Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0733 and Shew_0734 are separated by 237 nucleotides Shew_0734 and Shew_0735 are separated by 20 nucleotides Shew_0735 and Shew_0736 are separated by 104 nucleotides
Shew_0733: Shew_0733 - hypothetical protein (RefSeq), at 834,650 to 835,405
_0733
Shew_0734: Shew_0734 - Fe-S metabolism associated SufE (RefSeq), at 835,643 to 836,095
_0734
Shew_0735: Shew_0735 - SufS subfamily cysteine desulfurase (RefSeq), at 836,116 to 837,327
_0735
Shew_0736: Shew_0736 - aldo/keto reductase (RefSeq), at 837,432 to 838,472
_0736
Position (kb) 836
837
838 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 835.380 kb on + strand at 835.542 kb on + strand at 835.550 kb on - strand at 836.082 kb on - strand at 836.608 kb on - strand, within Shew_0735 at 836.762 kb on + strand, within Shew_0735 at 837.045 kb on - strand, within Shew_0735 at 837.097 kb on - strand, within Shew_0735 at 837.591 kb on + strand, within Shew_0736 at 837.967 kb on + strand, within Shew_0736 at 838.136 kb on - strand, within Shew_0736
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 835,380 + -0.9 835,542 + +0.1 835,550 - -0.6 836,082 - -3.8 836,608 - Shew_0735 0.41 -0.7 836,762 + Shew_0735 0.53 +1.1 837,045 - Shew_0735 0.77 +0.3 837,097 - Shew_0735 0.81 -2.1 837,591 + Shew_0736 0.15 +0.2 837,967 + Shew_0736 0.51 -0.5 838,136 - Shew_0736 0.68 -0.2
Or see this region's nucleotide sequence