 are separated by 119 nucleotides
Shew_0770: Shew_0770 - tyrosine recombinase XerD (RefSeq), at 879,109 to 880,020
_0770
Shew_0771: Shew_0771 - thiol:disulfide interchange protein DsbC (RefSeq), at 880,241 to 880,966
_0771
Shew_0772: Shew_0772 - single-stranded-DNA-specific exonuclease RecJ (RefSeq), at 881,086 to 882,810
_0772
Position (kb)
880
881 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 879.257 kb on + strand, within Shew_0770 at 879.354 kb on - strand, within Shew_0770 at 879.539 kb on + strand, within Shew_0770 at 879.549 kb on + strand, within Shew_0770 at 879.701 kb on + strand, within Shew_0770 at 879.803 kb on + strand, within Shew_0770 at 879.918 kb on + strand, within Shew_0770 at 879.993 kb on + strand at 880.008 kb on + strand at 880.377 kb on + strand, within Shew_0771 at 880.525 kb on - strand, within Shew_0771 at 880.550 kb on - strand, within Shew_0771 at 880.826 kb on - strand, within Shew_0771 at 880.860 kb on + strand, within Shew_0771 at 880.868 kb on - strand, within Shew_0771 at 880.868 kb on - strand, within Shew_0771 at 881.031 kb on + strand at 881.708 kb on + strand, within Shew_0772 at 881.900 kb on - strand, within Shew_0772 at 881.947 kb on - strand, within Shew_0772
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 879,257 + Shew_0770 0.16 -3.3 879,354 - Shew_0770 0.27 -2.7 879,539 + Shew_0770 0.47 -3.6 879,549 + Shew_0770 0.48 -3.2 879,701 + Shew_0770 0.65 -3.9 879,803 + Shew_0770 0.76 -3.6 879,918 + Shew_0770 0.89 -4.1 879,993 + -3.2 880,008 + -0.4 880,377 + Shew_0771 0.19 -1.2 880,525 - Shew_0771 0.39 -1.9 880,550 - Shew_0771 0.43 -0.4 880,826 - Shew_0771 0.81 -0.5 880,860 + Shew_0771 0.85 -0.6 880,868 - Shew_0771 0.86 -0.4 880,868 - Shew_0771 0.86 -1.6 881,031 + -0.4 881,708 + Shew_0772 0.36 -4.9 881,900 - Shew_0772 0.47 -2.7 881,947 - Shew_0772 0.50 -3.0
Or see this region's nucleotide sequence